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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srtScenarioUnspecifiedDomain" xlink:to="loc_KBLBFirstSixMonthsMember_30" xlink:type="arc" order="31"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_us-gaapDebtInstrumentTerm_80" xlink:type="arc" order="2"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_us-gaapRepaymentsOfDebt_80" xlink:type="arc" order="3"/> |
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| <link:definitionLink xlink:type="extended" xlink:role="http://kraiglabs.com/role/StockholdersDeficitDetailsNarrative" xlink:title="00000046 - Disclosure - Stockholders' Deficit (Details Narrative)"> |
| | <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems" xlink:label="loc_us-gaapStatementLineItems"/> |
| | <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable" xlink:label="loc_us-gaapStatementTable"/> |
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| | <link:loc xlink:type="locator" xlink:href="kblb-20200331.xsd#KBLB_PurchaseAgreementMember" xlink:label="loc_KBLBPurchaseAgreementMember_0"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_KBLBWarrantSharesFullyVested_650" xlink:type="arc" order="10"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_KBLBExtendedExpirationDateDescription_650" xlink:type="arc" order="12"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_us-gaapEquityMethodInvestmentOwnershipPercentage_650" xlink:type="arc" order="14"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_KBLBLicenseFee_650" xlink:type="arc" order="16"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_KBLBLicenseMaintenanceFee_650" xlink:type="arc" order="17"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_KBLBAnnualResearchFee_650" xlink:type="arc" order="18"/> |
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| | <link:loc xlink:type="locator" xlink:href="kblb-20200331.xsd#KBLB_RoyaltyPercentage" xlink:label="loc_KBLBRoyaltyPercentage_650"/> |
| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_KBLBRoyaltyPercentage_650" xlink:type="arc" order="20"/> |
| | <link:loc xlink:type="locator" xlink:href="kblb-20200331.xsd#KBLB_TerminationAgreementDescription" xlink:label="loc_KBLBTerminationAgreementDescription_650"/> |
| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_KBLBTerminationAgreementDescription_650" xlink:type="arc" order="21"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_us-gaapRepaymentsOfDebt_650" xlink:type="arc" order="22"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_us-gaapStockIssuedDuringPeriodValueNewIssues_650" xlink:type="arc" order="23"/> |
| | <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProfessionalFees" xlink:label="loc_us-gaapProfessionalFees_650"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_us-gaapDebtInstrumentMaturityDate_650" xlink:type="arc" order="27"/> |
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| | <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaapStatementLineItems" xlink:to="loc_us-gaapFairValueAdjustmentOfWarrants_650" xlink:type="arc" order="30"/> |
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| <link:definitionLink xlink:type="extended" xlink:role="http://kraiglabs.com/role/RelatedPartyTransactionsDetailsNarrative" xlink:title="00000054 - Disclosure - Related Party Transactions (Details Narrative)"> |
| | <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems" xlink:label="loc_us-gaapStatementLineItems"/> |
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