SEC Info℠  
    Home      Search      My Interests      Help      Sign In      Please Sign In

Aei Net Lease Income & Growth Fund XX Ltd. Partnership – ‘10-Q’ for 6/30/22 – ‘EX-101.DEF’

On:  Wednesday, 8/10/22, at 6:45pm ET   ·   As of:  8/11/22   ·   For:  6/30/22   ·   Accession #:  894245-22-98   ·   File #:  0-23778

Previous ‘10-Q’:  ‘10-Q’ on 5/13/22 for 3/31/22   ·   Next & Latest:  ‘10-Q’ on 11/14/22 for 9/30/22

Find Words in Filings emoji
 		  in    Show  and   ↓Hints

  As Of               Filer                 Filing    For·On·As Docs:Size

 8/11/22  Aei Net Lease Income & Gro… XX LP 10-Q        6/30/22   31:1.5M

Quarterly Report   —   Form 10-Q

Filing Table of Contents

Document/Exhibit                   Description                      Pages   Size 

 1: 10-Q        Inline XBRL Document                                HTML    316K 
 2: EX-31.1     Certification -- §302 - SOA'02                      HTML     14K 
 3: EX-31.2     Certification -- §302 - SOA'02                      HTML     14K 
 4: EX-32       Section 1350 Certifications                         HTML     13K 
10: R1          Document And Entity Information                     HTML     65K 
11: R2          Balance Sheet                                       HTML     78K 
12: R3          Balance Sheet (Parentheticals)                      HTML     16K 
13: R4          Statement of Income                                 HTML     60K 
14: R5          Statement of Cash Flows                             HTML     61K 
15: R6          Statement of Changes in Partners' Capital           HTML     46K 
16: R7          Basis of Accounting                                 HTML     13K 
17: R8          Organization                                        HTML     15K 
18: R9          Recently Issued Accounting Pronouncements           HTML     14K 
19: R10         Real Estate Investments                             HTML     17K 
20: R11         Payable to AEI Fund Management, Inc.                HTML     13K 
21: R12         Partners' Capital                                   HTML     14K 
22: R13         Fair Value Measurements                             HTML     15K 
23: R14         Accounting Policies, by Policy (Policies)           HTML     19K 
24: R15         Real Estate Investments (Details)                   HTML     71K 
25: R16         Partners' Capital (Details)                         HTML     36K 
26: R17         Fair Value Measurements (Details)                   HTML     20K 
29: XML         IDEA XML File -- Filing Summary                      XML     43K 
27: XML         XBRL Instance -- aei20-20220630_htm                  XML    217K 
28: EXCEL       IDEA Workbook of Financial Reports                  XLSX     40K 
 5: EX-101.CAL  XBRL Calculations -- aei20-20220630_cal              XML     58K 
 6: EX-101.DEF  XBRL Definitions -- aei20-20220630_def               XML    219K 
 7: EX-101.LAB  XBRL Labels -- aei20-20220630_lab                    XML    454K 
 8: EX-101.PRE  XBRL Presentations -- aei20-20220630_pre             XML    218K 
 9: EX-101.SCH  XBRL Schema -- aei20-20220630                        XSD     70K 
30: JSON        XBRL Instance as JSON Data -- MetaLinks              114±   160K 
31: ZIP         XBRL Zipped Folder -- 0000894245-22-000098-xbrl      Zip     76K

‘EX-101.DEF’   —   XBRL Definitions — aei20-20220630_def

This Exhibit is an XBRL XML File.

                                                                                                                                                                                
<?xml version="1.0" encoding="windows-1252"?>
<!-- Generated by CompSci Transform (tm) - http://www.compsciresources.com -->
<!-- Created: Wed Aug 10 22:44:41 UTC 2022 -->
<linkbase xmlns="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:xbrldt="http://xbrl.org/2005/xbrldt">
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_ConsolidatedBalanceSheet" roleURI="http://aeifunds.com/role/ConsolidatedBalanceSheet"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_ConsolidatedBalanceSheet_Parentheticals" roleURI="http://aeifunds.com/role/ConsolidatedBalanceSheet_Parentheticals"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_ConsolidatedIncomeStatement" roleURI="http://aeifunds.com/role/ConsolidatedIncomeStatement"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_ConsolidatedCashFlow" roleURI="http://aeifunds.com/role/ConsolidatedCashFlow"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_ShareholdersEquityType2or3" roleURI="http://aeifunds.com/role/ShareholdersEquityType2or3"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_BasisofAccounting" roleURI="http://aeifunds.com/role/BasisofAccounting"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_Organization" roleURI="http://aeifunds.com/role/Organization"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_RecentlyIssuedAccountingPronouncements" roleURI="http://aeifunds.com/role/RecentlyIssuedAccountingPronouncements"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_RealEstateInvestments" roleURI="http://aeifunds.com/role/RealEstateInvestments"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_PayabletoAEIFundManagementInc" roleURI="http://aeifunds.com/role/PayabletoAEIFundManagementInc"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_PartnersCapital" roleURI="http://aeifunds.com/role/PartnersCapital"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_FairValueMeasurements" roleURI="http://aeifunds.com/role/FairValueMeasurements"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_AccountingPoliciesByPolicy" roleURI="http://aeifunds.com/role/AccountingPoliciesByPolicy"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_RealEstateInvestmentsDetails" roleURI="http://aeifunds.com/role/RealEstateInvestmentsDetails"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_PartnersCapitalDetails" roleURI="http://aeifunds.com/role/PartnersCapitalDetails"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#aei20_r_FairValueMeasurementsDetails" roleURI="http://aeifunds.com/role/FairValueMeasurementsDetails"/>
<roleRef xlink:type="simple" xlink:href="aei20-20220630.xsd#DocumentAndEntityInformation" roleURI="http://aeifunds.com/role/DocumentAndEntityInformation"/>
<arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default"/>
<arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain"/>
<arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension"/>
<arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member"/>
<arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" arcroleURI="http://xbrl.org/int/dim/arcrole/all"/>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/ConsolidatedBalanceSheet">
<loc xlink:type="locator" xlink:label="us-gaap_StatementOfFinancialPositionAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementOfFinancialPositionAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_AssetsCurrentAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrentAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfFinancialPositionAbstract" xlink:to="us-gaap_AssetsCurrentAbstract" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_CashAndCashEquivalentsAtCarryingValue" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ReceivablesNetCurrent" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReceivablesNetCurrent"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_ReceivablesNetCurrent" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_AssetsCurrent" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ReceivablesNetCurrent" xlink:to="us-gaap_AssetsCurrent" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateInvestmentsAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateInvestmentsAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfFinancialPositionAbstract" xlink:to="us-gaap_RealEstateInvestmentsAbstract" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_Land" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Land"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RealEstateInvestmentsAbstract" xlink:to="us-gaap_Land" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_BuildingsAndImprovementsGross" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_BuildingsAndImprovementsGross"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RealEstateInvestmentsAbstract" xlink:to="us-gaap_BuildingsAndImprovementsGross" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_FiniteLivedIntangibleAssetsGross" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RealEstateInvestmentsAbstract" xlink:to="us-gaap_FiniteLivedIntangibleAssetsGross" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateInvestmentPropertyAtCost" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateInvestmentPropertyAtCost"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsGross" xlink:to="us-gaap_RealEstateInvestmentPropertyAtCost" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateInvestmentPropertyAccumulatedDepreciation" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateInvestmentPropertyAccumulatedDepreciation"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsGross" xlink:to="us-gaap_RealEstateInvestmentPropertyAccumulatedDepreciation" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateInvestmentPropertyNet" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateInvestmentPropertyNet"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RealEstateInvestmentPropertyAccumulatedDepreciation" xlink:to="us-gaap_RealEstateInvestmentPropertyNet" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_InventoryRealEstateHeldForSale" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryRealEstateHeldForSale"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsGross" xlink:to="us-gaap_InventoryRealEstateHeldForSale" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateInvestments" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateInvestments"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InventoryRealEstateHeldForSale" xlink:to="us-gaap_RealEstateInvestments" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_Assets" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Assets"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InventoryRealEstateHeldForSale" xlink:to="us-gaap_Assets" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesCurrentAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrentAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfFinancialPositionAbstract" xlink:to="us-gaap_LiabilitiesCurrentAbstract" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_AccountsPayableRelatedPartiesCurrent" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsPayableRelatedPartiesCurrent"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_AccountsPayableRelatedPartiesCurrent" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_DividendsPayableCurrent" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DividendsPayableCurrent"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_DividendsPayableCurrent" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesCurrent" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DividendsPayableCurrent" xlink:to="us-gaap_LiabilitiesCurrent" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnersCapitalAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnersCapitalAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfFinancialPositionAbstract" xlink:to="us-gaap_PartnersCapitalAbstract" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_GeneralPartnersCapitalAccount" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GeneralPartnersCapitalAccount"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnersCapitalAbstract" xlink:to="us-gaap_GeneralPartnersCapitalAccount" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LimitedPartnersCapitalAccount" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LimitedPartnersCapitalAccount"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnersCapitalAbstract" xlink:to="us-gaap_LimitedPartnersCapitalAccount" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnersCapital" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnersCapital"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LimitedPartnersCapitalAccount" xlink:to="us-gaap_PartnersCapital" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesAndStockholdersEquity" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnersCapital" xlink:to="us-gaap_LiabilitiesAndStockholdersEquity" order="0" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/ConsolidatedBalanceSheet_Parentheticals">
<loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
<loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountAxis"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain_0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain_0" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LimitedPartnerMember" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LimitedPartnerMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" xlink:to="us-gaap_LimitedPartnerMember" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LimitedPartnersCapitalAccountUnitsAuthorized" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LimitedPartnersCapitalAccountUnitsAuthorized"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_LimitedPartnersCapitalAccountUnitsAuthorized" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LimitedPartnersCapitalAccountUnitsIssued" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LimitedPartnersCapitalAccountUnitsIssued"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_LimitedPartnersCapitalAccountUnitsIssued" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LimitedPartnersCapitalAccountUnitsOutstanding" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LimitedPartnersCapitalAccountUnitsOutstanding"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_LimitedPartnersCapitalAccountUnitsOutstanding" order="3" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/ConsolidatedIncomeStatement">
<loc xlink:type="locator" xlink:label="us-gaap_IncomeStatementAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeStatementAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_OperatingLeaseLeaseIncome" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLeaseIncome"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeStatementAbstract" xlink:to="us-gaap_OperatingLeaseLeaseIncome" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_ExpensesAbstract" xlink:href="aei20-20220630.xsd#aei20_ExpensesAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeStatementAbstract" xlink:to="aei20_ExpensesAbstract" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_CostsAndExpensesRelatedParty" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostsAndExpensesRelatedParty"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_ExpensesAbstract" xlink:to="us-gaap_CostsAndExpensesRelatedParty" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_OperatingCostsAndExpenses" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingCostsAndExpenses"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_ExpensesAbstract" xlink:to="us-gaap_OperatingCostsAndExpenses" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_DepreciationAndAmortization" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepreciationAndAmortization"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_ExpensesAbstract" xlink:to="us-gaap_DepreciationAndAmortization" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ImpairmentOfRealEstate" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ImpairmentOfRealEstate"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_ExpensesAbstract" xlink:to="us-gaap_ImpairmentOfRealEstate" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_OperatingExpenses" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingExpenses"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ImpairmentOfRealEstate" xlink:to="us-gaap_OperatingExpenses" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_OperatingIncomeLoss" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeStatementAbstract" xlink:to="us-gaap_OperatingIncomeLoss" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_OtherIncomeAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherIncomeAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeStatementAbstract" xlink:to="us-gaap_OtherIncomeAbstract" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_GainLossOnDispositionOfAssets1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GainLossOnDispositionOfAssets1"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OtherIncomeAbstract" xlink:to="us-gaap_GainLossOnDispositionOfAssets1" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_InvestmentIncomeInterest" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentIncomeInterest"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OtherIncomeAbstract" xlink:to="us-gaap_InvestmentIncomeInterest" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_OtherIncome" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherIncome"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InvestmentIncomeInterest" xlink:to="us-gaap_OtherIncome" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ProfitLoss" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProfitLoss"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeStatementAbstract" xlink:to="us-gaap_ProfitLoss" order="4" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_NetIncomeLossAllocatedAbstract" xlink:href="aei20-20220630.xsd#aei20_NetIncomeLossAllocatedAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeStatementAbstract" xlink:to="aei20_NetIncomeLossAllocatedAbstract" order="5" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_NetIncomeLossAllocatedToGeneralPartners" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLossAllocatedToGeneralPartners"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_NetIncomeLossAllocatedAbstract" xlink:to="us-gaap_NetIncomeLossAllocatedToGeneralPartners" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_NetIncomeLossAllocatedToLimitedPartners" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLossAllocatedToLimitedPartners"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_NetIncomeLossAllocatedAbstract" xlink:to="us-gaap_NetIncomeLossAllocatedToLimitedPartners" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_IncomeLossFromContinuingOperationsPerOutstandingLimitedPartnershipUnitBasicNetOfTax" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsPerOutstandingLimitedPartnershipUnitBasicNetOfTax"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeStatementAbstract" xlink:to="us-gaap_IncomeLossFromContinuingOperationsPerOutstandingLimitedPartnershipUnitBasicNetOfTax" order="6" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_WeightedAverageGeneralPartnershipUnitsOutstanding" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageGeneralPartnershipUnitsOutstanding"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeStatementAbstract" xlink:to="us-gaap_WeightedAverageGeneralPartnershipUnitsOutstanding" order="7" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/ConsolidatedCashFlow">
<loc xlink:type="locator" xlink:label="us-gaap_StatementOfCashFlowsAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementOfCashFlowsAbstract"/>
<loc xlink:type="locator" xlink:label="aei20_CashFlowsFromOperatingActivitiesAbstract" xlink:href="aei20-20220630.xsd#aei20_CashFlowsFromOperatingActivitiesAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfCashFlowsAbstract" xlink:to="aei20_CashFlowsFromOperatingActivitiesAbstract" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ProfitLoss" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProfitLoss"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_CashFlowsFromOperatingActivitiesAbstract" xlink:to="us-gaap_ProfitLoss" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_AdjustmentsToReconcileNetIncomeToNetCashProvidedByOperatingActivitiesAbstract" xlink:href="aei20-20220630.xsd#aei20_AdjustmentsToReconcileNetIncomeToNetCashProvidedByOperatingActivitiesAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_CashFlowsFromOperatingActivitiesAbstract" xlink:to="aei20_AdjustmentsToReconcileNetIncomeToNetCashProvidedByOperatingActivitiesAbstract" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_OtherDepreciationAndAmortization" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherDepreciationAndAmortization"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_AdjustmentsToReconcileNetIncomeToNetCashProvidedByOperatingActivitiesAbstract" xlink:to="us-gaap_OtherDepreciationAndAmortization" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ImpairmentOfRealEstate" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ImpairmentOfRealEstate"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_AdjustmentsToReconcileNetIncomeToNetCashProvidedByOperatingActivitiesAbstract" xlink:to="us-gaap_ImpairmentOfRealEstate" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_GainLossOnDispositionOfAssets1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GainLossOnDispositionOfAssets1"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_AdjustmentsToReconcileNetIncomeToNetCashProvidedByOperatingActivitiesAbstract" xlink:to="us-gaap_GainLossOnDispositionOfAssets1" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInReceivables" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInReceivables"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_AdjustmentsToReconcileNetIncomeToNetCashProvidedByOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInReceivables" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInAccountsPayableRelatedParties" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsPayableRelatedParties"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_AdjustmentsToReconcileNetIncomeToNetCashProvidedByOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInAccountsPayableRelatedParties" order="4" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivities" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivities"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_CashFlowsFromOperatingActivitiesAbstract" xlink:to="us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivities" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_NetCashProvidedByUsedInOperatingActivities" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivities" xlink:to="us-gaap_NetCashProvidedByUsedInOperatingActivities" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_CashFlowsFromInvestingActivitiesAbstract" xlink:href="aei20-20220630.xsd#aei20_CashFlowsFromInvestingActivitiesAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfCashFlowsAbstract" xlink:to="aei20_CashFlowsFromInvestingActivitiesAbstract" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromSaleOfRealEstateHeldforinvestment" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromSaleOfRealEstateHeldforinvestment"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_CashFlowsFromInvestingActivitiesAbstract" xlink:to="us-gaap_ProceedsFromSaleOfRealEstateHeldforinvestment" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_CashFlowsFromFinancingActivitiesAbstract" xlink:href="aei20-20220630.xsd#aei20_CashFlowsFromFinancingActivitiesAbstract"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfCashFlowsAbstract" xlink:to="aei20_CashFlowsFromFinancingActivitiesAbstract" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PaymentsOfCapitalDistribution" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsOfCapitalDistribution"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_CashFlowsFromFinancingActivitiesAbstract" xlink:to="us-gaap_PaymentsOfCapitalDistribution" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PaymentsForRepurchaseOfRedeemableNoncontrollingInterest" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsForRepurchaseOfRedeemableNoncontrollingInterest"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_CashFlowsFromFinancingActivitiesAbstract" xlink:to="us-gaap_PaymentsForRepurchaseOfRedeemableNoncontrollingInterest" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PaymentsForRepurchaseOfRedeemableNoncontrollingInterest" xlink:to="us-gaap_NetCashProvidedByUsedInFinancingActivities" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfCashFlowsAbstract" xlink:to="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementOfCashFlowsAbstract" xlink:to="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents" order="4" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/ShareholdersEquityType2or3">
<loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
<loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountAxis"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain_0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain_0" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_StatementEquityComponentsAxis" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_StatementEquityComponentsAxis" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_EquityComponentDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis" xlink:to="us-gaap_EquityComponentDomain" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_EquityComponentDomain_0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis" xlink:to="us-gaap_EquityComponentDomain_0" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_GeneralPartnersUnitsMember" xlink:href="aei20-20220630.xsd#aei20_GeneralPartnersUnitsMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="aei20_GeneralPartnersUnitsMember" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_LimitedPartnersUnitsMember" xlink:href="aei20-20220630.xsd#aei20_LimitedPartnersUnitsMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="aei20_LimitedPartnersUnitsMember" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_GeneralPartnerMember" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GeneralPartnerMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" xlink:to="us-gaap_GeneralPartnerMember" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LimitedPartnerMember" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LimitedPartnerMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" xlink:to="us-gaap_LimitedPartnerMember" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnersCapital" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnersCapital"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_PartnersCapital" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LimitedPartnersCapitalAccountUnitsOutstanding" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LimitedPartnersCapitalAccountUnitsOutstanding"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_LimitedPartnersCapitalAccountUnitsOutstanding" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_DistributionMadeToLimitedPartnerCashDistributionsDeclared" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DistributionMadeToLimitedPartnerCashDistributionsDeclared"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_DistributionMadeToLimitedPartnerCashDistributionsDeclared" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnersCapitalAccountRedemptions" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnersCapitalAccountRedemptions"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_PartnersCapitalAccountRedemptions" order="4" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnersCapitalAccountUnitsRedeemed" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnersCapitalAccountUnitsRedeemed"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_PartnersCapitalAccountUnitsRedeemed" order="5" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ProfitLoss" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProfitLoss"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_ProfitLoss" order="6" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/BasisofAccounting">
<loc xlink:type="locator" xlink:label="us-gaap_AccountingPoliciesAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountingPoliciesAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_BasisOfAccounting" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_BasisOfAccounting"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_BasisOfAccounting" order="0" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/Organization"> <!-- [WMV5][XcpuxYn59dz7vgYynUDuwG7r97K2jUTysl7vcz0lJt+s2JDua46hsF9zUBIOOX7+pla0tev4WgtIkqQSkVVumUJe3EE56QUfGThvvganhxhhKsS7enCQ1dJE7Bb4lywWKSA1/X/1VZ6O3soInECkfF2OJkvK8bVEjvK99R3lh3Gv5v2PNnMaJn4sMJ9042f4bpfEQRRJHbBN1HLJDlkWiJesUeytLJHt] CSR -->
<loc xlink:type="locator" xlink:label="us-gaap_AccountingPoliciesAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountingPoliciesAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_OrganizationConsolidationBasisOfPresentationBusinessDescriptionAndAccountingPoliciesTextBlock" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OrganizationConsolidationBasisOfPresentationBusinessDescriptionAndAccountingPoliciesTextBlock"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_OrganizationConsolidationBasisOfPresentationBusinessDescriptionAndAccountingPoliciesTextBlock" order="0" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/RecentlyIssuedAccountingPronouncements">
<loc xlink:type="locator" xlink:label="us-gaap_AccountingPoliciesAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountingPoliciesAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_NewAccountingPronouncementsPolicyPolicyTextBlock" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NewAccountingPronouncementsPolicyPolicyTextBlock"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_NewAccountingPronouncementsPolicyPolicyTextBlock" order="0" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/RealEstateInvestments">
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateDisclosureTextBlock" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateDisclosureTextBlock"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RealEstateAbstract" xlink:to="us-gaap_RealEstateDisclosureTextBlock" order="0" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/PayabletoAEIFundManagementInc">
<loc xlink:type="locator" xlink:label="us-gaap_PayablesAndAccrualsAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PayablesAndAccrualsAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_AccountsPayableAndAccruedLiabilitiesDisclosureTextBlock" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesDisclosureTextBlock"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PayablesAndAccrualsAbstract" xlink:to="us-gaap_AccountsPayableAndAccruedLiabilitiesDisclosureTextBlock" order="0" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/PartnersCapital">
<loc xlink:type="locator" xlink:label="us-gaap_PartnersCapitalNotesAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnersCapitalNotesAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnersCapitalNotesDisclosureTextBlock" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnersCapitalNotesDisclosureTextBlock"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnersCapitalNotesAbstract" xlink:to="us-gaap_PartnersCapitalNotesDisclosureTextBlock" order="0" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/FairValueMeasurements">
<loc xlink:type="locator" xlink:label="us-gaap_FairValueDisclosuresAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueDisclosuresAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_FairValueDisclosuresTextBlock" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueDisclosuresTextBlock"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueDisclosuresAbstract" xlink:to="us-gaap_FairValueDisclosuresTextBlock" order="0" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/AccountingPoliciesByPolicy">
<loc xlink:type="locator" xlink:label="us-gaap_AccountingPoliciesAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountingPoliciesAbstract"/>
<loc xlink:type="locator" xlink:label="us-gaap_KeyProvisionsOfOperatingOrPartnershipAgreementDescription" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_KeyProvisionsOfOperatingOrPartnershipAgreementDescription"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_KeyProvisionsOfOperatingOrPartnershipAgreementDescription" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_NewAccountingPronouncementOrChangeInAccountingPrincipleDescription" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NewAccountingPronouncementOrChangeInAccountingPrincipleDescription"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_NewAccountingPronouncementOrChangeInAccountingPrincipleDescription" order="1" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/RealEstateInvestmentsDetails">
<loc xlink:type="locator" xlink:label="aei20_RealEstateInvestmentsDetailsLineItems" xlink:href="aei20-20220630.xsd#aei20_RealEstateInvestmentsDetailsLineItems"/>
<loc xlink:type="locator" xlink:label="aei20_RealEstateInvestmentsDetailsTable" xlink:href="aei20-20220630.xsd#aei20_RealEstateInvestmentsDetailsTable"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="aei20_RealEstateInvestmentsDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="aei20_RealEstateInvestmentsDetailsTable" xlink:to="us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis" xlink:to="us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain_0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis" xlink:to="us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain_0" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="aei20_RealEstateInvestmentsDetailsTable" xlink:to="srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis" xlink:to="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_0" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis" xlink:to="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_0" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_FreseniusMedicalCenterGreenOHMember" xlink:href="aei20-20220630.xsd#aei20_FreseniusMedicalCenterGreenOHMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain" xlink:to="aei20_FreseniusMedicalCenterGreenOHMember" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_BassetFurnitureFredericksburgVAMember" xlink:href="aei20-20220630.xsd#aei20_BassetFurnitureFredericksburgVAMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain" xlink:to="aei20_BassetFurnitureFredericksburgVAMember" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_StaplesVernonHillsILMember" xlink:href="aei20-20220630.xsd#aei20_StaplesVernonHillsILMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain" xlink:to="aei20_StaplesVernonHillsILMember" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_JaredJewelryHanoverMDMember" xlink:href="aei20-20220630.xsd#aei20_JaredJewelryHanoverMDMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain" xlink:to="aei20_JaredJewelryHanoverMDMember" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_FamilyDollarMobileALMember" xlink:href="aei20-20220630.xsd#aei20_FamilyDollarMobileALMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain" xlink:to="aei20_FamilyDollarMobileALMember" order="4" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_DollarTreeIndianapolisINMember" xlink:href="aei20-20220630.xsd#aei20_DollarTreeIndianapolisINMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain" xlink:to="aei20_DollarTreeIndianapolisINMember" order="0" xbrldt:closed="true"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain" xlink:to="aei20_JaredJewelryHanoverMDMember" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_DisposalDate1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DisposalDate1"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_DisposalDate1" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromSaleOfRealEstate" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromSaleOfRealEstate"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_ProceedsFromSaleOfRealEstate" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_GainLossOnDispositionOfAssets1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GainLossOnDispositionOfAssets1"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_GainLossOnDispositionOfAssets1" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateCostOfRealEstateSold" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateCostOfRealEstateSold"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_RealEstateCostOfRealEstateSold" order="4" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateAccumulatedDepreciation" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateAccumulatedDepreciation"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_RealEstateAccumulatedDepreciation" order="5" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateAccumulatedDepreciationRealEstateSold" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateAccumulatedDepreciationRealEstateSold"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_RealEstateAccumulatedDepreciationRealEstateSold" order="6" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ImpairmentOfRealEstate" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ImpairmentOfRealEstate"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_ImpairmentOfRealEstate" order="7" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentGross" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_PropertyPlantAndEquipmentGross" order="8" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentFairValueDisclosure" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentFairValueDisclosure"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_PropertyPlantAndEquipmentFairValueDisclosure" order="9" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromSaleOfOtherRealEstate" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromSaleOfOtherRealEstate"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_ProceedsFromSaleOfOtherRealEstate" order="10" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_RealEstateHeldforsale" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateHeldforsale"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_RealEstateHeldforsale" order="11" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LandAvailableForSale" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LandAvailableForSale"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_RealEstateInvestmentsDetailsLineItems" xlink:to="us-gaap_LandAvailableForSale" order="12" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/PartnersCapitalDetails">
<loc xlink:type="locator" xlink:label="aei20_PartnersCapitalDetailsLineItems" xlink:href="aei20-20220630.xsd#aei20_PartnersCapitalDetailsLineItems"/>
<loc xlink:type="locator" xlink:label="aei20_PartnersCapitalDetailsTable" xlink:href="aei20-20220630.xsd#aei20_PartnersCapitalDetailsTable"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="aei20_PartnersCapitalDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountAxis"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="aei20_PartnersCapitalDetailsTable" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain_0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountAxis" xlink:to="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain_0" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_LimitedPartnerMember" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LimitedPartnerMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" xlink:to="us-gaap_LimitedPartnerMember" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_GeneralPartnerMember" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GeneralPartnerMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PartnerTypeOfPartnersCapitalAccountNameDomain" xlink:to="us-gaap_GeneralPartnerMember" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_DistributionMadeToLimitedPartnerCashDistributionsDeclared" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DistributionMadeToLimitedPartnerCashDistributionsDeclared"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="us-gaap_DistributionMadeToLimitedPartnerCashDistributionsDeclared" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_DistributionMadeToLimitedPartnerDistributionsDeclaredPerUnit" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DistributionMadeToLimitedPartnerDistributionsDeclaredPerUnit"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="us-gaap_DistributionMadeToLimitedPartnerDistributionsDeclaredPerUnit" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_WeightedAverageLimitedPartnershipUnitsOutstanding" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageLimitedPartnershipUnitsOutstanding"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="us-gaap_WeightedAverageLimitedPartnershipUnitsOutstanding" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_DistributionsPerUnitOfNetIncome" xlink:href="aei20-20220630.xsd#aei20_DistributionsPerUnitOfNetIncome"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="aei20_DistributionsPerUnitOfNetIncome" order="4" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_DistributionsPerUnitOfReturnOfCapital" xlink:href="aei20-20220630.xsd#aei20_DistributionsPerUnitOfReturnOfCapital"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="aei20_DistributionsPerUnitOfReturnOfCapital" order="5" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_SaleProceedsDistributionMadeToMemberOrLimitedPartner" xlink:href="aei20-20220630.xsd#aei20_SaleProceedsDistributionMadeToMemberOrLimitedPartner"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="aei20_SaleProceedsDistributionMadeToMemberOrLimitedPartner" order="6" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_UnitsOfPartnershipInterestDescription" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_UnitsOfPartnershipInterestDescription"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="us-gaap_UnitsOfPartnershipInterestDescription" order="7" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PartnersCapitalLimitationOfLiability" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PartnersCapitalLimitationOfLiability"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="us-gaap_PartnersCapitalLimitationOfLiability" order="8" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="aei20_SaleProceedsDistributionMadetoLimitedPartnerPerUnit" xlink:href="aei20-20220630.xsd#aei20_SaleProceedsDistributionMadetoLimitedPartnerPerUnit"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_PartnersCapitalDetailsLineItems" xlink:to="aei20_SaleProceedsDistributionMadetoLimitedPartnerPerUnit" order="9" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/FairValueMeasurementsDetails">
<loc xlink:type="locator" xlink:label="aei20_FairValueMeasurementsDetailsLineItems" xlink:href="aei20-20220630.xsd#aei20_FairValueMeasurementsDetailsLineItems"/>
<loc xlink:type="locator" xlink:label="aei20_FairValueMeasurementsDetailsTable" xlink:href="aei20-20220630.xsd#aei20_FairValueMeasurementsDetailsTable"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="aei20_FairValueMeasurementsDetailsLineItems" xlink:to="aei20_FairValueMeasurementsDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_FairValueByMeasurementBasisAxis" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="aei20_FairValueMeasurementsDetailsTable" xlink:to="us-gaap_FairValueByMeasurementBasisAxis" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_FairValueDisclosureItemAmountsDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FairValueByMeasurementBasisAxis" xlink:to="us-gaap_FairValueDisclosureItemAmountsDomain" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PortionAtFairValueFairValueDisclosureMember" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FairValueByMeasurementBasisAxis" xlink:to="us-gaap_PortionAtFairValueFairValueDisclosureMember" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="aei20_FairValueMeasurementsDetailsTable" xlink:to="us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis" xlink:to="us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis" xlink:to="us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_0" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_EstimateOfFairValueFairValueDisclosureMember" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueDisclosureItemAmountsDomain" xlink:to="us-gaap_EstimateOfFairValueFairValueDisclosureMember" order="0" xbrldt:closed="true"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueDisclosureItemAmountsDomain" xlink:to="us-gaap_PortionAtFairValueFairValueDisclosureMember" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ReserveForImpairmentOfIntangibleAssetsMember" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReserveForImpairmentOfIntangibleAssetsMember"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain" xlink:to="us-gaap_ReserveForImpairmentOfIntangibleAssetsMember" order="0" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentFairValueDisclosure" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentFairValueDisclosure"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_FairValueMeasurementsDetailsLineItems" xlink:to="us-gaap_PropertyPlantAndEquipmentFairValueDisclosure" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="us-gaap_ImpairmentOfRealEstate" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ImpairmentOfRealEstate"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="aei20_FairValueMeasurementsDetailsLineItems" xlink:to="us-gaap_ImpairmentOfRealEstate" order="2" xbrldt:closed="true"/>
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://aeifunds.com/role/DocumentAndEntityInformation">
<loc xlink:type="locator" xlink:label="dei_DocumentInformationLineItems" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_DocumentInformationLineItems"/>
<loc xlink:type="locator" xlink:label="dei_DocumentInformationTable" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_DocumentInformationTable"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentInformationTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityRegistrantName" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityRegistrantName"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityRegistrantName" order="1" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_DocumentType" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_DocumentType"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentType" order="2" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_CurrentFiscalYearEndDate" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_CurrentFiscalYearEndDate"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_CurrentFiscalYearEndDate" order="3" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityCommonStockSharesOutstanding" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityCommonStockSharesOutstanding"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityCommonStockSharesOutstanding" order="4" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_AmendmentFlag" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_AmendmentFlag"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_AmendmentFlag" order="5" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityCentralIndexKey" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityCentralIndexKey"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityCentralIndexKey" order="6" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityCurrentReportingStatus" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityCurrentReportingStatus"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityCurrentReportingStatus" order="7" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityFilerCategory" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityFilerCategory"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityFilerCategory" order="8" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_DocumentPeriodEndDate" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_DocumentPeriodEndDate"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentPeriodEndDate" order="9" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_DocumentFiscalYearFocus" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_DocumentFiscalYearFocus"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentFiscalYearFocus" order="10" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_DocumentFiscalPeriodFocus" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_DocumentFiscalPeriodFocus"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentFiscalPeriodFocus" order="11" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntitySmallBusiness" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntitySmallBusiness"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntitySmallBusiness" order="12" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityEmergingGrowthCompany" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityEmergingGrowthCompany"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityEmergingGrowthCompany" order="13" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityShellCompany" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityShellCompany"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityShellCompany" order="14" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_DocumentQuarterlyReport" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_DocumentQuarterlyReport"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentQuarterlyReport" order="15" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityFileNumber" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityFileNumber"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityFileNumber" order="16" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityIncorporationStateCountryCode" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityIncorporationStateCountryCode"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityIncorporationStateCountryCode" order="17" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityAddressStateOrProvince" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityAddressStateOrProvince"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityAddressStateOrProvince" order="18" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityTaxIdentificationNumber" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityTaxIdentificationNumber"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityTaxIdentificationNumber" order="19" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityAddressAddressLine1" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityAddressAddressLine1"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityAddressAddressLine1" order="20" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityAddressCityOrTown" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityAddressCityOrTown"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityAddressCityOrTown" order="21" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityAddressPostalZipCode" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityAddressPostalZipCode"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityAddressPostalZipCode" order="22" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_CityAreaCode" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_CityAreaCode"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_CityAreaCode" order="23" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_LocalPhoneNumber" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_LocalPhoneNumber"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_LocalPhoneNumber" order="24" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityInformationFormerLegalOrRegisteredName" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityInformationFormerLegalOrRegisteredName"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityInformationFormerLegalOrRegisteredName" order="25" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_SecurityExchangeName" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_SecurityExchangeName"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_SecurityExchangeName" order="26" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_NoTradingSymbolFlag" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_NoTradingSymbolFlag"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_NoTradingSymbolFlag" order="27" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_Security12gTitle" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_Security12gTitle"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_Security12gTitle" order="28" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_EntityInteractiveDataCurrent" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityInteractiveDataCurrent"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityInteractiveDataCurrent" order="29" xbrldt:closed="true"/>
<loc xlink:type="locator" xlink:label="dei_DocumentTransitionReport" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_DocumentTransitionReport"/>
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentTransitionReport" order="30" xbrldt:closed="true"/>
</definitionLink>
</linkbase>

↑Top
Filing Submission 0000894245-22-000098   –   Alternative Formats (Word / Rich Text, HTML, Plain Text, et al.)
Copyright © 2024 Fran Finnegan & Company LLC – All Rights Reserved.
About — Privacy — Redactions — Help — Sun., Apr. 28, 4:14:25.1am ET