SEC Info  
    Home      Search      My Interests      Help      Sign In      Please Sign In

Escalon Medical Corp. – ‘10-K’ for 6/30/22 – ‘EX-101.DEF’

On:  Wednesday, 9/28/22, at 4:12pm ET   ·   For:  6/30/22   ·   Accession #:  862668-22-23   ·   File #:  0-20127

Previous ‘10-K’:  ‘10-K/A’ on 10/22/21 for 6/30/21   ·   Next & Latest:  ‘10-K’ on 10/13/23 for 6/30/23

Find Words in Filings emoji
 
  in    Show  and   Hints

  As Of               Filer                 Filing    For·On·As Docs:Size

 9/28/22  Escalon Medical Corp.             10-K        6/30/22   75:6.5M

Annual Report   —   Form 10-K

Filing Table of Contents

Document/Exhibit                   Description                      Pages   Size 

 1: 10-K        Annual Report                                       HTML   1.34M 
 2: EX-23.1     Consent of Expert or Counsel                        HTML     20K 
 3: EX-31.1     Certification -- §302 - SOA'02                      HTML     24K 
 4: EX-31.2     Certification -- §302 - SOA'02                      HTML     24K 
 5: EX-32.1     Certification -- §906 - SOA'02                      HTML     21K 
 6: EX-32.2     Certification -- §906 - SOA'02                      HTML     21K 
12: R1          Cover                                               HTML     51K 
13: R2          Document And Entity Information                     HTML     66K 
14: R3          Audit Information                                   HTML     25K 
15: R4          Consolidated Balance Sheets                         HTML    143K 
16: R5          Consolidated Balance Sheets (Parenthetical)         HTML     35K 
17: R6          Consolidated Statements Of Operations               HTML     95K 
18: R7          Consolidated Statements Of Shareholders' Equity     HTML     45K 
                and Comprehensive Loss                                           
19: R8          Consolidated Statements Of Cash Flows               HTML     89K 
20: R9          Consolidated Statements Of Cash Flows               HTML     41K 
                (Parenthetical)                                                  
21: R10         Organization and Description of Business            HTML     26K 
22: R11         Going concern (Notes)                               HTML     25K 
23: R12         Significant Accounting Policies                     HTML    115K 
24: R13         Intangible assets (Notes)                           HTML     42K 
25: R14         Accrued expense (Notes)                             HTML     43K 
26: R15         Line of credit (Notes)                              HTML     26K 
27: R16         Long-term Debt (Notes)                              HTML     29K 
28: R17         Capital Stock Transactions                          HTML     55K 
29: R18         Income Taxes                                        HTML     65K 
30: R19         Commitments and Contingencies                       HTML     23K 
31: R20         Retirement and post retirement plans (Notes)        HTML     29K 
32: R21         Related Party Transactions                          HTML     27K 
33: R22         Concentration of credit risk (Notes)                HTML     37K 
34: R23         Leases (Notes)                                      HTML     53K 
35: R24         Significant Accounting Policies (Policies)          HTML    153K 
36: R25         Significant Accounting Policies (Tables)            HTML     86K 
37: R26         Significant Accounting Policies Property Plant and  HTML     31K 
                Equipment (Tables)                                               
38: R27         Significant Accounting Policies Deferred revenue    HTML     29K 
                (Tables)                                                         
39: R28         Intangible assets (Tables)                          HTML     52K 
40: R29         Accrued expense (Tables)                            HTML     31K 
41: R30         Long-term Debt EIDL maturities (Tables)             HTML     27K 
42: R31         Income Taxes (Tables)                               HTML     61K 
43: R32         Retirement and post retirement plans (Tables)       HTML     29K 
44: R33         Concentration of credit risk (Tables)               HTML     34K 
45: R34         Leases (Tables)                                     HTML     44K 
46: R35         Organization and Description of Business (Details)  HTML     21K 
47: R36         Going concern (Details)                             HTML     23K 
48: R37         Significant Accounting Policies (Cash and Cash      HTML     24K 
                Equivalents) (Details)                                           
49: R38         Significant Accounting Policies (Inventory)         HTML     33K 
                (Details)                                                        
50: R39         Significant Accounting Policies (Accounts           HTML     27K 
                Receivable) (Details)                                            
51: R40         Significant Accounting Policies (Net Income (loss)  HTML     40K 
                Per Share) (Details)                                             
52: R41         Significant Accounting Policies Earning per share   HTML     71K 
                details (Details)                                                
53: R42         Significant Accounting Policies Property pant and   HTML     37K 
                equipment (Details)                                              
54: R43         Significant Accounting Policies advertising         HTML     21K 
                expense (Details)                                                
55: R44         Significant Accounting Policies deferred revenue    HTML     28K 
                (Details)                                                        
56: R45         Intangible assets (Details)                         HTML     43K 
57: R46         Accrued expense (Details)                           HTML     36K 
58: R47         Line of credit (Details)                            HTML     40K 
59: R48         Long-term Debt (Details)                            HTML     47K 
60: R49         Capital Stock Transactions (Stock Option Plans)     HTML     58K 
                (Narrative) (Details)                                            
61: R50         Capital Stock Transactions (Stock Option Activity)  HTML     61K 
                (Details)                                                        
62: R51         Income Taxes (Provision for Income Taxes)           HTML     27K 
                (Details)                                                        
63: R52         Income Taxes (Effective Income Tax Rate) (Details)  HTML     30K 
64: R53         Income Taxes (Components of Net Deferred Income     HTML     57K 
                Tax Assets and Liabilities) (Details)                            
65: R54         Income Taxes Operation loss carry forward           HTML     48K 
                (Details)                                                        
66: R55         Retirement and post retirement plans (Details)      HTML     27K 
67: R56         Retirement and post retirement plans - liability    HTML     27K 
                rollforward (Details)                                            
68: R57         Related Party Transactions (Details)                HTML     38K 
69: R58         Concentration of credit risk (Details)              HTML     79K 
70: R59         Leases (Details)                                    HTML     49K 
73: XML         IDEA XML File -- Filing Summary                      XML    133K 
71: XML         XBRL Instance -- esmc-20220630_htm                   XML   1.28M 
72: EXCEL       IDEA Workbook of Financial Reports                  XLSX    117K 
 8: EX-101.CAL  XBRL Calculations -- esmc-20220630_cal               XML    119K 
 9: EX-101.DEF  XBRL Definitions -- esmc-20220630_def                XML    596K 
10: EX-101.LAB  XBRL Labels -- esmc-20220630_lab                     XML   1.45M 
11: EX-101.PRE  XBRL Presentations -- esmc-20220630_pre              XML    930K 
 7: EX-101.SCH  XBRL Schema -- esmc-20220630                         XSD    130K 
74: JSON        XBRL Instance as JSON Data -- MetaLinks              371±   558K 
75: ZIP         XBRL Zipped Folder -- 0000862668-22-000023-xbrl      Zip    361K 


‘EX-101.DEF’   —   XBRL Definitions — esmc-20220630_def


This Exhibit is an XBRL XML File.


                                                                                                                                                                                
<?xml version="1.0" encoding="windows-1252"?>
<!-- XBRL Document Created with Wdesk from Workiva -->
<!-- Copyright 2022 Workiva -->
<!-- r:98ce2cd9-8642-4a0b-a5ce-da8e59984557,g:9f440f39-3670-4990-9b18-5ad31fb583c0 -->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
<link:roleRef roleURI="http://www.escalonmed.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="esmc-20220630.xsd#ConsolidatedBalanceSheets"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/ConsolidatedBalanceSheets" xlink:type="extended" id="i884c5d9db1734463bb1dc388a62905c9_ConsolidatedBalanceSheets">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_7e345a40-07b2-48cf-941d-27f583f31b4e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_8ab93fca-7220-42d0-85bd-aeb1e7ffb571" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_7e345a40-07b2-48cf-941d-27f583f31b4e" xlink:to="loc_us-gaap_AssetsAbstract_8ab93fca-7220-42d0-85bd-aeb1e7ffb571" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrentAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_8ab93fca-7220-42d0-85bd-aeb1e7ffb571" xlink:to="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_9f60c456-8e8a-498d-8f28-2e8e5a8a317f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_9f60c456-8e8a-498d-8f28-2e8e5a8a317f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashCurrent_ebf08a0b-722c-4cfa-85bf-b884bb3119a0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RestrictedCashCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_RestrictedCashCurrent_ebf08a0b-722c-4cfa-85bf-b884bb3119a0" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_f58014ce-064c-47d3-833a-b4a7ba83002b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsReceivableNetCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_f58014ce-064c-47d3-833a-b4a7ba83002b" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_1b90d780-f38b-4346-bd66-b03d14bf4890" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryNet"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_InventoryNet_1b90d780-f38b-4346-bd66-b03d14bf4890" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsCurrent_d58505f3-140e-41ac-9d96-d1fc1d7fe3ac" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_OtherAssetsCurrent_d58505f3-140e-41ac-9d96-d1fc1d7fe3ac" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_345ae809-7401-4911-b16c-4322705d2b94" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_AssetsCurrent_345ae809-7401-4911-b16c-4322705d2b94" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_f7de901b-0254-4506-845d-b28d79425632" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_f7de901b-0254-4506-845d-b28d79425632" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_e7db0bfc-522e-4761-a693-6098eb64bf5b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_e7db0bfc-522e-4761-a693-6098eb64bf5b" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_cb75ee35-4083-4cc9-a606-61be9a5b689a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_cb75ee35-4083-4cc9-a606-61be9a5b689a" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositsAssetsNoncurrent_6792928d-79e5-476a-ad13-1e20a29fcd29" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepositsAssetsNoncurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_DepositsAssetsNoncurrent_6792928d-79e5-476a-ad13-1e20a29fcd29" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_9895512e-8912-4465-a2f7-eab1b9b32ad1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Assets"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_39a963ab-989b-47c2-a29f-05aea2993f00" xlink:to="loc_us-gaap_Assets_9895512e-8912-4465-a2f7-eab1b9b32ad1" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesAndStockholdersEquityAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_7e345a40-07b2-48cf-941d-27f583f31b4e" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrentAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermBorrowings_ffdcf1fa-3fbb-4f65-b4b2-a545bdd12e9c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShortTermBorrowings"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_ShortTermBorrowings_ffdcf1fa-3fbb-4f65-b4b2-a545bdd12e9c" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableToBankCurrent_98ed5f5d-315e-407b-ae44-985ae9491822" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NotesPayableToBankCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_NotesPayableToBankCurrent_98ed5f5d-315e-407b-ae44-985ae9491822" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNotesPayableCurrent_470d722d-85fc-4fd4-a288-b804a931ead6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherNotesPayableCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_OtherNotesPayableCurrent_470d722d-85fc-4fd4-a288-b804a931ead6" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtCurrent_f1e5945a-9045-40a7-901f-153166b10ea1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SecuredDebtCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_SecuredDebtCurrent_f1e5945a-9045-40a7-901f-153166b10ea1" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_6be7b48d-8078-4942-bb56-288e70ab0105" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsPayableCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_AccountsPayableCurrent_6be7b48d-8078-4942-bb56-288e70ab0105" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_91abe5a0-2c85-4339-b243-c4827401798e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_91abe5a0-2c85-4339-b243-c4827401798e" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DueToRelatedPartiesCurrent_1fe070c5-8e51-4fc8-a2c3-4bc8ee9d991d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DueToRelatedPartiesCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_DueToRelatedPartiesCurrent_1fe070c5-8e51-4fc8-a2c3-4bc8ee9d991d" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_2dbe40ec-5a18-4a14-b281-67dfa890ce44" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_2dbe40ec-5a18-4a14-b281-67dfa890ce44" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRevenueCurrent_c7700cde-f45e-4430-83d3-5453b71ecfa1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredRevenueCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_DeferredRevenueCurrent_c7700cde-f45e-4430-83d3-5453b71ecfa1" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent_124fbcca-7196-4273-80a5-ef72dd266646" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent_124fbcca-7196-4273-80a5-ef72dd266646" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_e59c008e-0dc2-4921-9499-817ec463c7d3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_e645bd32-06bb-4037-8280-c9d76a003c23" xlink:to="loc_us-gaap_LiabilitiesCurrent_e59c008e-0dc2-4921-9499-817ec463c7d3" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLongTermNotesPayable_9d15fdfa-d356-4413-bd38-72b17d98c289" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherLongTermNotesPayable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_OtherLongTermNotesPayable_9d15fdfa-d356-4413-bd38-72b17d98c289" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_dc5317bc-8292-4da4-8af2-34134a02dbd6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_dc5317bc-8292-4da4-8af2-34134a02dbd6" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredLongTermDebt_d6e6605e-e356-4d64-8e2f-8fa055a38ed6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SecuredLongTermDebt"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_SecuredLongTermDebt_d6e6605e-e356-4d64-8e2f-8fa055a38ed6" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermNotesPayable_7caa2b3f-7623-42d6-a373-445a4a3bf57e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermNotesPayable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_LongTermNotesPayable_7caa2b3f-7623-42d6-a373-445a4a3bf57e" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrentStatementOfFinancialPositionExtensibleList_12a69ec6-c547-4009-a2a0-62d615dacd90" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityNoncurrentStatementOfFinancialPositionExtensibleList"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrentStatementOfFinancialPositionExtensibleList_12a69ec6-c547-4009-a2a0-62d615dacd90" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_9bb7fdde-6491-4e34-96fd-096dfc4bad2c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_9bb7fdde-6491-4e34-96fd-096dfc4bad2c" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesNoncurrent_36664931-dffa-4078-84b3-8fa864bb3320" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesNoncurrent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_LiabilitiesNoncurrent_36664931-dffa-4078-84b3-8fa864bb3320" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_99e3d53b-b093-4876-91cf-cf86215d64ec" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_Liabilities_99e3d53b-b093-4876-91cf-cf86215d64ec" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityAbstract_697792d8-a909-43bc-a4b7-fefda27e7b67" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquityAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_StockholdersEquityAbstract_697792d8-a909-43bc-a4b7-fefda27e7b67" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_6e11b438-3b42-463e-b2f1-5c003524d824" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PreferredStockValue"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_697792d8-a909-43bc-a4b7-fefda27e7b67" xlink:to="loc_us-gaap_PreferredStockValue_6e11b438-3b42-463e-b2f1-5c003524d824" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_2f56ea90-e344-447c-aca7-ccae0064748c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockValue"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_697792d8-a909-43bc-a4b7-fefda27e7b67" xlink:to="loc_us-gaap_CommonStockValue_2f56ea90-e344-447c-aca7-ccae0064748c" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_ebbfecd6-66d1-4209-b314-da075f7ad03e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapital"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_697792d8-a909-43bc-a4b7-fefda27e7b67" xlink:to="loc_us-gaap_AdditionalPaidInCapital_ebbfecd6-66d1-4209-b314-da075f7ad03e" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_d5033ead-a5cc-4298-8f0a-73d3d0c32262" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_697792d8-a909-43bc-a4b7-fefda27e7b67" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_d5033ead-a5cc-4298-8f0a-73d3d0c32262" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_3c9c5abb-c963-4ad7-8ed8-bb49255e7eb8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_697792d8-a909-43bc-a4b7-fefda27e7b67" xlink:to="loc_us-gaap_StockholdersEquity_3c9c5abb-c963-4ad7-8ed8-bb49255e7eb8" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_ee0ae05f-2b8c-4e8a-8901-d1ab378b4b4d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f0866226-1704-4696-8712-fd11c451019c" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquity_ee0ae05f-2b8c-4e8a-8901-d1ab378b4b4d" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_dcf6c8c1-6bf0-4897-b6dc-8085516c211b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_7e345a40-07b2-48cf-941d-27f583f31b4e" xlink:to="loc_us-gaap_StatementTable_dcf6c8c1-6bf0-4897-b6dc-8085516c211b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermDebtTypeAxis_6d9f90af-3f1e-4d40-8ff4-7c1a2735315a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShortTermDebtTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_dcf6c8c1-6bf0-4897-b6dc-8085516c211b" xlink:to="loc_us-gaap_ShortTermDebtTypeAxis_6d9f90af-3f1e-4d40-8ff4-7c1a2735315a" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermDebtTypeDomain_6d9f90af-3f1e-4d40-8ff4-7c1a2735315a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShortTermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShortTermDebtTypeAxis_6d9f90af-3f1e-4d40-8ff4-7c1a2735315a" xlink:to="loc_us-gaap_ShortTermDebtTypeDomain_6d9f90af-3f1e-4d40-8ff4-7c1a2735315a_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermDebtTypeDomain_f68df6f1-4f5b-4c4a-938f-713060ee1d87" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShortTermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShortTermDebtTypeAxis_6d9f90af-3f1e-4d40-8ff4-7c1a2735315a" xlink:to="loc_us-gaap_ShortTermDebtTypeDomain_f68df6f1-4f5b-4c4a-938f-713060ee1d87" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_RelatedPartyMember_2119f5d5-adf2-405f-abc2-e249c034a018" xlink:href="esmc-20220630.xsd#esmc_RelatedPartyMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShortTermDebtTypeDomain_f68df6f1-4f5b-4c4a-938f-713060ee1d87" xlink:to="loc_esmc_RelatedPartyMember_2119f5d5-adf2-405f-abc2-e249c034a018" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_0f2b706b-60e2-4244-840e-cf5ecb088f47" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_dcf6c8c1-6bf0-4897-b6dc-8085516c211b" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_0f2b706b-60e2-4244-840e-cf5ecb088f47" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_0f2b706b-60e2-4244-840e-cf5ecb088f47_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_0f2b706b-60e2-4244-840e-cf5ecb088f47" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_0f2b706b-60e2-4244-840e-cf5ecb088f47_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_006084a9-c186-474a-bdf3-908408cadf4a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_0f2b706b-60e2-4244-840e-cf5ecb088f47" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_006084a9-c186-474a-bdf3-908408cadf4a" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_PayrollProtectionProgramLoanMember_39fc2b8e-8f69-433b-aff1-8a02febe1540" xlink:href="esmc-20220630.xsd#esmc_PayrollProtectionProgramLoanMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_006084a9-c186-474a-bdf3-908408cadf4a" xlink:to="loc_esmc_PayrollProtectionProgramLoanMember_39fc2b8e-8f69-433b-aff1-8a02febe1540" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_fa949d84-b0bd-4538-8229-b934bf62580f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SecuredDebtMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_006084a9-c186-474a-bdf3-908408cadf4a" xlink:to="loc_us-gaap_SecuredDebtMember_fa949d84-b0bd-4538-8229-b934bf62580f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_SmallBusinessAssociationLoanMember_7c3b8cf1-fc1a-4a1a-8782-ed02ffc74655" xlink:href="esmc-20220630.xsd#esmc_SmallBusinessAssociationLoanMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_006084a9-c186-474a-bdf3-908408cadf4a" xlink:to="loc_esmc_SmallBusinessAssociationLoanMember_7c3b8cf1-fc1a-4a1a-8782-ed02ffc74655" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_a8bffbb3-baf0-47e2-b311-b2c1efb199bd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_dcf6c8c1-6bf0-4897-b6dc-8085516c211b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_a8bffbb3-baf0-47e2-b311-b2c1efb199bd" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_a8bffbb3-baf0-47e2-b311-b2c1efb199bd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_a8bffbb3-baf0-47e2-b311-b2c1efb199bd" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_a8bffbb3-baf0-47e2-b311-b2c1efb199bd_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_c0853382-2a92-4bb6-b74a-1359f16309d6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_a8bffbb3-baf0-47e2-b311-b2c1efb199bd" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_c0853382-2a92-4bb6-b74a-1359f16309d6" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/ConsolidatedStatementsOfOperations" xlink:type="simple" xlink:href="esmc-20220630.xsd#ConsolidatedStatementsOfOperations"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/ConsolidatedStatementsOfOperations" xlink:type="extended" id="i4a932f322a784dd7b1476e5b82aaecf8_ConsolidatedStatementsOfOperations">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EntityWideInformationRevenueFromExternalCustomerLineItems_4519d837-4867-4fb1-bbc5-cd18b7a2e857" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EntityWideInformationRevenueFromExternalCustomerLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerIncludingAssessedTax_ee9662fb-bfef-4dc1-8067-8ef621add291" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenueFromContractWithCustomerIncludingAssessedTax"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EntityWideInformationRevenueFromExternalCustomerLineItems_4519d837-4867-4fb1-bbc5-cd18b7a2e857" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerIncludingAssessedTax_ee9662fb-bfef-4dc1-8067-8ef621add291" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_01339f68-0548-488a-9072-7dbda8734477" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EntityWideInformationRevenueFromExternalCustomerLineItems_4519d837-4867-4fb1-bbc5-cd18b7a2e857" xlink:to="loc_us-gaap_NetIncomeLoss_01339f68-0548-488a-9072-7dbda8734477" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEntityWideInformationRevenueFromExternalCustomersByProductsAndServicesTable_44f32ede-ba3b-4041-95a7-89d5c1cea4b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfEntityWideInformationRevenueFromExternalCustomersByProductsAndServicesTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EntityWideInformationRevenueFromExternalCustomerLineItems_4519d837-4867-4fb1-bbc5-cd18b7a2e857" xlink:to="loc_us-gaap_ScheduleOfEntityWideInformationRevenueFromExternalCustomersByProductsAndServicesTable_44f32ede-ba3b-4041-95a7-89d5c1cea4b4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_023f5277-279a-4880-875b-305d3c9ef511" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ProductOrServiceAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEntityWideInformationRevenueFromExternalCustomersByProductsAndServicesTable_44f32ede-ba3b-4041-95a7-89d5c1cea4b4" xlink:to="loc_srt_ProductOrServiceAxis_023f5277-279a-4880-875b-305d3c9ef511" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_023f5277-279a-4880-875b-305d3c9ef511_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ProductsAndServicesDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_023f5277-279a-4880-875b-305d3c9ef511" xlink:to="loc_srt_ProductsAndServicesDomain_023f5277-279a-4880-875b-305d3c9ef511_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_8f2cde26-42e6-43af-b130-23c07f6d6005" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ProductsAndServicesDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_023f5277-279a-4880-875b-305d3c9ef511" xlink:to="loc_srt_ProductsAndServicesDomain_8f2cde26-42e6-43af-b130-23c07f6d6005" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductMember_d64b18f8-b3f8-47fe-96dd-32b07b73bd23" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProductMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_8f2cde26-42e6-43af-b130-23c07f6d6005" xlink:to="loc_us-gaap_ProductMember_d64b18f8-b3f8-47fe-96dd-32b07b73bd23" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServiceMember_31b9734b-535a-46ac-bad0-e58f934767b0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ServiceMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_8f2cde26-42e6-43af-b130-23c07f6d6005" xlink:to="loc_us-gaap_ServiceMember_31b9734b-535a-46ac-bad0-e58f934767b0" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/ConsolidatedStatementsOfShareholdersEquityandComprehensiveLoss" xlink:type="simple" xlink:href="esmc-20220630.xsd#ConsolidatedStatementsOfShareholdersEquityandComprehensiveLoss"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/ConsolidatedStatementsOfShareholdersEquityandComprehensiveLoss" xlink:type="extended" id="i8650b0d9477248ef8f78b6f18868a3f6_ConsolidatedStatementsOfShareholdersEquityandComprehensiveLoss">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_8454e923-f7ad-4236-aba7-580ab79ab0ed" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_5847767a-f4d4-460d-9127-9c94b5b2cbed" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PreferredStockSharesOutstanding"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_8454e923-f7ad-4236-aba7-580ab79ab0ed" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_5847767a-f4d4-460d-9127-9c94b5b2cbed" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_6f56dcca-96dd-4f8c-9115-4c4683c77da0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PreferredStockValue"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_8454e923-f7ad-4236-aba7-580ab79ab0ed" xlink:to="loc_us-gaap_PreferredStockValue_6f56dcca-96dd-4f8c-9115-4c4683c77da0" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_3c75701b-4180-4791-8f3c-9893a6651e0f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_8454e923-f7ad-4236-aba7-580ab79ab0ed" xlink:to="loc_us-gaap_StockholdersEquity_3c75701b-4180-4791-8f3c-9893a6651e0f" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_77a07517-a7ed-40c5-863b-22203fccf4bf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharesOutstanding"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_8454e923-f7ad-4236-aba7-580ab79ab0ed" xlink:to="loc_us-gaap_SharesOutstanding_77a07517-a7ed-40c5-863b-22203fccf4bf" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_70abc12b-e9e0-4be7-8a29-c475ed688513" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_8454e923-f7ad-4236-aba7-580ab79ab0ed" xlink:to="loc_us-gaap_NetIncomeLoss_70abc12b-e9e0-4be7-8a29-c475ed688513" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_5e66ee23-66fc-4d06-978d-97e2a42c33ab" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_64f7574f-12cd-435d-9077-76cce364a363" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharesOutstanding"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_f8f41d17-d301-4373-a3b5-6cdec834c6a6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_8454e923-f7ad-4236-aba7-580ab79ab0ed" xlink:to="loc_us-gaap_StatementTable_f8f41d17-d301-4373-a3b5-6cdec834c6a6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_508e9463-5ead-4dc2-9a19-d410841bfeae" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_f8f41d17-d301-4373-a3b5-6cdec834c6a6" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_508e9463-5ead-4dc2-9a19-d410841bfeae" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_508e9463-5ead-4dc2-9a19-d410841bfeae_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_508e9463-5ead-4dc2-9a19-d410841bfeae" xlink:to="loc_us-gaap_EquityComponentDomain_508e9463-5ead-4dc2-9a19-d410841bfeae_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_30b9c4b4-fc92-40e5-8de3-9ef0c9367180" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_508e9463-5ead-4dc2-9a19-d410841bfeae" xlink:to="loc_us-gaap_EquityComponentDomain_30b9c4b4-fc92-40e5-8de3-9ef0c9367180" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_ddc21881-e41d-4b6a-9acf-b757af4764b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PreferredStockMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_30b9c4b4-fc92-40e5-8de3-9ef0c9367180" xlink:to="loc_us-gaap_PreferredStockMember_ddc21881-e41d-4b6a-9acf-b757af4764b5" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_2c410169-cbcb-4878-9dfa-9a85319d98d7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_30b9c4b4-fc92-40e5-8de3-9ef0c9367180" xlink:to="loc_us-gaap_CommonStockMember_2c410169-cbcb-4878-9dfa-9a85319d98d7" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_ef6cc4c1-a505-49f6-a566-103f800210cb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_30b9c4b4-fc92-40e5-8de3-9ef0c9367180" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_ef6cc4c1-a505-49f6-a566-103f800210cb" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_fead20dd-69c3-40e6-988a-aabc8efa2b8e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_30b9c4b4-fc92-40e5-8de3-9ef0c9367180" xlink:to="loc_us-gaap_RetainedEarningsMember_fead20dd-69c3-40e6-988a-aabc8efa2b8e" xlink:type="arc" order="3"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/ConsolidatedStatementsOfCashFlows" xlink:type="simple" xlink:href="esmc-20220630.xsd#ConsolidatedStatementsOfCashFlows"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/ConsolidatedStatementsOfCashFlows" xlink:type="extended" id="i89717a3631264b6c8b4e17b68a461128_ConsolidatedStatementsOfCashFlows">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_6adf6121-b6e9-49e3-a491-a51a312ee3bd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_6adf6121-b6e9-49e3-a491-a51a312ee3bd" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_9395a41d-7a8c-43d6-a3df-4316c1e65a14" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_6adf6121-b6e9-49e3-a491-a51a312ee3bd" xlink:to="loc_us-gaap_NetIncomeLoss_9395a41d-7a8c-43d6-a3df-4316c1e65a14" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_687d884c-9b66-4088-b999-0d5ac777b3fe" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_6adf6121-b6e9-49e3-a491-a51a312ee3bd" xlink:to="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_687d884c-9b66-4088-b999-0d5ac777b3fe" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForDoubtfulAccounts_f2985f28-8b3d-4495-8226-ee117fda1e6d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProvisionForDoubtfulAccounts"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_687d884c-9b66-4088-b999-0d5ac777b3fe" xlink:to="loc_us-gaap_ProvisionForDoubtfulAccounts_f2985f28-8b3d-4495-8226-ee117fda1e6d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncashIncomeExpense_dd5f0797-9fd3-4cef-8198-18a6f3ac9c2f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherNoncashIncomeExpense"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_687d884c-9b66-4088-b999-0d5ac777b3fe" xlink:to="loc_us-gaap_OtherNoncashIncomeExpense_dd5f0797-9fd3-4cef-8198-18a6f3ac9c2f" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_5d87333c-45e8-435b-8606-d911804bbaf0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_687d884c-9b66-4088-b999-0d5ac777b3fe" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_5d87333c-45e8-435b-8606-d911804bbaf0" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_a68ad8a3-c53c-402c-8eb1-46de3a6deb22" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherOperatingActivitiesCashFlowStatement"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_687d884c-9b66-4088-b999-0d5ac777b3fe" xlink:to="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_a68ad8a3-c53c-402c-8eb1-46de3a6deb22" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInOperatingCapitalAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_687d884c-9b66-4088-b999-0d5ac777b3fe" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_a1665f5b-c7ce-4992-b628-38f2a9d8b4a6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_a1665f5b-c7ce-4992-b628-38f2a9d8b4a6" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_30edf069-2109-4044-bbf0-d2e59e95a6b0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInInventories"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_30edf069-2109-4044-bbf0-d2e59e95a6b0" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherCurrentAssets_9801ebc2-09c7-4479-a940-a6c60f79aa57" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInOtherCurrentAssets"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherCurrentAssets_9801ebc2-09c7-4479-a940-a6c60f79aa57" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_77258d46-d08e-40a4-a4c8-5851ce9a8d2c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_77258d46-d08e-40a4-a4c8-5851ce9a8d2c" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_06144430-554f-4cbe-b31e-acea760fbf18" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_06144430-554f-4cbe-b31e-acea760fbf18" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_a38069e6-67dd-4b9b-b22f-a73035a75ed1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInOperatingLeaseLiability"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_a38069e6-67dd-4b9b-b22f-a73035a75ed1" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_edfb2e02-3844-482c-af1a-7b06982847d1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_edfb2e02-3844-482c-af1a-7b06982847d1" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherDeferredLiability_0e63b944-7e1c-4493-8ad0-4fdfb82734fb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInOtherDeferredLiability"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_f8c54ff3-4a54-4d7c-b092-04b33d31493d" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherDeferredLiability_0e63b944-7e1c-4493-8ad0-4fdfb82734fb" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_c184d34a-a486-4c92-b0c2-327f2701f288" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_6adf6121-b6e9-49e3-a491-a51a312ee3bd" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_c184d34a-a486-4c92-b0c2-327f2701f288" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a10978c2-f0c8-4ac6-a103-cb74f2479364" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a10978c2-f0c8-4ac6-a103-cb74f2479364" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireProductiveAssets_4409e06e-3e1a-4bb5-b436-eb7d648050f1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquireProductiveAssets"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a10978c2-f0c8-4ac6-a103-cb74f2479364" xlink:to="loc_us-gaap_PaymentsToAcquireProductiveAssets_4409e06e-3e1a-4bb5-b436-eb7d648050f1" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_1bbdf5a7-1529-45bd-bcf7-97363a2cf80e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a10978c2-f0c8-4ac6-a103-cb74f2479364" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_1bbdf5a7-1529-45bd-bcf7-97363a2cf80e" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfSecuredDebt_8f19d1fe-b4da-4c2b-90f5-dc84b15a66be" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RepaymentsOfSecuredDebt"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a10978c2-f0c8-4ac6-a103-cb74f2479364" xlink:to="loc_us-gaap_RepaymentsOfSecuredDebt_8f19d1fe-b4da-4c2b-90f5-dc84b15a66be" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_3642f909-1ad5-44e3-be4e-2c53386f73d6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_3642f909-1ad5-44e3-be4e-2c53386f73d6" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfNotesPayable_523c6e73-707b-47dd-9996-cffda941d956" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RepaymentsOfNotesPayable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_3642f909-1ad5-44e3-be4e-2c53386f73d6" xlink:to="loc_us-gaap_RepaymentsOfNotesPayable_523c6e73-707b-47dd-9996-cffda941d956" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_d5b2b9e1-69be-4098-aa9e-14c7ad704dc9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_d5b2b9e1-69be-4098-aa9e-14c7ad704dc9" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_88064daf-7647-4689-be48-dea281bd7615" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_88064daf-7647-4689-be48-dea281bd7615" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_afcd3162-df4a-4227-80e9-6fbac908e535" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_afcd3162-df4a-4227-80e9-6fbac908e535" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_880bd0ec-be01-4911-a47f-713bc4294417" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxesPaid_9dcecb4c-48e4-43f5-8c18-97e6d20a297e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxesPaid"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:to="loc_us-gaap_IncomeTaxesPaid_9dcecb4c-48e4-43f5-8c18-97e6d20a297e" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_58a4c27f-a0bd-48fe-9f2e-038edaaf7849" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_67c1d921-c3ea-429b-9ae4-5b8da25d3696" xlink:to="loc_us-gaap_StatementTable_58a4c27f-a0bd-48fe-9f2e-038edaaf7849" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_f65c0bd8-30bf-4338-ac42-5d92c8411100" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_58a4c27f-a0bd-48fe-9f2e-038edaaf7849" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_f65c0bd8-30bf-4338-ac42-5d92c8411100" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_f65c0bd8-30bf-4338-ac42-5d92c8411100_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_f65c0bd8-30bf-4338-ac42-5d92c8411100" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_f65c0bd8-30bf-4338-ac42-5d92c8411100_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_ccf891ef-eb8c-4b62-8368-61d64090ea3b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_f65c0bd8-30bf-4338-ac42-5d92c8411100" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_ccf891ef-eb8c-4b62-8368-61d64090ea3b" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_PayrollProtectionProgramLoanMember_74f6025f-ea3f-4a50-99da-3fbebb55867e" xlink:href="esmc-20220630.xsd#esmc_PayrollProtectionProgramLoanMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_ccf891ef-eb8c-4b62-8368-61d64090ea3b" xlink:to="loc_esmc_PayrollProtectionProgramLoanMember_74f6025f-ea3f-4a50-99da-3fbebb55867e" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_SmallBusinessAssociationLoanMember_de37ab0a-47a3-4a18-a1b8-2ad10625365a" xlink:href="esmc-20220630.xsd#esmc_SmallBusinessAssociationLoanMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_ccf891ef-eb8c-4b62-8368-61d64090ea3b" xlink:to="loc_esmc_SmallBusinessAssociationLoanMember_de37ab0a-47a3-4a18-a1b8-2ad10625365a" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/SignificantAccountingPoliciesTables" xlink:type="simple" xlink:href="esmc-20220630.xsd#SignificantAccountingPoliciesTables"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/SignificantAccountingPoliciesTables" xlink:type="extended" id="ic6f16492e0954bde891b13a22e55b2b4_SignificantAccountingPoliciesTables">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_91e293d2-8f5b-43fc-aaea-8302999b17b8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_819498cb-3aa8-4d6b-906d-a2c5db852810" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_91e293d2-8f5b-43fc-aaea-8302999b17b8" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_819498cb-3aa8-4d6b-906d-a2c5db852810" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_783a0b46-2371-46e0-a549-b3192fefa9ed" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_819498cb-3aa8-4d6b-906d-a2c5db852810" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_783a0b46-2371-46e0-a549-b3192fefa9ed" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_783a0b46-2371-46e0-a549-b3192fefa9ed_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_783a0b46-2371-46e0-a549-b3192fefa9ed" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_783a0b46-2371-46e0-a549-b3192fefa9ed_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f803195b-25a3-4515-85db-4e66dbc8196a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_783a0b46-2371-46e0-a549-b3192fefa9ed" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f803195b-25a3-4515-85db-4e66dbc8196a" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/SignificantAccountingPoliciesPropertyPlantandEquipmentTables" xlink:type="simple" xlink:href="esmc-20220630.xsd#SignificantAccountingPoliciesPropertyPlantandEquipmentTables"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/SignificantAccountingPoliciesPropertyPlantandEquipmentTables" xlink:type="extended" id="i0429400d46fc4f21872fb7c897ac0c96_SignificantAccountingPoliciesPropertyPlantandEquipmentTables">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_1ead5a2d-724a-4532-a875-4dbb71237300" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTextBlock_a5666c2e-5665-4bcc-bc3d-a0cb53587b85" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTextBlock"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_1ead5a2d-724a-4532-a875-4dbb71237300" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTextBlock_a5666c2e-5665-4bcc-bc3d-a0cb53587b85" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_4358ad2c-6169-4f68-817e-7ee950106a51" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_1ead5a2d-724a-4532-a875-4dbb71237300" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_4358ad2c-6169-4f68-817e-7ee950106a51" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_0a06ac75-09f0-414c-b244-e581a2d98f3f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_4358ad2c-6169-4f68-817e-7ee950106a51" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_0a06ac75-09f0-414c-b244-e581a2d98f3f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_0a06ac75-09f0-414c-b244-e581a2d98f3f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_0a06ac75-09f0-414c-b244-e581a2d98f3f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_0a06ac75-09f0-414c-b244-e581a2d98f3f_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_e0f544d2-b6c8-4a4d-a6c0-95e733588183" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_0a06ac75-09f0-414c-b244-e581a2d98f3f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_e0f544d2-b6c8-4a4d-a6c0-95e733588183" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_45572014-6c26-4d7b-ac3f-d78c78cc6060" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_4358ad2c-6169-4f68-817e-7ee950106a51" xlink:to="loc_srt_RangeAxis_45572014-6c26-4d7b-ac3f-d78c78cc6060" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_45572014-6c26-4d7b-ac3f-d78c78cc6060_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_45572014-6c26-4d7b-ac3f-d78c78cc6060" xlink:to="loc_srt_RangeMember_45572014-6c26-4d7b-ac3f-d78c78cc6060_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_2e3133db-e63a-40a6-91be-77146452c210" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_45572014-6c26-4d7b-ac3f-d78c78cc6060" xlink:to="loc_srt_RangeMember_2e3133db-e63a-40a6-91be-77146452c210" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/SignificantAccountingPoliciesStockBasedCompensationDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#SignificantAccountingPoliciesStockBasedCompensationDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/SignificantAccountingPoliciesStockBasedCompensationDetails" xlink:type="extended" id="i0dac752f79a04c1392ca3923458770b4_SignificantAccountingPoliciesStockBasedCompensationDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3cf67b7b-7952-49e7-b6e1-5c1d3c9e3d6e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable_3e510006-0c1d-4db3-be5b-a979208af22b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3cf67b7b-7952-49e7-b6e1-5c1d3c9e3d6e" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable_3e510006-0c1d-4db3-be5b-a979208af22b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_769d6188-0952-474f-90f8-25276daebc70" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AwardTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable_3e510006-0c1d-4db3-be5b-a979208af22b" xlink:to="loc_us-gaap_AwardTypeAxis_769d6188-0952-474f-90f8-25276daebc70" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_769d6188-0952-474f-90f8-25276daebc70_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_769d6188-0952-474f-90f8-25276daebc70" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_769d6188-0952-474f-90f8-25276daebc70_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_56a73174-7fc5-43cb-aee4-0f3e6be79b20" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_769d6188-0952-474f-90f8-25276daebc70" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_56a73174-7fc5-43cb-aee4-0f3e6be79b20" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_87c0f5f4-3e43-4e62-a503-cd923d1eb166" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable_3e510006-0c1d-4db3-be5b-a979208af22b" xlink:to="loc_srt_RangeAxis_87c0f5f4-3e43-4e62-a503-cd923d1eb166" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_87c0f5f4-3e43-4e62-a503-cd923d1eb166_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_87c0f5f4-3e43-4e62-a503-cd923d1eb166" xlink:to="loc_srt_RangeMember_87c0f5f4-3e43-4e62-a503-cd923d1eb166_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_98eaefa8-4c81-49d4-be12-7cdd6514a786" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_87c0f5f4-3e43-4e62-a503-cd923d1eb166" xlink:to="loc_srt_RangeMember_98eaefa8-4c81-49d4-be12-7cdd6514a786" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_89002b1f-62d1-470f-999c-5e0b62f5492c" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_98eaefa8-4c81-49d4-be12-7cdd6514a786" xlink:to="loc_srt_MinimumMember_89002b1f-62d1-470f-999c-5e0b62f5492c" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/SignificantAccountingPoliciesNetIncomelossPerShareDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#SignificantAccountingPoliciesNetIncomelossPerShareDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/SignificantAccountingPoliciesNetIncomelossPerShareDetails" xlink:type="extended" id="i49f21f7f33bd42da8d80e2478932f134_SignificantAccountingPoliciesNetIncomelossPerShareDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_fd71ffa5-5b23-445b-aed9-8b9ca24d21e0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_fa63fdc4-fb33-4189-9218-c1c78ce7ba6e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_fd71ffa5-5b23-445b-aed9-8b9ca24d21e0" xlink:to="loc_us-gaap_NetIncomeLoss_fa63fdc4-fb33-4189-9218-c1c78ce7ba6e" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossFromContinuingOperationsAvailableToCommonShareholdersBasic_b3b1c504-9eac-41c9-a58e-2fc78a61120c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLossFromContinuingOperationsAvailableToCommonShareholdersBasic"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_fd71ffa5-5b23-445b-aed9-8b9ca24d21e0" xlink:to="loc_us-gaap_NetIncomeLossFromContinuingOperationsAvailableToCommonShareholdersBasic_b3b1c504-9eac-41c9-a58e-2fc78a61120c" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_e71a1c30-27fc-47ef-8ad3-3dfcc1df069f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_fd71ffa5-5b23-445b-aed9-8b9ca24d21e0" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_e71a1c30-27fc-47ef-8ad3-3dfcc1df069f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_0b70b440-e5a8-4f7f-af2b-081e8fc3e666" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_e71a1c30-27fc-47ef-8ad3-3dfcc1df069f" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_0b70b440-e5a8-4f7f-af2b-081e8fc3e666" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_0b70b440-e5a8-4f7f-af2b-081e8fc3e666_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_0b70b440-e5a8-4f7f-af2b-081e8fc3e666" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_0b70b440-e5a8-4f7f-af2b-081e8fc3e666_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_09f95832-fb05-4138-8252-db371c4722e9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_0b70b440-e5a8-4f7f-af2b-081e8fc3e666" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_09f95832-fb05-4138-8252-db371c4722e9" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockOptionMember_aaa84a18-16e8-48a0-aade-d58bf107dbb9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockOptionMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_09f95832-fb05-4138-8252-db371c4722e9" xlink:to="loc_us-gaap_StockOptionMember_aaa84a18-16e8-48a0-aade-d58bf107dbb9" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertiblePreferredStockMember_089866f1-baf3-48b2-8529-a0b8ef792e0c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConvertiblePreferredStockMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_09f95832-fb05-4138-8252-db371c4722e9" xlink:to="loc_us-gaap_ConvertiblePreferredStockMember_089866f1-baf3-48b2-8529-a0b8ef792e0c" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/SignificantAccountingPoliciesEarningpersharedetailsDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#SignificantAccountingPoliciesEarningpersharedetailsDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/SignificantAccountingPoliciesEarningpersharedetailsDetails" xlink:type="extended" id="id38891886a7946b8ba3d92de2f07929f_SignificantAccountingPoliciesEarningpersharedetailsDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_437b988b-1c9a-4a45-841d-aaeefc85fcec" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_NetIncomeLoss_437b988b-1c9a-4a45-841d-aaeefc85fcec" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockDividendsIncomeStatementImpact_b30586ec-e14b-40b2-8b9e-fe6ed3902f59" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PreferredStockDividendsIncomeStatementImpact"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_PreferredStockDividendsIncomeStatementImpact_b30586ec-e14b-40b2-8b9e-fe6ed3902f59" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted_c10e3cd9-aacb-4be3-845e-21eb54db73dd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted_c10e3cd9-aacb-4be3-845e-21eb54db73dd" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_2af6f216-a2f1-443b-b24e-4b488167a50c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_2af6f216-a2f1-443b-b24e-4b488167a50c" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossFromContinuingOperationsAvailableToCommonShareholdersBasic_e01f0758-a1ed-4021-b8aa-7bfcaeab7c83" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLossFromContinuingOperationsAvailableToCommonShareholdersBasic"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_NetIncomeLossFromContinuingOperationsAvailableToCommonShareholdersBasic_e01f0758-a1ed-4021-b8aa-7bfcaeab7c83" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertiblePreferredDividendsNetOfTax_5a55ec9e-1ef9-4d0b-b6a8-ec67ab3d1451" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConvertiblePreferredDividendsNetOfTax"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_ConvertiblePreferredDividendsNetOfTax_5a55ec9e-1ef9-4d0b-b6a8-ec67ab3d1451" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DilutiveSecuritiesEffectOnBasicEarningsPerShareOther_ef0c520a-7322-449a-90a3-db057392d667" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DilutiveSecuritiesEffectOnBasicEarningsPerShareOther"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_DilutiveSecuritiesEffectOnBasicEarningsPerShareOther_ef0c520a-7322-449a-90a3-db057392d667" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_e2ed7541-4746-4045-8906-53279132bee2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_e2ed7541-4746-4045-8906-53279132bee2" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncrementalCommonSharesAttributableToConversionOfPreferredStock_c863bd74-0a51-4b9a-913d-326fdbe343dd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncrementalCommonSharesAttributableToConversionOfPreferredStock"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_IncrementalCommonSharesAttributableToConversionOfPreferredStock_c863bd74-0a51-4b9a-913d-326fdbe343dd" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_fe3d88b9-454e-46c6-ae63-bb78fa6d98fa" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_fe3d88b9-454e-46c6-ae63-bb78fa6d98fa" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_9f46b0fb-85d5-4f90-99c7-d2cdaa8d2a30" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EarningsPerShareBasic"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_EarningsPerShareBasic_9f46b0fb-85d5-4f90-99c7-d2cdaa8d2a30" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_0b9e4579-6e75-45e6-9cf7-d5bd868eae1c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EarningsPerShareDiluted"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_EarningsPerShareDiluted_0b9e4579-6e75-45e6-9cf7-d5bd868eae1c" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_d583fc26-2f96-4e91-95e7-4ce241189e9b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_f4a03322-1190-4aa4-bf3f-c291053e7908" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_d583fc26-2f96-4e91-95e7-4ce241189e9b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_bfefc6a3-1546-4831-ba3f-d1eff08398a8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_d583fc26-2f96-4e91-95e7-4ce241189e9b" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_bfefc6a3-1546-4831-ba3f-d1eff08398a8" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_bfefc6a3-1546-4831-ba3f-d1eff08398a8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_bfefc6a3-1546-4831-ba3f-d1eff08398a8" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_bfefc6a3-1546-4831-ba3f-d1eff08398a8_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_5bddba1e-b7b3-441f-b0b5-05947f0835c0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_bfefc6a3-1546-4831-ba3f-d1eff08398a8" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_5bddba1e-b7b3-441f-b0b5-05947f0835c0" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertiblePreferredStockMember_892af40b-2bc5-40d9-b7e3-4bb5aef8c01d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConvertiblePreferredStockMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_5bddba1e-b7b3-441f-b0b5-05947f0835c0" xlink:to="loc_us-gaap_ConvertiblePreferredStockMember_892af40b-2bc5-40d9-b7e3-4bb5aef8c01d" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockOptionMember_67421b41-cae9-4d9c-9c1c-7b1f57e2c75b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockOptionMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_5bddba1e-b7b3-441f-b0b5-05947f0835c0" xlink:to="loc_us-gaap_StockOptionMember_67421b41-cae9-4d9c-9c1c-7b1f57e2c75b" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/SignificantAccountingPoliciesPropertypantandequipmentDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#SignificantAccountingPoliciesPropertypantandequipmentDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/SignificantAccountingPoliciesPropertypantandequipmentDetails" xlink:type="extended" id="i44d3e4d823c940ff84f78b4f1b146b5e_SignificantAccountingPoliciesPropertypantandequipmentDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_efc458f4-ea06-4da0-8069-c55b7d646b00" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_efc458f4-ea06-4da0-8069-c55b7d646b00" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_MachineryAndEquipmentGross_94e2b279-4c74-49e3-823c-209479ac007d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MachineryAndEquipmentGross"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_MachineryAndEquipmentGross_94e2b279-4c74-49e3-823c-209479ac007d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_1af1c356-cbf1-4226-83fb-26c154845643" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_1af1c356-cbf1-4226-83fb-26c154845643" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesGross_3449ba8d-dcd4-429f-bdde-45d012122612" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FurnitureAndFixturesGross"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_FurnitureAndFixturesGross_3449ba8d-dcd4-429f-bdde-45d012122612" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsGross_601d2954-cc49-46f4-b842-e1cd12f370d8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LeaseholdImprovementsGross"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_LeaseholdImprovementsGross_601d2954-cc49-46f4-b842-e1cd12f370d8" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_f225fc2a-63a7-4dc9-b55c-c2353ef46d46" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_f225fc2a-63a7-4dc9-b55c-c2353ef46d46" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_c08c95b1-413e-4922-b108-7cb01ed2520c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_c08c95b1-413e-4922-b108-7cb01ed2520c" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_e21b767a-cee6-48a3-bc06-379e1ae59303" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_e21b767a-cee6-48a3-bc06-379e1ae59303" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a04008c7-32cc-4452-b3bf-657c9331369c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_89c97586-5003-4194-80da-352a2f2f6a14" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a04008c7-32cc-4452-b3bf-657c9331369c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis_e25eebc0-eeed-49ae-b5e6-07b4ab09b341" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a04008c7-32cc-4452-b3bf-657c9331369c" xlink:to="loc_srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis_e25eebc0-eeed-49ae-b5e6-07b4ab09b341" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_e25eebc0-eeed-49ae-b5e6-07b4ab09b341_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis_e25eebc0-eeed-49ae-b5e6-07b4ab09b341" xlink:to="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_e25eebc0-eeed-49ae-b5e6-07b4ab09b341_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_74ae2dd8-dd5c-4c6c-bb25-4aeee79d4a8f" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis_e25eebc0-eeed-49ae-b5e6-07b4ab09b341" xlink:to="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_74ae2dd8-dd5c-4c6c-bb25-4aeee79d4a8f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_d0a5f767-52c3-4045-835d-16edb0bcf287" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LeaseholdImprovementsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_74ae2dd8-dd5c-4c6c-bb25-4aeee79d4a8f" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_d0a5f767-52c3-4045-835d-16edb0bcf287" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_01d91575-2220-46e7-8ed4-b2571a42a39d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FurnitureAndFixturesMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_74ae2dd8-dd5c-4c6c-bb25-4aeee79d4a8f" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_01d91575-2220-46e7-8ed4-b2571a42a39d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentMember_00b8cd8c-9ba9-4875-9c00-9be496a4f26d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquipmentMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_74ae2dd8-dd5c-4c6c-bb25-4aeee79d4a8f" xlink:to="loc_us-gaap_EquipmentMember_00b8cd8c-9ba9-4875-9c00-9be496a4f26d" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_b5790bd2-5cab-4ef4-a78d-0a73b6c8a4cf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a04008c7-32cc-4452-b3bf-657c9331369c" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_b5790bd2-5cab-4ef4-a78d-0a73b6c8a4cf" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_b5790bd2-5cab-4ef4-a78d-0a73b6c8a4cf_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_b5790bd2-5cab-4ef4-a78d-0a73b6c8a4cf" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_b5790bd2-5cab-4ef4-a78d-0a73b6c8a4cf_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_bf047705-1e77-4ee6-a040-6145f9d224fd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_b5790bd2-5cab-4ef4-a78d-0a73b6c8a4cf" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_bf047705-1e77-4ee6-a040-6145f9d224fd" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_56beb97c-0f3e-49f2-90d0-f7e500551d92" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FurnitureAndFixturesMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_bf047705-1e77-4ee6-a040-6145f9d224fd" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_56beb97c-0f3e-49f2-90d0-f7e500551d92" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentMember_1429ad30-48f8-4ed0-88a0-ea089bfab9a7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquipmentMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_bf047705-1e77-4ee6-a040-6145f9d224fd" xlink:to="loc_us-gaap_EquipmentMember_1429ad30-48f8-4ed0-88a0-ea089bfab9a7" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_979720fe-1b98-4a98-9e06-ed14debc1b50" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a04008c7-32cc-4452-b3bf-657c9331369c" xlink:to="loc_srt_RangeAxis_979720fe-1b98-4a98-9e06-ed14debc1b50" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_979720fe-1b98-4a98-9e06-ed14debc1b50_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_979720fe-1b98-4a98-9e06-ed14debc1b50" xlink:to="loc_srt_RangeMember_979720fe-1b98-4a98-9e06-ed14debc1b50_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_5aba5b9c-bd91-4622-b4cd-d13ba57f33f3" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_979720fe-1b98-4a98-9e06-ed14debc1b50" xlink:to="loc_srt_RangeMember_5aba5b9c-bd91-4622-b4cd-d13ba57f33f3" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_bc6e7928-0c82-407b-81cd-0ce059b2df47" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5aba5b9c-bd91-4622-b4cd-d13ba57f33f3" xlink:to="loc_srt_MinimumMember_bc6e7928-0c82-407b-81cd-0ce059b2df47" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_d2d062b0-e078-4667-b548-583d1189a160" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MaximumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5aba5b9c-bd91-4622-b4cd-d13ba57f33f3" xlink:to="loc_srt_MaximumMember_d2d062b0-e078-4667-b548-583d1189a160" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/IntangibleassetsTables" xlink:type="simple" xlink:href="esmc-20220630.xsd#IntangibleassetsTables"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/IntangibleassetsTables" xlink:type="extended" id="i684ae07af45e4db587b264de501fd9b0_IntangibleassetsTables">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_643c7d3a-7d79-4e82-937d-2d7bd844f6bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTableTextBlock_a5d4b637-5edd-4979-92b4-b237cca63333" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTableTextBlock"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_643c7d3a-7d79-4e82-937d-2d7bd844f6bb" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTableTextBlock_a5d4b637-5edd-4979-92b4-b237cca63333" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfIntangibleAssetsAndGoodwillTableTextBlock_43de2baf-6758-481c-afdf-831e81372685" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfIntangibleAssetsAndGoodwillTableTextBlock"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_643c7d3a-7d79-4e82-937d-2d7bd844f6bb" xlink:to="loc_us-gaap_ScheduleOfIntangibleAssetsAndGoodwillTableTextBlock_43de2baf-6758-481c-afdf-831e81372685" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfIndefiniteLivedIntangibleAssetsTableTextBlock_48a55e32-77cc-4ad2-957c-c1544fdb4b6e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfIndefiniteLivedIntangibleAssetsTableTextBlock"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_643c7d3a-7d79-4e82-937d-2d7bd844f6bb" xlink:to="loc_us-gaap_ScheduleOfIndefiniteLivedIntangibleAssetsTableTextBlock_48a55e32-77cc-4ad2-957c-c1544fdb4b6e" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_5d83e147-4aa7-4acb-a44e-a24d86c8048c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_643c7d3a-7d79-4e82-937d-2d7bd844f6bb" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_5d83e147-4aa7-4acb-a44e-a24d86c8048c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_010ef02b-79a6-4c08-92b3-3b7224381089" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_5d83e147-4aa7-4acb-a44e-a24d86c8048c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_010ef02b-79a6-4c08-92b3-3b7224381089" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_010ef02b-79a6-4c08-92b3-3b7224381089_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_010ef02b-79a6-4c08-92b3-3b7224381089" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_010ef02b-79a6-4c08-92b3-3b7224381089_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1604ce2e-bf0e-45fe-9620-225cfe1e540f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_010ef02b-79a6-4c08-92b3-3b7224381089" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1604ce2e-bf0e-45fe-9620-225cfe1e540f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicensingAgreementsMember_7c907724-cd27-4c8f-9b83-c8ac2d639378" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LicensingAgreementsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1604ce2e-bf0e-45fe-9620-225cfe1e540f" xlink:to="loc_us-gaap_LicensingAgreementsMember_7c907724-cd27-4c8f-9b83-c8ac2d639378" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_TrademarksAndTradeNamesMember_f8576405-5c26-401b-8b17-965616a93702" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TrademarksAndTradeNamesMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1604ce2e-bf0e-45fe-9620-225cfe1e540f" xlink:to="loc_us-gaap_TrademarksAndTradeNamesMember_f8576405-5c26-401b-8b17-965616a93702" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_38a468c4-89bf-4402-9f9b-d2538c407e63" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ConsolidationItemsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_5d83e147-4aa7-4acb-a44e-a24d86c8048c" xlink:to="loc_srt_ConsolidationItemsAxis_38a468c4-89bf-4402-9f9b-d2538c407e63" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_38a468c4-89bf-4402-9f9b-d2538c407e63_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ConsolidationItemsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_38a468c4-89bf-4402-9f9b-d2538c407e63" xlink:to="loc_srt_ConsolidationItemsDomain_38a468c4-89bf-4402-9f9b-d2538c407e63_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_2019f9e1-fc7a-44bc-a1d0-d70143b8d734" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ConsolidationItemsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_38a468c4-89bf-4402-9f9b-d2538c407e63" xlink:to="loc_srt_ConsolidationItemsDomain_2019f9e1-fc7a-44bc-a1d0-d70143b8d734" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_13618ff4-0ec2-4efc-84a8-a0e3c9e9576a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_5d83e147-4aa7-4acb-a44e-a24d86c8048c" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_13618ff4-0ec2-4efc-84a8-a0e3c9e9576a" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_13618ff4-0ec2-4efc-84a8-a0e3c9e9576a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SegmentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_13618ff4-0ec2-4efc-84a8-a0e3c9e9576a" xlink:to="loc_us-gaap_SegmentDomain_13618ff4-0ec2-4efc-84a8-a0e3c9e9576a_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_25e516fe-b963-46fd-959c-a091ca7a6ba3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SegmentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_13618ff4-0ec2-4efc-84a8-a0e3c9e9576a" xlink:to="loc_us-gaap_SegmentDomain_25e516fe-b963-46fd-959c-a091ca7a6ba3" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_54d6e91a-ed61-4222-8de2-1a32f968059d" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_5d83e147-4aa7-4acb-a44e-a24d86c8048c" xlink:to="loc_srt_RangeAxis_54d6e91a-ed61-4222-8de2-1a32f968059d" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_54d6e91a-ed61-4222-8de2-1a32f968059d_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_54d6e91a-ed61-4222-8de2-1a32f968059d" xlink:to="loc_srt_RangeMember_54d6e91a-ed61-4222-8de2-1a32f968059d_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_34419c2e-06a8-4af4-9b61-bcf4ec8daf75" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_54d6e91a-ed61-4222-8de2-1a32f968059d" xlink:to="loc_srt_RangeMember_34419c2e-06a8-4af4-9b61-bcf4ec8daf75" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/IntangibleassetsDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#IntangibleassetsDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/IntangibleassetsDetails" xlink:type="extended" id="idf58a65129f8467e98b93a485ee23639_IntangibleassetsDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IndefiniteLivedIntangibleAssetsExcludingGoodwill_c2a51a3d-e3d6-440c-9b75-c3c4ef77f15e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IndefiniteLivedIntangibleAssetsExcludingGoodwill"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_IndefiniteLivedIntangibleAssetsExcludingGoodwill_c2a51a3d-e3d6-440c-9b75-c3c4ef77f15e" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_4b15f498-206c-4869-b531-546987b6ebf3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_4b15f498-206c-4869-b531-546987b6ebf3" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_ca33f3ec-0b23-4d19-9d3f-dd38c5270b61" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_ca33f3ec-0b23-4d19-9d3f-dd38c5270b61" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireOtherProductiveAssets_ca5b57b3-25eb-4994-8d3f-7b139e1f6813" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquireOtherProductiveAssets"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_PaymentsToAcquireOtherProductiveAssets_ca5b57b3-25eb-4994-8d3f-7b139e1f6813" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_557a3307-7d36-44f8-86b3-4b5e2a0f4632" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_557a3307-7d36-44f8-86b3-4b5e2a0f4632" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearFive_ecf25d2a-e499-4091-a60e-0256a323c734" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearFive"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearFive_ecf25d2a-e499-4091-a60e-0256a323c734" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseAfterYearFive_3135777a-d079-4755-9f3a-b9cf229c578e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseAfterYearFive"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseAfterYearFive_3135777a-d079-4755-9f3a-b9cf229c578e" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_3e73b471-077b-4ae3-bf29-c5850ee75991" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_3e73b471-077b-4ae3-bf29-c5850ee75991" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_Finitelivedtangibleassetsaccumulatedimpairment_e738eeca-f590-4c59-b54a-1b0608c5236f" xlink:href="esmc-20220630.xsd#esmc_Finitelivedtangibleassetsaccumulatedimpairment"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_esmc_Finitelivedtangibleassetsaccumulatedimpairment_e738eeca-f590-4c59-b54a-1b0608c5236f" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_Finitelivedintangibleassetsnetofimpairment_48333fd9-49f0-441d-983c-9fe9f9d75faa" xlink:href="esmc-20220630.xsd#esmc_Finitelivedintangibleassetsnetofimpairment"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_esmc_Finitelivedintangibleassetsnetofimpairment_48333fd9-49f0-441d-983c-9fe9f9d75faa" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationMethod_e9851de8-f5a7-42ec-826f-19b56a4af8e1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationMethod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationMethod_e9851de8-f5a7-42ec-826f-19b56a4af8e1" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearTwo_364f3499-eb54-491b-9d85-9c2ab6b0c956" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearTwo"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearTwo_364f3499-eb54-491b-9d85-9c2ab6b0c956" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRollingAfterYearFive_231462fe-4d40-49d6-a2ee-2e9fdbb709f8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRollingAfterYearFive"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRollingAfterYearFive_231462fe-4d40-49d6-a2ee-2e9fdbb709f8" xlink:type="arc" order="12"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRollingYearTwo_27fdbcd9-b0aa-482a-8daa-cd749cc71741" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRollingYearTwo"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRollingYearTwo_27fdbcd9-b0aa-482a-8daa-cd749cc71741" xlink:type="arc" order="13"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_7e225754-4efd-4c69-92d8-0ce43bd50510" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_dfa11476-a8c6-46da-99fe-d7495af27bc7" xlink:to="loc_us-gaap_StatementTable_7e225754-4efd-4c69-92d8-0ce43bd50510" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_63ddc5d4-f2b2-4166-b5dc-dadb831a1077" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7e225754-4efd-4c69-92d8-0ce43bd50510" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_63ddc5d4-f2b2-4166-b5dc-dadb831a1077" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_63ddc5d4-f2b2-4166-b5dc-dadb831a1077_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_63ddc5d4-f2b2-4166-b5dc-dadb831a1077" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_63ddc5d4-f2b2-4166-b5dc-dadb831a1077_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_859e74ce-794c-4ce8-9b78-b9dc57c73a04" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_63ddc5d4-f2b2-4166-b5dc-dadb831a1077" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_859e74ce-794c-4ce8-9b78-b9dc57c73a04" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicensingAgreementsMember_27cfb2b9-65fc-4923-821e-b54d69688a75" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LicensingAgreementsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_859e74ce-794c-4ce8-9b78-b9dc57c73a04" xlink:to="loc_us-gaap_LicensingAgreementsMember_27cfb2b9-65fc-4923-821e-b54d69688a75" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_ca954f3d-eeee-4753-9a0d-b4374dc702da" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7e225754-4efd-4c69-92d8-0ce43bd50510" xlink:to="loc_srt_RangeAxis_ca954f3d-eeee-4753-9a0d-b4374dc702da" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ca954f3d-eeee-4753-9a0d-b4374dc702da_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_ca954f3d-eeee-4753-9a0d-b4374dc702da" xlink:to="loc_srt_RangeMember_ca954f3d-eeee-4753-9a0d-b4374dc702da_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d18cf1de-a40c-4a76-bfeb-77a2c53819cc" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_ca954f3d-eeee-4753-9a0d-b4374dc702da" xlink:to="loc_srt_RangeMember_d18cf1de-a40c-4a76-bfeb-77a2c53819cc" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_06cf2c81-6413-4b36-8820-348f8a58dae9" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MaximumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d18cf1de-a40c-4a76-bfeb-77a2c53819cc" xlink:to="loc_srt_MaximumMember_06cf2c81-6413-4b36-8820-348f8a58dae9" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_31591907-0357-4513-b2c0-8e786837f6ec" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_StatementScenarioAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7e225754-4efd-4c69-92d8-0ce43bd50510" xlink:to="loc_srt_StatementScenarioAxis_31591907-0357-4513-b2c0-8e786837f6ec" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_31591907-0357-4513-b2c0-8e786837f6ec_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ScenarioUnspecifiedDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_31591907-0357-4513-b2c0-8e786837f6ec" xlink:to="loc_srt_ScenarioUnspecifiedDomain_31591907-0357-4513-b2c0-8e786837f6ec_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_93609699-cd60-4c0a-a66b-308e320e75ba" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ScenarioUnspecifiedDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_31591907-0357-4513-b2c0-8e786837f6ec" xlink:to="loc_srt_ScenarioUnspecifiedDomain_93609699-cd60-4c0a-a66b-308e320e75ba" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioForecastMember_cc6583d3-785c-44ef-be62-96d607fa262b" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ScenarioForecastMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_93609699-cd60-4c0a-a66b-308e320e75ba" xlink:to="loc_srt_ScenarioForecastMember_cc6583d3-785c-44ef-be62-96d607fa262b" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/LineofcreditDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#LineofcreditDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/LineofcreditDetails" xlink:type="extended" id="id61264ded3284c0f9c9b498cdb4814c4_LineofcreditDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityFairValueOfAmountOutstanding_18cca372-f5e7-468a-9bfd-7b5f92cfc747" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityFairValueOfAmountOutstanding"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_LineOfCreditFacilityFairValueOfAmountOutstanding_18cca372-f5e7-468a-9bfd-7b5f92cfc747" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityPeriodicPaymentInterest_3f5237b6-5e9d-41b7-8b7f-e13335c581f0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityPeriodicPaymentInterest"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_LineOfCreditFacilityPeriodicPaymentInterest_3f5237b6-5e9d-41b7-8b7f-e13335c581f0" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableToBank_d1fa6a77-dfb2-44b6-aac8-b5e277d35b23" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NotesPayableToBank"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_NotesPayableToBank_d1fa6a77-dfb2-44b6-aac8-b5e277d35b23" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_52a20db1-2f1b-4ce9-9fa3-8de7ffcf8a5c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_52a20db1-2f1b-4ce9-9fa3-8de7ffcf8a5c" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_594321e2-b239-4cfe-a2f9-7a5a387345f3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_594321e2-b239-4cfe-a2f9-7a5a387345f3" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateDuringPeriod_a2a205dc-afb0-44e5-82eb-454d79a3487e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentInterestRateDuringPeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_DebtInstrumentInterestRateDuringPeriod_a2a205dc-afb0-44e5-82eb-454d79a3487e" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_0ac81083-5e04-424b-9c99-9584dbd42e76" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_0ac81083-5e04-424b-9c99-9584dbd42e76" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCollateralFeesAmount_dbecc31a-d59c-45a7-bef6-9b1859074a89" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityCollateralFeesAmount"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_LineOfCreditFacilityCollateralFeesAmount_dbecc31a-d59c-45a7-bef6-9b1859074a89" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_6a717ffd-4df1-47c3-b3fd-675365001fdc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_77c4009c-4591-4741-9ae3-29d4e384d195" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_6a717ffd-4df1-47c3-b3fd-675365001fdc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_dda8447a-c8ec-4ea0-bfa8-b98c6defb5e7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_6a717ffd-4df1-47c3-b3fd-675365001fdc" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_dda8447a-c8ec-4ea0-bfa8-b98c6defb5e7" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_dda8447a-c8ec-4ea0-bfa8-b98c6defb5e7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_dda8447a-c8ec-4ea0-bfa8-b98c6defb5e7" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_dda8447a-c8ec-4ea0-bfa8-b98c6defb5e7_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_f4bc19e0-decf-4ffe-9c1f-af3ddddebe8a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_dda8447a-c8ec-4ea0-bfa8-b98c6defb5e7" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_f4bc19e0-decf-4ffe-9c1f-af3ddddebe8a" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableToBanksMember_30cf6c32-a57d-4b6c-acc8-cd64ff35ebd4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NotesPayableToBanksMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_f4bc19e0-decf-4ffe-9c1f-af3ddddebe8a" xlink:to="loc_us-gaap_NotesPayableToBanksMember_30cf6c32-a57d-4b6c-acc8-cd64ff35ebd4" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_1294acaa-b0fb-4b6e-acc9-64397fbd6fcc" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_6a717ffd-4df1-47c3-b3fd-675365001fdc" xlink:to="loc_srt_RangeAxis_1294acaa-b0fb-4b6e-acc9-64397fbd6fcc" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_1294acaa-b0fb-4b6e-acc9-64397fbd6fcc_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_1294acaa-b0fb-4b6e-acc9-64397fbd6fcc" xlink:to="loc_srt_RangeMember_1294acaa-b0fb-4b6e-acc9-64397fbd6fcc_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_276d53a2-7d84-42cc-91e4-c802a6860dd3" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_1294acaa-b0fb-4b6e-acc9-64397fbd6fcc" xlink:to="loc_srt_RangeMember_276d53a2-7d84-42cc-91e4-c802a6860dd3" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_c3bc0671-d575-433c-9ef6-24282e17f6df" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_276d53a2-7d84-42cc-91e4-c802a6860dd3" xlink:to="loc_srt_MinimumMember_c3bc0671-d575-433c-9ef6-24282e17f6df" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_af833b65-16aa-48b5-bcce-3b614d39f77a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_6a717ffd-4df1-47c3-b3fd-675365001fdc" xlink:to="loc_us-gaap_VariableRateAxis_af833b65-16aa-48b5-bcce-3b614d39f77a" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_af833b65-16aa-48b5-bcce-3b614d39f77a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_af833b65-16aa-48b5-bcce-3b614d39f77a" xlink:to="loc_us-gaap_VariableRateDomain_af833b65-16aa-48b5-bcce-3b614d39f77a_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_1229ec31-a591-4443-b432-9f3e4da70711" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_af833b65-16aa-48b5-bcce-3b614d39f77a" xlink:to="loc_us-gaap_VariableRateDomain_1229ec31-a591-4443-b432-9f3e4da70711" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrimeRateMember_109dac3d-0fcf-4d48-892b-6707397ba2d1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PrimeRateMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_1229ec31-a591-4443-b432-9f3e4da70711" xlink:to="loc_us-gaap_PrimeRateMember_109dac3d-0fcf-4d48-892b-6707397ba2d1" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityAxis_f83ce0e8-af56-4313-95ef-9512f14f232c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_6a717ffd-4df1-47c3-b3fd-675365001fdc" xlink:to="loc_us-gaap_LineOfCreditFacilityAxis_f83ce0e8-af56-4313-95ef-9512f14f232c" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_f83ce0e8-af56-4313-95ef-9512f14f232c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_f83ce0e8-af56-4313-95ef-9512f14f232c" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_f83ce0e8-af56-4313-95ef-9512f14f232c_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_5e726650-1dfc-4f0e-a2c1-2eef733824cf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_f83ce0e8-af56-4313-95ef-9512f14f232c" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_5e726650-1dfc-4f0e-a2c1-2eef733824cf" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_34a0594d-7b6a-43dd-9967-d0cca2852ac0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_6a717ffd-4df1-47c3-b3fd-675365001fdc" xlink:to="loc_us-gaap_CreditFacilityAxis_34a0594d-7b6a-43dd-9967-d0cca2852ac0" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_34a0594d-7b6a-43dd-9967-d0cca2852ac0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_34a0594d-7b6a-43dd-9967-d0cca2852ac0" xlink:to="loc_us-gaap_CreditFacilityDomain_34a0594d-7b6a-43dd-9967-d0cca2852ac0_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_a92f13a4-e969-4e25-93d3-57b9a5bd08b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_34a0594d-7b6a-43dd-9967-d0cca2852ac0" xlink:to="loc_us-gaap_CreditFacilityDomain_a92f13a4-e969-4e25-93d3-57b9a5bd08b3" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_488afb01-9d5a-4298-b179-6c1f91d0ae9f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_a92f13a4-e969-4e25-93d3-57b9a5bd08b3" xlink:to="loc_us-gaap_LineOfCreditMember_488afb01-9d5a-4298-b179-6c1f91d0ae9f" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/LongtermDebtTables" xlink:type="simple" xlink:href="esmc-20220630.xsd#LongtermDebtTables"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/LongtermDebtTables" xlink:type="extended" id="ia86e53a013df411a9860bde34eb55f82_LongtermDebtTables">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_1f37a8b6-63e2-478d-b269-2b1f28b2ffe1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_e1392ea9-5490-4039-8c74-ff7d80b5debf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_1f37a8b6-63e2-478d-b269-2b1f28b2ffe1" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_e1392ea9-5490-4039-8c74-ff7d80b5debf" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityAxis_c89190ba-8216-4e66-a76f-73a6edf80805" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_e1392ea9-5490-4039-8c74-ff7d80b5debf" xlink:to="loc_us-gaap_LineOfCreditFacilityAxis_c89190ba-8216-4e66-a76f-73a6edf80805" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_c89190ba-8216-4e66-a76f-73a6edf80805_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_c89190ba-8216-4e66-a76f-73a6edf80805" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_c89190ba-8216-4e66-a76f-73a6edf80805_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_a62479a8-c560-45d5-a594-b11aa30fd890" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_c89190ba-8216-4e66-a76f-73a6edf80805" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_a62479a8-c560-45d5-a594-b11aa30fd890" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_917ce46b-0cb3-43a7-b19c-c89a62c4e95f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_e1392ea9-5490-4039-8c74-ff7d80b5debf" xlink:to="loc_us-gaap_CreditFacilityAxis_917ce46b-0cb3-43a7-b19c-c89a62c4e95f" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_917ce46b-0cb3-43a7-b19c-c89a62c4e95f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_917ce46b-0cb3-43a7-b19c-c89a62c4e95f" xlink:to="loc_us-gaap_CreditFacilityDomain_917ce46b-0cb3-43a7-b19c-c89a62c4e95f_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_a0c6d0f9-e910-43df-a629-9fe6517bf5df" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_917ce46b-0cb3-43a7-b19c-c89a62c4e95f" xlink:to="loc_us-gaap_CreditFacilityDomain_a0c6d0f9-e910-43df-a629-9fe6517bf5df" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/LongtermDebtEIDLmaturitiesTables" xlink:type="simple" xlink:href="esmc-20220630.xsd#LongtermDebtEIDLmaturitiesTables"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/LongtermDebtEIDLmaturitiesTables" xlink:type="extended" id="iaf44429817b54cc0a79f447cc82619a0_LongtermDebtEIDLmaturitiesTables">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_22f56b77-ee1b-48ef-b004-50f95c241881" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfMaturitiesOfLongTermDebtTableTextBlock_4cf942cf-5354-454d-bb97-500fad11f610" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfMaturitiesOfLongTermDebtTableTextBlock"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_22f56b77-ee1b-48ef-b004-50f95c241881" xlink:to="loc_us-gaap_ScheduleOfMaturitiesOfLongTermDebtTableTextBlock_4cf942cf-5354-454d-bb97-500fad11f610" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_744f6207-cced-4f7f-9d01-386124fbcaa4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_22f56b77-ee1b-48ef-b004-50f95c241881" xlink:to="loc_us-gaap_DebtInstrumentTable_744f6207-cced-4f7f-9d01-386124fbcaa4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_4b0aaa64-c386-4d8f-bd88-114c052daabc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_744f6207-cced-4f7f-9d01-386124fbcaa4" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_4b0aaa64-c386-4d8f-bd88-114c052daabc" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_4b0aaa64-c386-4d8f-bd88-114c052daabc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_4b0aaa64-c386-4d8f-bd88-114c052daabc" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_4b0aaa64-c386-4d8f-bd88-114c052daabc_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_e67f76d7-3d63-48ce-9568-7b28df8c0a3e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_4b0aaa64-c386-4d8f-bd88-114c052daabc" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_e67f76d7-3d63-48ce-9568-7b28df8c0a3e" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_f3d093e8-5a43-4380-ba77-41b3dbe0ddb0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_744f6207-cced-4f7f-9d01-386124fbcaa4" xlink:to="loc_us-gaap_DebtInstrumentAxis_f3d093e8-5a43-4380-ba77-41b3dbe0ddb0" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_f3d093e8-5a43-4380-ba77-41b3dbe0ddb0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_f3d093e8-5a43-4380-ba77-41b3dbe0ddb0" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_f3d093e8-5a43-4380-ba77-41b3dbe0ddb0_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_e0874b7c-6451-4c44-bbc3-355210d805e0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_f3d093e8-5a43-4380-ba77-41b3dbe0ddb0" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_e0874b7c-6451-4c44-bbc3-355210d805e0" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_f1f2a084-06d1-4c42-af24-473bece4dc5e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_744f6207-cced-4f7f-9d01-386124fbcaa4" xlink:to="loc_us-gaap_VariableRateAxis_f1f2a084-06d1-4c42-af24-473bece4dc5e" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_f1f2a084-06d1-4c42-af24-473bece4dc5e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_f1f2a084-06d1-4c42-af24-473bece4dc5e" xlink:to="loc_us-gaap_VariableRateDomain_f1f2a084-06d1-4c42-af24-473bece4dc5e_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_4bc1f421-f8a5-40f6-84d5-b66f0c6290f5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_f1f2a084-06d1-4c42-af24-473bece4dc5e" xlink:to="loc_us-gaap_VariableRateDomain_4bc1f421-f8a5-40f6-84d5-b66f0c6290f5" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_bd99b90d-397c-4e92-a1d4-783347ccd8f3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentRedemptionPeriodAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_744f6207-cced-4f7f-9d01-386124fbcaa4" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_bd99b90d-397c-4e92-a1d4-783347ccd8f3" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_bd99b90d-397c-4e92-a1d4-783347ccd8f3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentRedemptionPeriodDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_bd99b90d-397c-4e92-a1d4-783347ccd8f3" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_bd99b90d-397c-4e92-a1d4-783347ccd8f3_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_f6c290b9-3e68-43c8-ade0-9d367d53ce17" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentRedemptionPeriodDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_bd99b90d-397c-4e92-a1d4-783347ccd8f3" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_f6c290b9-3e68-43c8-ade0-9d367d53ce17" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_9e6825d9-c006-4c52-ba60-f63b024ee7ff" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_744f6207-cced-4f7f-9d01-386124fbcaa4" xlink:to="loc_srt_RangeAxis_9e6825d9-c006-4c52-ba60-f63b024ee7ff" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_9e6825d9-c006-4c52-ba60-f63b024ee7ff_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_9e6825d9-c006-4c52-ba60-f63b024ee7ff" xlink:to="loc_srt_RangeMember_9e6825d9-c006-4c52-ba60-f63b024ee7ff_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_b788175b-48b3-4662-bab6-7a93e2f2fd8c" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_9e6825d9-c006-4c52-ba60-f63b024ee7ff" xlink:to="loc_srt_RangeMember_b788175b-48b3-4662-bab6-7a93e2f2fd8c" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/LongtermDebtDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#LongtermDebtDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/LongtermDebtDetails" xlink:type="extended" id="ia56e76b981204a4794161b50ad7e85e9_LongtermDebtDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLongTermDebt_865455c6-0137-4591-be45-c84c80602014" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherLongTermDebt"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_OtherLongTermDebt_865455c6-0137-4591-be45-c84c80602014" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_455186a8-04c5-4efb-841f-f64bee299421" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_455186a8-04c5-4efb-841f-f64bee299421" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_PPPloanamount_13f0d9b8-52db-4857-ae86-83131181d485" xlink:href="esmc-20220630.xsd#esmc_PPPloanamount"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_esmc_PPPloanamount_13f0d9b8-52db-4857-ae86-83131181d485" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentTerms_5445dc6c-5b1c-4a69-8c10-25e420fcaf8a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentTerms"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentTerms_5445dc6c-5b1c-4a69-8c10-25e420fcaf8a" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_b963abed-e4d9-4b67-9f3d-c719f862e5d1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_b963abed-e4d9-4b67-9f3d-c719f862e5d1" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_237a9f82-e029-4795-919c-f87ca418dc7e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_237a9f82-e029-4795-919c-f87ca418dc7e" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour_3e26783c-b41e-4660-a21b-b6b0cf0693de" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour_3e26783c-b41e-4660-a21b-b6b0cf0693de" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive_e64aa5e3-706a-4517-895e-89f164d515e8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive_e64aa5e3-706a-4517-895e-89f164d515e8" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalAfterYearFive_c0be96c3-1694-4e24-bf4e-3a4e497817c5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalAfterYearFive"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalAfterYearFive_c0be96c3-1694-4e24-bf4e-3a4e497817c5" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_219c34b0-c8f5-46e2-adc2-c9069d4df25e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebt"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebt_219c34b0-c8f5-46e2-adc2-c9069d4df25e" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtPercentageBearingFixedInterestRate_1df03343-3fc3-44af-bd0a-59492305d30a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtPercentageBearingFixedInterestRate"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_LongTermDebtPercentageBearingFixedInterestRate_1df03343-3fc3-44af-bd0a-59492305d30a" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_EIDLMonthlyPaymentincludingPrincipalandInterest_edb8719d-566a-481f-acb0-2ba4906eb424" xlink:href="esmc-20220630.xsd#esmc_EIDLMonthlyPaymentincludingPrincipalandInterest"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_esmc_EIDLMonthlyPaymentincludingPrincipalandInterest_edb8719d-566a-481f-acb0-2ba4906eb424" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_6c31d7c1-d6d1-4662-b3f5-666a27c32753" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b455370c-1c1e-40e5-be6d-54f7e15d0a87" xlink:to="loc_us-gaap_DebtInstrumentTable_6c31d7c1-d6d1-4662-b3f5-666a27c32753" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_f9e9b028-3431-44f1-84fd-460d6e03f555" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6c31d7c1-d6d1-4662-b3f5-666a27c32753" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_f9e9b028-3431-44f1-84fd-460d6e03f555" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_f9e9b028-3431-44f1-84fd-460d6e03f555_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_f9e9b028-3431-44f1-84fd-460d6e03f555" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_f9e9b028-3431-44f1-84fd-460d6e03f555_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_135f63bd-dd74-4d53-89a1-8aec8e56be4b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_f9e9b028-3431-44f1-84fd-460d6e03f555" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_135f63bd-dd74-4d53-89a1-8aec8e56be4b" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_91a83b15-6fea-41d6-b9c7-67c241d1adda" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SecuredDebtMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_135f63bd-dd74-4d53-89a1-8aec8e56be4b" xlink:to="loc_us-gaap_SecuredDebtMember_91a83b15-6fea-41d6-b9c7-67c241d1adda" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnsecuredDebtMember_81851265-7944-491d-942c-717c205110fd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_UnsecuredDebtMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_135f63bd-dd74-4d53-89a1-8aec8e56be4b" xlink:to="loc_us-gaap_UnsecuredDebtMember_81851265-7944-491d-942c-717c205110fd" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_2b181a66-b4f0-476f-959d-6dd7892334b7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6c31d7c1-d6d1-4662-b3f5-666a27c32753" xlink:to="loc_us-gaap_DebtInstrumentAxis_2b181a66-b4f0-476f-959d-6dd7892334b7" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_2b181a66-b4f0-476f-959d-6dd7892334b7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_2b181a66-b4f0-476f-959d-6dd7892334b7" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_2b181a66-b4f0-476f-959d-6dd7892334b7_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_c0570e4d-633d-420f-a627-f5076e047f15" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_2b181a66-b4f0-476f-959d-6dd7892334b7" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_c0570e4d-633d-420f-a627-f5076e047f15" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_792181ed-21f4-4c60-b273-c2ffc177e5c7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6c31d7c1-d6d1-4662-b3f5-666a27c32753" xlink:to="loc_us-gaap_VariableRateAxis_792181ed-21f4-4c60-b273-c2ffc177e5c7" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_792181ed-21f4-4c60-b273-c2ffc177e5c7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_792181ed-21f4-4c60-b273-c2ffc177e5c7" xlink:to="loc_us-gaap_VariableRateDomain_792181ed-21f4-4c60-b273-c2ffc177e5c7_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_139fee37-02dc-4c76-8ea8-4f4846771569" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_VariableRateDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_792181ed-21f4-4c60-b273-c2ffc177e5c7" xlink:to="loc_us-gaap_VariableRateDomain_139fee37-02dc-4c76-8ea8-4f4846771569" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_d19b7726-b457-45cc-9a66-1d5dc0daf431" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentRedemptionPeriodAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6c31d7c1-d6d1-4662-b3f5-666a27c32753" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_d19b7726-b457-45cc-9a66-1d5dc0daf431" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_d19b7726-b457-45cc-9a66-1d5dc0daf431_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentRedemptionPeriodDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_d19b7726-b457-45cc-9a66-1d5dc0daf431" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_d19b7726-b457-45cc-9a66-1d5dc0daf431_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_c950488c-3096-4d4d-89e1-38d1b0ec1eb6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentRedemptionPeriodDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_d19b7726-b457-45cc-9a66-1d5dc0daf431" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_c950488c-3096-4d4d-89e1-38d1b0ec1eb6" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_d76a771e-fc89-452a-b406-3495d7201742" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6c31d7c1-d6d1-4662-b3f5-666a27c32753" xlink:to="loc_srt_RangeAxis_d76a771e-fc89-452a-b406-3495d7201742" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d76a771e-fc89-452a-b406-3495d7201742_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_d76a771e-fc89-452a-b406-3495d7201742" xlink:to="loc_srt_RangeMember_d76a771e-fc89-452a-b406-3495d7201742_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_b589d076-36ae-4f49-ad2d-bda181b3fbed" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_d76a771e-fc89-452a-b406-3495d7201742" xlink:to="loc_srt_RangeMember_b589d076-36ae-4f49-ad2d-bda181b3fbed" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/CapitalStockTransactionsStockOptionPlansNarrativeDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#CapitalStockTransactionsStockOptionPlansNarrativeDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/CapitalStockTransactionsStockOptionPlansNarrativeDetails" xlink:type="extended" id="ib103fd29f0ba4a6bb2455863735f1465_CapitalStockTransactionsStockOptionPlansNarrativeDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_8949839b-781f-45e2-a525-003edf9ba317" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_8949839b-781f-45e2-a525-003edf9ba317" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions_bfc95b52-84b9-4d76-9875-72515afa7be6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions_bfc95b52-84b9-4d76-9875-72515afa7be6" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_f58fd4cb-cac4-4c91-8223-e385b6d1c562" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_f58fd4cb-cac4-4c91-8223-e385b6d1c562" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_2324067b-fd5c-42d0-8314-9656f2110419" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_2324067b-fd5c-42d0-8314-9656f2110419" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfPlans_47b21313-5854-4785-931c-e1a51c34144a" xlink:href="esmc-20220630.xsd#esmc_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfPlans"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_esmc_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfPlans_47b21313-5854-4785-931c-e1a51c34144a" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_db5246a3-d924-492a-bb98-a63fefde0b03" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_db5246a3-d924-492a-bb98-a63fefde0b03" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_a7680016-8793-4ac8-aed7-e6dd2de7876e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_a7680016-8793-4ac8-aed7-e6dd2de7876e" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisePeriod_01cbb70d-116b-4b41-bb7b-c4c4b17ef5e7" xlink:href="esmc-20220630.xsd#esmc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisePeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_esmc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisePeriod_01cbb70d-116b-4b41-bb7b-c4c4b17ef5e7" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_6be55cbf-e2ee-478b-a868-3cddf37bcf4f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_6be55cbf-e2ee-478b-a868-3cddf37bcf4f" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_2aa85239-a8ef-4eba-a0b7-92f402cf62cc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_2aa85239-a8ef-4eba-a0b7-92f402cf62cc" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ShareBasedCompensationArrangementbyShareBasedPaymentAwardUnvested_ace16297-5983-493b-bf3f-bd8ef7fa59f0" xlink:href="esmc-20220630.xsd#esmc_ShareBasedCompensationArrangementbyShareBasedPaymentAwardUnvested"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_esmc_ShareBasedCompensationArrangementbyShareBasedPaymentAwardUnvested_ace16297-5983-493b-bf3f-bd8ef7fa59f0" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f8341b77-ec90-4b9e-adba-d108e3cd25b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_92825bb9-15bd-499f-82a1-e81835e2f2b7" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f8341b77-ec90-4b9e-adba-d108e3cd25b5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_d65eb5d2-b245-4017-94a6-940357cc4de0" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f8341b77-ec90-4b9e-adba-d108e3cd25b5" xlink:to="loc_srt_RangeAxis_d65eb5d2-b245-4017-94a6-940357cc4de0" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d65eb5d2-b245-4017-94a6-940357cc4de0_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_d65eb5d2-b245-4017-94a6-940357cc4de0" xlink:to="loc_srt_RangeMember_d65eb5d2-b245-4017-94a6-940357cc4de0_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ba10e67d-57b8-4a5d-9d8f-c1806dec58ef" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_d65eb5d2-b245-4017-94a6-940357cc4de0" xlink:to="loc_srt_RangeMember_ba10e67d-57b8-4a5d-9d8f-c1806dec58ef" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_941420d5-0446-4ac7-9a4b-4e9c1c1f23f9" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ba10e67d-57b8-4a5d-9d8f-c1806dec58ef" xlink:to="loc_srt_MinimumMember_941420d5-0446-4ac7-9a4b-4e9c1c1f23f9" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_f9b82059-bec2-40e7-8cfc-40c1dce079af" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MaximumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ba10e67d-57b8-4a5d-9d8f-c1806dec58ef" xlink:to="loc_srt_MaximumMember_f9b82059-bec2-40e7-8cfc-40c1dce079af" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_709656f5-0724-40f1-96f9-dbc367ed86c9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f8341b77-ec90-4b9e-adba-d108e3cd25b5" xlink:to="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_709656f5-0724-40f1-96f9-dbc367ed86c9" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_709656f5-0724-40f1-96f9-dbc367ed86c9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_709656f5-0724-40f1-96f9-dbc367ed86c9" xlink:to="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_709656f5-0724-40f1-96f9-dbc367ed86c9_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_f77021ab-9aa5-453e-8042-7cf67c782e99" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_709656f5-0724-40f1-96f9-dbc367ed86c9" xlink:to="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_f77021ab-9aa5-453e-8042-7cf67c782e99" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ExercisePriceRangeThreeMember_ea4ee229-e426-4e5e-b1ef-6420edca914b" xlink:href="esmc-20220630.xsd#esmc_ExercisePriceRangeThreeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_f77021ab-9aa5-453e-8042-7cf67c782e99" xlink:to="loc_esmc_ExercisePriceRangeThreeMember_ea4ee229-e426-4e5e-b1ef-6420edca914b" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ExercisePriceRangeOneMember_9584ebf2-ea31-4184-ae3d-f47e7fe4d47a" xlink:href="esmc-20220630.xsd#esmc_ExercisePriceRangeOneMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_f77021ab-9aa5-453e-8042-7cf67c782e99" xlink:to="loc_esmc_ExercisePriceRangeOneMember_9584ebf2-ea31-4184-ae3d-f47e7fe4d47a" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_7825287f-8a1d-4de6-a235-5081b0058d8c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f8341b77-ec90-4b9e-adba-d108e3cd25b5" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_7825287f-8a1d-4de6-a235-5081b0058d8c" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_7825287f-8a1d-4de6-a235-5081b0058d8c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_7825287f-8a1d-4de6-a235-5081b0058d8c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_7825287f-8a1d-4de6-a235-5081b0058d8c_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_8df76014-f2a0-4b55-a285-14f34a8cb4be" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_7825287f-8a1d-4de6-a235-5081b0058d8c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_8df76014-f2a0-4b55-a285-14f34a8cb4be" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_b43985f2-5dfa-435d-bdc0-5b94608dd949" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AwardTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f8341b77-ec90-4b9e-adba-d108e3cd25b5" xlink:to="loc_us-gaap_AwardTypeAxis_b43985f2-5dfa-435d-bdc0-5b94608dd949" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b43985f2-5dfa-435d-bdc0-5b94608dd949_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_b43985f2-5dfa-435d-bdc0-5b94608dd949" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b43985f2-5dfa-435d-bdc0-5b94608dd949_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e5a672cb-b15f-4da3-b28d-79d1e914da06" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_b43985f2-5dfa-435d-bdc0-5b94608dd949" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e5a672cb-b15f-4da3-b28d-79d1e914da06" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_68d3ba06-4099-43d7-a952-ee4c0ac21442" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeStockOptionMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e5a672cb-b15f-4da3-b28d-79d1e914da06" xlink:to="loc_us-gaap_EmployeeStockOptionMember_68d3ba06-4099-43d7-a952-ee4c0ac21442" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/CapitalStockTransactionsStockOptionActivityDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#CapitalStockTransactionsStockOptionActivityDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/CapitalStockTransactionsStockOptionActivityDetails" xlink:type="extended" id="ia374885d0903467eac9a36230887da1d_CapitalStockTransactionsStockOptionActivityDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_ec13f51a-1f15-4411-8bbd-1c817679acf4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_ec13f51a-1f15-4411-8bbd-1c817679acf4" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_c8e41a9b-8863-404e-8c98-87467b4ea756" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_c8e41a9b-8863-404e-8c98-87467b4ea756" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_0c6c5102-02a5-46c8-a4ec-93edf8698268" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_0c6c5102-02a5-46c8-a4ec-93edf8698268" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_d1799c77-e848-44ec-a8bb-924abfe00a09" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_d1799c77-e848-44ec-a8bb-924abfe00a09" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions_d8d752e5-6754-4b50-9b03-26a82034b50a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions_d8d752e5-6754-4b50-9b03-26a82034b50a" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_3e203306-9bfd-4dad-806b-011ded2641dd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_3e203306-9bfd-4dad-806b-011ded2641dd" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_53eef293-2c45-4fd3-aa45-015d6c853cb6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_53eef293-2c45-4fd3-aa45-015d6c853cb6" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_f50f2012-ec4a-4b73-bee3-e14e32ef4a3f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_f50f2012-ec4a-4b73-bee3-e14e32ef4a3f" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions_8a49fb97-3d40-4d8e-a48d-9a5678e60e45" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions_8a49fb97-3d40-4d8e-a48d-9a5678e60e45" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_083429ab-64b6-42b0-a50c-9a7d5181a7b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_083429ab-64b6-42b0-a50c-9a7d5181a7b3" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_b9ee880c-846e-4952-afbf-67a8796b72f7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_b9ee880c-846e-4952-afbf-67a8796b72f7" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_f55bb2a6-09cf-45b9-9447-e86f5239ebf5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_f55bb2a6-09cf-45b9-9447-e86f5239ebf5" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_e539deca-fe4c-40bb-b6a6-8ec040c04907" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_e539deca-fe4c-40bb-b6a6-8ec040c04907" xlink:type="arc" order="12"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4306ad96-d45d-485c-9576-c0a33731fe4a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_c057cd30-84f8-4591-ac8c-be7b0762939b" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4306ad96-d45d-485c-9576-c0a33731fe4a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_48f959ec-3379-4ff8-b35d-8acd682e35bd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4306ad96-d45d-485c-9576-c0a33731fe4a" xlink:to="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_48f959ec-3379-4ff8-b35d-8acd682e35bd" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_48f959ec-3379-4ff8-b35d-8acd682e35bd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_48f959ec-3379-4ff8-b35d-8acd682e35bd" xlink:to="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_48f959ec-3379-4ff8-b35d-8acd682e35bd_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_02e9c4db-5e75-4844-9eb3-cc3a85ba3b71" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_48f959ec-3379-4ff8-b35d-8acd682e35bd" xlink:to="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_02e9c4db-5e75-4844-9eb3-cc3a85ba3b71" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ExercisePriceRangeThreeMember_b8b45066-3368-4ce5-9e7a-fb9726ff5e08" xlink:href="esmc-20220630.xsd#esmc_ExercisePriceRangeThreeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_02e9c4db-5e75-4844-9eb3-cc3a85ba3b71" xlink:to="loc_esmc_ExercisePriceRangeThreeMember_b8b45066-3368-4ce5-9e7a-fb9726ff5e08" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ExercisePriceRangeOneMember_d00230b6-8fac-4623-a437-62f12ddc02e6" xlink:href="esmc-20220630.xsd#esmc_ExercisePriceRangeOneMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_02e9c4db-5e75-4844-9eb3-cc3a85ba3b71" xlink:to="loc_esmc_ExercisePriceRangeOneMember_d00230b6-8fac-4623-a437-62f12ddc02e6" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_d963357e-4622-4893-8c35-bbbbf11cadde" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4306ad96-d45d-485c-9576-c0a33731fe4a" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_d963357e-4622-4893-8c35-bbbbf11cadde" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_d963357e-4622-4893-8c35-bbbbf11cadde_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_d963357e-4622-4893-8c35-bbbbf11cadde" xlink:to="loc_us-gaap_EquityComponentDomain_d963357e-4622-4893-8c35-bbbbf11cadde_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_75929a4a-1211-4571-992e-019b5f65a732" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_d963357e-4622-4893-8c35-bbbbf11cadde" xlink:to="loc_us-gaap_EquityComponentDomain_75929a4a-1211-4571-992e-019b5f65a732" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/CapitalStockTransactionsCompensationrelatedcostDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#CapitalStockTransactionsCompensationrelatedcostDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/CapitalStockTransactionsCompensationrelatedcostDetails" xlink:type="extended" id="i927961f1bb554516b66d2c52acefe733_CapitalStockTransactionsCompensationrelatedcostDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionLineItems_d538e8de-0319-4475-a67e-ea71454e8983" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedGoodsAndNonemployeeServicesTransactionTable_87fcafc3-2213-4fb6-930a-b969f6085436" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedGoodsAndNonemployeeServicesTransactionTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionLineItems_d538e8de-0319-4475-a67e-ea71454e8983" xlink:to="loc_us-gaap_ScheduleOfShareBasedGoodsAndNonemployeeServicesTransactionTable_87fcafc3-2213-4fb6-930a-b969f6085436" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_47513fe7-04ad-41f4-9c9c-ab336aa80dd6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AwardTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedGoodsAndNonemployeeServicesTransactionTable_87fcafc3-2213-4fb6-930a-b969f6085436" xlink:to="loc_us-gaap_AwardTypeAxis_47513fe7-04ad-41f4-9c9c-ab336aa80dd6" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_47513fe7-04ad-41f4-9c9c-ab336aa80dd6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_47513fe7-04ad-41f4-9c9c-ab336aa80dd6" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_47513fe7-04ad-41f4-9c9c-ab336aa80dd6_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_7adb194b-766c-4d4e-a8c6-97a052dd3c3c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_47513fe7-04ad-41f4-9c9c-ab336aa80dd6" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_7adb194b-766c-4d4e-a8c6-97a052dd3c3c" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis_688313d2-ab8e-4c4c-aad3-50893c7f7927" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedGoodsAndNonemployeeServicesTransactionTable_87fcafc3-2213-4fb6-930a-b969f6085436" xlink:to="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis_688313d2-ab8e-4c4c-aad3-50893c7f7927" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_688313d2-ab8e-4c4c-aad3-50893c7f7927_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis_688313d2-ab8e-4c4c-aad3-50893c7f7927" xlink:to="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_688313d2-ab8e-4c4c-aad3-50893c7f7927_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_73ffebec-3b47-417f-8642-8c79c87ebc2a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis_688313d2-ab8e-4c4c-aad3-50893c7f7927" xlink:to="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_73ffebec-3b47-417f-8642-8c79c87ebc2a" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/IncomeTaxesOperationlosscarryforwardDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#IncomeTaxesOperationlosscarryforwardDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/IncomeTaxesOperationlosscarryforwardDetails" xlink:type="extended" id="ie8bc09a77ef14cc48cfa11c22a793668_IncomeTaxesOperationlosscarryforwardDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsLineItems_30450018-6287-4d31-b66a-6f3fe93f2aa9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLossCarryforwardsLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardValuationAllowance_706ff4fd-70f1-4ebd-b171-2ecc02e61f3b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TaxCreditCarryforwardValuationAllowance"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_30450018-6287-4d31-b66a-6f3fe93f2aa9" xlink:to="loc_us-gaap_TaxCreditCarryforwardValuationAllowance_706ff4fd-70f1-4ebd-b171-2ecc02e61f3b" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsValuationAllowance_6cd9f242-6f4c-4fbf-8f09-2aebcf974207" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsValuationAllowance"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_30450018-6287-4d31-b66a-6f3fe93f2aa9" xlink:to="loc_us-gaap_DeferredTaxAssetsValuationAllowance_6cd9f242-6f4c-4fbf-8f09-2aebcf974207" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount_c3399bac-9cff-45c6-babc-63d1ba63daa1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_30450018-6287-4d31-b66a-6f3fe93f2aa9" xlink:to="loc_us-gaap_ValuationAllowanceDeferredTaxAssetChangeInAmount_c3399bac-9cff-45c6-babc-63d1ba63daa1" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwards_285a3229-23c2-4d7a-9d34-115fe5070479" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLossCarryforwards"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_30450018-6287-4d31-b66a-6f3fe93f2aa9" xlink:to="loc_us-gaap_OperatingLossCarryforwards_285a3229-23c2-4d7a-9d34-115fe5070479" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_NetOperatingLossExpirationPeriod_a1720a5b-064f-41af-b084-d2bad2d2cf27" xlink:href="esmc-20220630.xsd#esmc_NetOperatingLossExpirationPeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_30450018-6287-4d31-b66a-6f3fe93f2aa9" xlink:to="loc_esmc_NetOperatingLossExpirationPeriod_a1720a5b-064f-41af-b084-d2bad2d2cf27" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsOperatingLossCarryforwardsNotSubjectToExpiration_382874c1-91ec-4f95-90c5-49cb5b999fc7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsOperatingLossCarryforwardsNotSubjectToExpiration"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_30450018-6287-4d31-b66a-6f3fe93f2aa9" xlink:to="loc_us-gaap_DeferredTaxAssetsOperatingLossCarryforwardsNotSubjectToExpiration_382874c1-91ec-4f95-90c5-49cb5b999fc7" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsTable_08767b9e-814b-40e1-8032-416f355c29fa" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLossCarryforwardsTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_30450018-6287-4d31-b66a-6f3fe93f2aa9" xlink:to="loc_us-gaap_OperatingLossCarryforwardsTable_08767b9e-814b-40e1-8032-416f355c29fa" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_491018be-3474-4db9-a11e-e77918d33d7b" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_08767b9e-814b-40e1-8032-416f355c29fa" xlink:to="loc_srt_RangeAxis_491018be-3474-4db9-a11e-e77918d33d7b" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_491018be-3474-4db9-a11e-e77918d33d7b_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_491018be-3474-4db9-a11e-e77918d33d7b" xlink:to="loc_srt_RangeMember_491018be-3474-4db9-a11e-e77918d33d7b_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_787927ee-d612-4883-a421-fc40a2d4ab7f" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_491018be-3474-4db9-a11e-e77918d33d7b" xlink:to="loc_srt_RangeMember_787927ee-d612-4883-a421-fc40a2d4ab7f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_470f9bb7-e4c5-47eb-8722-ce682a06c0be" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_787927ee-d612-4883-a421-fc40a2d4ab7f" xlink:to="loc_srt_MinimumMember_470f9bb7-e4c5-47eb-8722-ce682a06c0be" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_5b5739d2-9c74-4b2d-bbfc-89d0872ce868" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MaximumMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_787927ee-d612-4883-a421-fc40a2d4ab7f" xlink:to="loc_srt_MaximumMember_5b5739d2-9c74-4b2d-bbfc-89d0872ce868" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_OperatingLossCarryforwardExpirationPeriodAxis_485139bf-853d-43fc-9b36-74edd689adf0" xlink:href="esmc-20220630.xsd#esmc_OperatingLossCarryforwardExpirationPeriodAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_08767b9e-814b-40e1-8032-416f355c29fa" xlink:to="loc_esmc_OperatingLossCarryforwardExpirationPeriodAxis_485139bf-853d-43fc-9b36-74edd689adf0" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_Within24YearsDomain_485139bf-853d-43fc-9b36-74edd689adf0_default" xlink:href="esmc-20220630.xsd#esmc_Within24YearsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_esmc_OperatingLossCarryforwardExpirationPeriodAxis_485139bf-853d-43fc-9b36-74edd689adf0" xlink:to="loc_esmc_Within24YearsDomain_485139bf-853d-43fc-9b36-74edd689adf0_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_Within24YearsDomain_826d933d-f88d-4d73-a368-3daf7cd0d01d" xlink:href="esmc-20220630.xsd#esmc_Within24YearsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_esmc_OperatingLossCarryforwardExpirationPeriodAxis_485139bf-853d-43fc-9b36-74edd689adf0" xlink:to="loc_esmc_Within24YearsDomain_826d933d-f88d-4d73-a368-3daf7cd0d01d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ExpirationPeriodOneMember_01157190-dca7-49c9-9230-b39e37ec5c86" xlink:href="esmc-20220630.xsd#esmc_ExpirationPeriodOneMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_esmc_Within24YearsDomain_826d933d-f88d-4d73-a368-3daf7cd0d01d" xlink:to="loc_esmc_ExpirationPeriodOneMember_01157190-dca7-49c9-9230-b39e37ec5c86" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ExpirationPeriodTwoMember_6e6eee87-a0ce-4743-912b-318402c546a7" xlink:href="esmc-20220630.xsd#esmc_ExpirationPeriodTwoMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_esmc_Within24YearsDomain_826d933d-f88d-4d73-a368-3daf7cd0d01d" xlink:to="loc_esmc_ExpirationPeriodTwoMember_6e6eee87-a0ce-4743-912b-318402c546a7" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_OperatingLossCarryforwardExpirationPeriodDomain_d954af93-e8d9-4d1a-97a2-c9893d3c7f07" xlink:href="esmc-20220630.xsd#esmc_OperatingLossCarryforwardExpirationPeriodDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_esmc_OperatingLossCarryforwardExpirationPeriodAxis_485139bf-853d-43fc-9b36-74edd689adf0" xlink:to="loc_esmc_OperatingLossCarryforwardExpirationPeriodDomain_d954af93-e8d9-4d1a-97a2-c9893d3c7f07" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_359f8ec8-7607-4295-a392-aa64a0401848" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_08767b9e-814b-40e1-8032-416f355c29fa" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_359f8ec8-7607-4295-a392-aa64a0401848" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_359f8ec8-7607-4295-a392-aa64a0401848_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_359f8ec8-7607-4295-a392-aa64a0401848" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_359f8ec8-7607-4295-a392-aa64a0401848_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_da22982e-25aa-4f45-971c-929bf66638b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_359f8ec8-7607-4295-a392-aa64a0401848" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_da22982e-25aa-4f45-971c-929bf66638b5" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalRevenueServiceIRSMember_fb60b1ee-1735-4183-b850-8a3b6cf0e35f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InternalRevenueServiceIRSMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_da22982e-25aa-4f45-971c-929bf66638b5" xlink:to="loc_us-gaap_InternalRevenueServiceIRSMember_fb60b1ee-1735-4183-b850-8a3b6cf0e35f" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_517b2ebd-bb7f-4d56-96df-c021aa913ec7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_da22982e-25aa-4f45-971c-929bf66638b5" xlink:to="loc_us-gaap_StateAndLocalJurisdictionMember_517b2ebd-bb7f-4d56-96df-c021aa913ec7" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RestatementAxis_5e3b67ea-30d5-43bb-9875-a1ef5d1c2658" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RestatementAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_08767b9e-814b-40e1-8032-416f355c29fa" xlink:to="loc_srt_RestatementAxis_5e3b67ea-30d5-43bb-9875-a1ef5d1c2658" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RestatementDomain_5e3b67ea-30d5-43bb-9875-a1ef5d1c2658_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RestatementDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RestatementAxis_5e3b67ea-30d5-43bb-9875-a1ef5d1c2658" xlink:to="loc_srt_RestatementDomain_5e3b67ea-30d5-43bb-9875-a1ef5d1c2658_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RestatementDomain_83b20a40-7454-45dd-8931-42e5ae234e4c" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RestatementDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RestatementAxis_5e3b67ea-30d5-43bb-9875-a1ef5d1c2658" xlink:to="loc_srt_RestatementDomain_83b20a40-7454-45dd-8931-42e5ae234e4c" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/RelatedPartyTransactionsDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#RelatedPartyTransactionsDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/RelatedPartyTransactionsDetails" xlink:type="extended" id="i0fd528996d9f4797af0d2d6f1f8a39c2_RelatedPartyTransactionsDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDueFromToRelatedParty_422db946-b8c6-4a9a-b2b4-f70ebe464c01" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RelatedPartyTransactionDueFromToRelatedParty"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:to="loc_us-gaap_RelatedPartyTransactionDueFromToRelatedParty_422db946-b8c6-4a9a-b2b4-f70ebe464c01" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockVotingRights_4fccbcb6-a0b5-4305-a577-dcb288dc4a93" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockVotingRights"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:to="loc_us-gaap_CommonStockVotingRights_4fccbcb6-a0b5-4305-a577-dcb288dc4a93" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertiblePreferredStockSharesIssuedUponConversion_24801db1-5b82-4b81-8687-9a20241f107a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConvertiblePreferredStockSharesIssuedUponConversion"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:to="loc_us-gaap_ConvertiblePreferredStockSharesIssuedUponConversion_24801db1-5b82-4b81-8687-9a20241f107a" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockConversionBasis_9b09c210-5d78-449f-ad54-252db4713193" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockConversionBasis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:to="loc_us-gaap_CommonStockConversionBasis_9b09c210-5d78-449f-ad54-252db4713193" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_ConvertiblePreferredStockPercentageOfOutstandingSharesOfCommonStockUponConversion_bd2dd55f-2723-467d-a875-81a284afeea4" xlink:href="esmc-20220630.xsd#esmc_ConvertiblePreferredStockPercentageOfOutstandingSharesOfCommonStockUponConversion"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:to="loc_esmc_ConvertiblePreferredStockPercentageOfOutstandingSharesOfCommonStockUponConversion_bd2dd55f-2723-467d-a875-81a284afeea4" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsPayableAmountPerShare_5d7fc8b0-94bc-4258-80b7-b3506f795891" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DividendsPayableAmountPerShare"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:to="loc_us-gaap_DividendsPayableAmountPerShare_5d7fc8b0-94bc-4258-80b7-b3506f795891" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockAmountOfPreferredDividendsInArrears_0d7d7107-c545-46b4-ae23-45c7a05dc9c3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PreferredStockAmountOfPreferredDividendsInArrears"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:to="loc_us-gaap_PreferredStockAmountOfPreferredDividendsInArrears_0d7d7107-c545-46b4-ae23-45c7a05dc9c3" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_95ef3618-bc80-4d72-9962-c0822b35dd6e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_51995184-0956-470d-b880-f432eca7c32e" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_95ef3618-bc80-4d72-9962-c0822b35dd6e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis_f1ad988c-9552-4905-9889-a95359bfa2b4" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_95ef3618-bc80-4d72-9962-c0822b35dd6e" xlink:to="loc_srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis_f1ad988c-9552-4905-9889-a95359bfa2b4" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_EquityMethodInvesteeNameDomain_f1ad988c-9552-4905-9889-a95359bfa2b4_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_EquityMethodInvesteeNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis_f1ad988c-9552-4905-9889-a95359bfa2b4" xlink:to="loc_srt_EquityMethodInvesteeNameDomain_f1ad988c-9552-4905-9889-a95359bfa2b4_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_EquityMethodInvesteeNameDomain_fae96d5f-e07a-472d-9ca6-09594ace078c" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_EquityMethodInvesteeNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis_f1ad988c-9552-4905-9889-a95359bfa2b4" xlink:to="loc_srt_EquityMethodInvesteeNameDomain_fae96d5f-e07a-472d-9ca6-09594ace078c" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeAxis_69990917-7e62-43ae-a05c-849904aa5ac9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_95ef3618-bc80-4d72-9962-c0822b35dd6e" xlink:to="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeAxis_69990917-7e62-43ae-a05c-849904aa5ac9" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeDomain_69990917-7e62-43ae-a05c-849904aa5ac9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeAxis_69990917-7e62-43ae-a05c-849904aa5ac9" xlink:to="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeDomain_69990917-7e62-43ae-a05c-849904aa5ac9_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeDomain_4be3b6c7-dd63-46e2-9649-a473e42547fa" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeAxis_69990917-7e62-43ae-a05c-849904aa5ac9" xlink:to="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeDomain_4be3b6c7-dd63-46e2-9649-a473e42547fa" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeOrGroupOfInvesteesMember_5dfbe35a-e85c-480c-a996-c0e45eb3d26a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeOrGroupOfInvesteesMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeDomain_4be3b6c7-dd63-46e2-9649-a473e42547fa" xlink:to="loc_us-gaap_EquityMethodInvestmentNonconsolidatedInvesteeOrGroupOfInvesteesMember_5dfbe35a-e85c-480c-a996-c0e45eb3d26a" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_70726efd-abf0-4024-8b4f-dc42a8f52e3b" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_OwnershipAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_95ef3618-bc80-4d72-9962-c0822b35dd6e" xlink:to="loc_srt_OwnershipAxis_70726efd-abf0-4024-8b4f-dc42a8f52e3b" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_70726efd-abf0-4024-8b4f-dc42a8f52e3b_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_OwnershipDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_70726efd-abf0-4024-8b4f-dc42a8f52e3b" xlink:to="loc_srt_OwnershipDomain_70726efd-abf0-4024-8b4f-dc42a8f52e3b_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_5c24cbcf-65e4-4c86-8b29-aeb87b06ffaa" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_OwnershipDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_70726efd-abf0-4024-8b4f-dc42a8f52e3b" xlink:to="loc_srt_OwnershipDomain_5c24cbcf-65e4-4c86-8b29-aeb87b06ffaa" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/ConcentrationofcreditriskNotes" xlink:type="simple" xlink:href="esmc-20220630.xsd#ConcentrationofcreditriskNotes"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/ConcentrationofcreditriskNotes" xlink:type="extended" id="i4064aa7222714dedb2fa80a36ad215ec_ConcentrationofcreditriskNotes">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_fe52d9c9-7735-45e2-b633-7af5cd8f9003" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskDisclosureTextBlock_5158cb29-d600-4a2f-9021-e5d480eb0d66" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskDisclosureTextBlock"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_fe52d9c9-7735-45e2-b633-7af5cd8f9003" xlink:to="loc_us-gaap_ConcentrationRiskDisclosureTextBlock_5158cb29-d600-4a2f-9021-e5d480eb0d66" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_e02291b9-9cfa-494d-a9b4-51bf7449f7cc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_fe52d9c9-7735-45e2-b633-7af5cd8f9003" xlink:to="loc_us-gaap_ConcentrationRiskTable_e02291b9-9cfa-494d-a9b4-51bf7449f7cc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_892eab31-d75c-4b99-b8fd-6e91b69af95d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_e02291b9-9cfa-494d-a9b4-51bf7449f7cc" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_892eab31-d75c-4b99-b8fd-6e91b69af95d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_892eab31-d75c-4b99-b8fd-6e91b69af95d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_892eab31-d75c-4b99-b8fd-6e91b69af95d" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_892eab31-d75c-4b99-b8fd-6e91b69af95d_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_5c4f6f20-04cd-48fd-9ee2-a384c1aaa454" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_892eab31-d75c-4b99-b8fd-6e91b69af95d" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_5c4f6f20-04cd-48fd-9ee2-a384c1aaa454" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_e8e617e1-ced2-4405-9e5b-c70cb5ac0a2b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_e02291b9-9cfa-494d-a9b4-51bf7449f7cc" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_e8e617e1-ced2-4405-9e5b-c70cb5ac0a2b" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_e8e617e1-ced2-4405-9e5b-c70cb5ac0a2b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_e8e617e1-ced2-4405-9e5b-c70cb5ac0a2b" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_e8e617e1-ced2-4405-9e5b-c70cb5ac0a2b_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_bf8bd78f-71d6-4f9c-bf8c-2997842695da" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_e8e617e1-ced2-4405-9e5b-c70cb5ac0a2b" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_bf8bd78f-71d6-4f9c-bf8c-2997842695da" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/ConcentrationofcreditriskTables" xlink:type="simple" xlink:href="esmc-20220630.xsd#ConcentrationofcreditriskTables"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/ConcentrationofcreditriskTables" xlink:type="extended" id="i0a49feea774e4b14ad111202bbd36d16_ConcentrationofcreditriskTables">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_421ec96d-f44e-4448-964f-be5dd898d8d7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SchedulesOfConcentrationOfRiskByRiskFactorTextBlock_86a852aa-6be5-40a4-909c-075b97b77619" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SchedulesOfConcentrationOfRiskByRiskFactorTextBlock"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_421ec96d-f44e-4448-964f-be5dd898d8d7" xlink:to="loc_us-gaap_SchedulesOfConcentrationOfRiskByRiskFactorTextBlock_86a852aa-6be5-40a4-909c-075b97b77619" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_47a3115f-0a6c-47af-8b0d-447befa97c75" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_421ec96d-f44e-4448-964f-be5dd898d8d7" xlink:to="loc_us-gaap_ConcentrationRiskTable_47a3115f-0a6c-47af-8b0d-447befa97c75" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_05fee2d0-cb4d-40b7-ab87-7c18be4c2b06" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_47a3115f-0a6c-47af-8b0d-447befa97c75" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_05fee2d0-cb4d-40b7-ab87-7c18be4c2b06" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_05fee2d0-cb4d-40b7-ab87-7c18be4c2b06_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_05fee2d0-cb4d-40b7-ab87-7c18be4c2b06" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_05fee2d0-cb4d-40b7-ab87-7c18be4c2b06_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_e13ccd2b-be3c-4823-94a6-b8c80f778918" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_05fee2d0-cb4d-40b7-ab87-7c18be4c2b06" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_e13ccd2b-be3c-4823-94a6-b8c80f778918" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_4657faa2-660e-436a-9fa1-90a2a52234b7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_47a3115f-0a6c-47af-8b0d-447befa97c75" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_4657faa2-660e-436a-9fa1-90a2a52234b7" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_4657faa2-660e-436a-9fa1-90a2a52234b7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_4657faa2-660e-436a-9fa1-90a2a52234b7" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_4657faa2-660e-436a-9fa1-90a2a52234b7_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_61908f59-b9ad-4bf0-a67f-e70d52866720" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_4657faa2-660e-436a-9fa1-90a2a52234b7" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_61908f59-b9ad-4bf0-a67f-e70d52866720" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.escalonmed.com/role/ConcentrationofcreditriskDetails" xlink:type="simple" xlink:href="esmc-20220630.xsd#ConcentrationofcreditriskDetails"/>
<link:definitionLink xlink:role="http://www.escalonmed.com/role/ConcentrationofcreditriskDetails" xlink:type="extended" id="ie6672ea434744218a56fe6c528638efb_ConcentrationofcreditriskDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_7a9007a4-066a-47d7-9f19-b3bd49a354f2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskCustomer_0eba25c9-5d10-4f50-8b92-d96b13a61666" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskCustomer"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_7a9007a4-066a-47d7-9f19-b3bd49a354f2" xlink:to="loc_us-gaap_ConcentrationRiskCustomer_0eba25c9-5d10-4f50-8b92-d96b13a61666" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskSupplier_fe2d30a6-c108-4d48-bca0-dd32d2fe2266" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskSupplier"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_7a9007a4-066a-47d7-9f19-b3bd49a354f2" xlink:to="loc_us-gaap_ConcentrationRiskSupplier_fe2d30a6-c108-4d48-bca0-dd32d2fe2266" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_6771daae-1627-4fbf-863c-64cbc617830a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Revenues"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_7a9007a4-066a-47d7-9f19-b3bd49a354f2" xlink:to="loc_us-gaap_Revenues_6771daae-1627-4fbf-863c-64cbc617830a" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_79ee0968-938f-4a16-84b5-07e190d3f04d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskPercentage1"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_7a9007a4-066a-47d7-9f19-b3bd49a354f2" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_79ee0968-938f-4a16-84b5-07e190d3f04d" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_af98baee-e1dd-48c8-b027-30662364ff5a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_7a9007a4-066a-47d7-9f19-b3bd49a354f2" xlink:to="loc_us-gaap_NetIncomeLoss_af98baee-e1dd-48c8-b027-30662364ff5a" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_c41f1959-b80a-40c3-b6ac-1c65005be46c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_7a9007a4-066a-47d7-9f19-b3bd49a354f2" xlink:to="loc_us-gaap_ConcentrationRiskTable_c41f1959-b80a-40c3-b6ac-1c65005be46c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis_9935465c-26f7-4542-b0ac-8ab5a5ad3ec0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_c41f1959-b80a-40c3-b6ac-1c65005be46c" xlink:to="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis_9935465c-26f7-4542-b0ac-8ab5a5ad3ec0" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_9935465c-26f7-4542-b0ac-8ab5a5ad3ec0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis_9935465c-26f7-4542-b0ac-8ab5a5ad3ec0" xlink:to="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_9935465c-26f7-4542-b0ac-8ab5a5ad3ec0_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_686fc4dc-ecff-44fb-ab02-71bdd4df7c4c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionBySupplierAxis_9935465c-26f7-4542-b0ac-8ab5a5ad3ec0" xlink:to="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_686fc4dc-ecff-44fb-ab02-71bdd4df7c4c" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_SupplieroneMember_635fbf77-b1ea-4328-ae78-e6a2abd3ffd7" xlink:href="esmc-20220630.xsd#esmc_SupplieroneMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_686fc4dc-ecff-44fb-ab02-71bdd4df7c4c" xlink:to="loc_esmc_SupplieroneMember_635fbf77-b1ea-4328-ae78-e6a2abd3ffd7" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_SuppliertwoMemberMember_dfcc0b35-cd59-474d-8b3a-5ad35860ef77" xlink:href="esmc-20220630.xsd#esmc_SuppliertwoMemberMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_686fc4dc-ecff-44fb-ab02-71bdd4df7c4c" xlink:to="loc_esmc_SuppliertwoMemberMember_dfcc0b35-cd59-474d-8b3a-5ad35860ef77" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_SupplierThreeMember_d310581a-9367-4622-89d1-d91fb21a22a6" xlink:href="esmc-20220630.xsd#esmc_SupplierThreeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedGoodsAndNonemployeeServicesTransactionSupplierDomain_686fc4dc-ecff-44fb-ab02-71bdd4df7c4c" xlink:to="loc_esmc_SupplierThreeMember_d310581a-9367-4622-89d1-d91fb21a22a6" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_eaa2c86d-2583-46fc-9527-d78b165d143c" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_StatementGeographicalAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_c41f1959-b80a-40c3-b6ac-1c65005be46c" xlink:to="loc_srt_StatementGeographicalAxis_eaa2c86d-2583-46fc-9527-d78b165d143c" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_eaa2c86d-2583-46fc-9527-d78b165d143c_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_SegmentGeographicalDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_eaa2c86d-2583-46fc-9527-d78b165d143c" xlink:to="loc_srt_SegmentGeographicalDomain_eaa2c86d-2583-46fc-9527-d78b165d143c_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_e56c579f-5973-4dae-9244-6d0f62f82609" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_SegmentGeographicalDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_eaa2c86d-2583-46fc-9527-d78b165d143c" xlink:to="loc_srt_SegmentGeographicalDomain_e56c579f-5973-4dae-9244-6d0f62f82609" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_country_US_ff8bc8eb-a102-496a-b444-f0f829f17366" xlink:href="https://xbrl.sec.gov/country/2022/country-2022.xsd#country_US"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_e56c579f-5973-4dae-9244-6d0f62f82609" xlink:to="loc_country_US_ff8bc8eb-a102-496a-b444-f0f829f17366" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_dc0a40da-2793-4cc0-9151-d477a7d2143e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NonUsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_e56c579f-5973-4dae-9244-6d0f62f82609" xlink:to="loc_us-gaap_NonUsMember_dc0a40da-2793-4cc0-9151-d477a7d2143e" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_cf21cb9c-7e09-4040-8759-71066bfa19d8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_c41f1959-b80a-40c3-b6ac-1c65005be46c" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_cf21cb9c-7e09-4040-8759-71066bfa19d8" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_cf21cb9c-7e09-4040-8759-71066bfa19d8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_cf21cb9c-7e09-4040-8759-71066bfa19d8" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_cf21cb9c-7e09-4040-8759-71066bfa19d8_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_b23e58b0-d427-48d0-9700-51486d0148d4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_cf21cb9c-7e09-4040-8759-71066bfa19d8" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_b23e58b0-d427-48d0-9700-51486d0148d4" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableMember_b8224a1a-6800-4cd5-ae8b-c1bb6007474e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsReceivableMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_b23e58b0-d427-48d0-9700-51486d0148d4" xlink:to="loc_us-gaap_AccountsReceivableMember_b8224a1a-6800-4cd5-ae8b-c1bb6007474e" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_AccountsPayableBenchmarkMember_dafa9047-f871-4bd6-aee0-d82f5c1320d9" xlink:href="esmc-20220630.xsd#esmc_AccountsPayableBenchmarkMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_b23e58b0-d427-48d0-9700-51486d0148d4" xlink:to="loc_esmc_AccountsPayableBenchmarkMember_dafa9047-f871-4bd6-aee0-d82f5c1320d9" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesRevenueNetMember_41711298-62ed-4760-ba4f-9837612c1fad" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SalesRevenueNetMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_b23e58b0-d427-48d0-9700-51486d0148d4" xlink:to="loc_us-gaap_SalesRevenueNetMember_41711298-62ed-4760-ba4f-9837612c1fad" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_13034b5a-c1ef-43af-be8a-3b5d9271ba9c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_c41f1959-b80a-40c3-b6ac-1c65005be46c" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_13034b5a-c1ef-43af-be8a-3b5d9271ba9c" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_13034b5a-c1ef-43af-be8a-3b5d9271ba9c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_13034b5a-c1ef-43af-be8a-3b5d9271ba9c" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_13034b5a-c1ef-43af-be8a-3b5d9271ba9c_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_a0ce81c3-ccee-4823-b5c2-ec01d86fb222" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_13034b5a-c1ef-43af-be8a-3b5d9271ba9c" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_a0ce81c3-ccee-4823-b5c2-ec01d86fb222" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SupplierConcentrationRiskMember_3a51833d-0156-431a-ac89-db9de23b33c2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SupplierConcentrationRiskMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_a0ce81c3-ccee-4823-b5c2-ec01d86fb222" xlink:to="loc_us-gaap_SupplierConcentrationRiskMember_3a51833d-0156-431a-ac89-db9de23b33c2" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_d9ebb02d-aa8c-4d9d-a807-3e5fb3a48602" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CustomerConcentrationRiskMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_a0ce81c3-ccee-4823-b5c2-ec01d86fb222" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_d9ebb02d-aa8c-4d9d-a807-3e5fb3a48602" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeographicConcentrationRiskMember_6c979ec4-2d11-4cfb-b020-b13d1949b31f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GeographicConcentrationRiskMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_a0ce81c3-ccee-4823-b5c2-ec01d86fb222" xlink:to="loc_us-gaap_GeographicConcentrationRiskMember_6c979ec4-2d11-4cfb-b020-b13d1949b31f" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_41e4f647-fc07-4889-bc01-57e326e38017" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MajorCustomersAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_c41f1959-b80a-40c3-b6ac-1c65005be46c" xlink:to="loc_srt_MajorCustomersAxis_41e4f647-fc07-4889-bc01-57e326e38017" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_41e4f647-fc07-4889-bc01-57e326e38017_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_NameOfMajorCustomerDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_41e4f647-fc07-4889-bc01-57e326e38017" xlink:to="loc_srt_NameOfMajorCustomerDomain_41e4f647-fc07-4889-bc01-57e326e38017_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_a3fdd187-7cdb-4501-b75f-7d4b02cf6be2" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_NameOfMajorCustomerDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_41e4f647-fc07-4889-bc01-57e326e38017" xlink:to="loc_srt_NameOfMajorCustomerDomain_a3fdd187-7cdb-4501-b75f-7d4b02cf6be2" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_esmc_CustomerOneMember_df0fceb9-20f4-4a7f-9a5a-cce615ea6c7d" xlink:href="esmc-20220630.xsd#esmc_CustomerOneMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_a3fdd187-7cdb-4501-b75f-7d4b02cf6be2" xlink:to="loc_esmc_CustomerOneMember_df0fceb9-20f4-4a7f-9a5a-cce615ea6c7d" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_e8560c85-1854-4a3f-9e03-a391f966a227" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_c41f1959-b80a-40c3-b6ac-1c65005be46c" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_e8560c85-1854-4a3f-9e03-a391f966a227" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_e8560c85-1854-4a3f-9e03-a391f966a227_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e8560c85-1854-4a3f-9e03-a391f966a227" xlink:to="loc_us-gaap_EquityComponentDomain_e8560c85-1854-4a3f-9e03-a391f966a227_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_848b8de6-b25c-4d12-832b-083f87c11a0e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e8560c85-1854-4a3f-9e03-a391f966a227" xlink:to="loc_us-gaap_EquityComponentDomain_848b8de6-b25c-4d12-832b-083f87c11a0e" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_0a4c7d22-b15d-4e3d-82b0-59317e74044f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_848b8de6-b25c-4d12-832b-083f87c11a0e" xlink:to="loc_us-gaap_RetainedEarningsMember_0a4c7d22-b15d-4e3d-82b0-59317e74044f" xlink:type="arc" order="0"/>
</link:definitionLink>
</link:linkbase>

Top
Filing Submission 0000862668-22-000023   –   Alternative Formats (Word / Rich Text, HTML, Plain Text, et al.)

Copyright © 2024 Fran Finnegan & Company LLC – All Rights Reserved.
AboutPrivacyRedactionsHelp — Wed., May 1, 12:44:57.1am ET