SEC Info℠  
    Home      Search      My Interests      Help      Sign In      Please Sign In

YIELD10 Bioscience, Inc. – ‘10-Q’ for 9/30/22 – ‘EX-101.DEF’

On:  Monday, 11/14/22, at 4:14pm ET   ·   For:  9/30/22   ·   Accession #:  1121702-22-49   ·   File #:  1-33133

Previous ‘10-Q’:  ‘10-Q’ on 8/10/22 for 6/30/22   ·   Next:  ‘10-Q’ on 5/12/23 for 3/31/23   ·   Latest:  ‘10-Q’ on 11/14/23 for 9/30/23   ·   3 References:   

Find Words in Filings emoji
 		  in    Show  and   ↓Hints

  As Of               Filer                 Filing    For·On·As Docs:Size

11/14/22  YIELD10 Bioscience, Inc.          10-Q        9/30/22   60:4.8M

Quarterly Report   —   Form 10-Q

Filing Table of Contents

Document/Exhibit                   Description                      Pages   Size 

 1: 10-Q        Quarterly Report                                    HTML   1.02M 
 2: EX-31.1     Certification -- §302 - SOA'02                      HTML     21K 
 3: EX-31.2     Certification -- §302 - SOA'02                      HTML     21K 
 4: EX-32.1     Certification -- §906 - SOA'02                      HTML     19K 
10: R1          Cover                                               HTML     69K 
11: R2          Condensed Consolidated Statements of Operations     HTML     85K 
                Unaudited                                                        
12: R3          Condensed Consolidated Balance Sheets Unaudited     HTML    112K 
13: R4          Condensed Consolidated Balance Sheets               HTML     35K 
                (Parenthetical)                                                  
14: R5          Condensed Consolidated Statements of Comprehensive  HTML     44K 
                Loss Unaudited                                                   
15: R6          Condensed Consolidated Statements of Cash Flows     HTML     89K 
                Unaudited                                                        
16: R7          Condensed Consolidated Statements of Stockholders'  HTML     81K 
                Equity                                                           
17: R8          Accounting Policies                                 HTML     51K 
18: R9          Nature of Business and Basis of Presentation        HTML     32K 
19: R10         Basic and Diluted Net Loss Per Share                HTML     28K 
20: R11         Investments                                         HTML     37K 
21: R12         Fair Value Measurements                             HTML     50K 
22: R13         Accrued Expenses                                    HTML     27K 
23: R14         Stock-Based Compensation                            HTML     37K 
24: R15         Leases                                              HTML     46K 
25: R16         Commitments and Contingencies                       HTML     27K 
26: R17         License Agreements                                  HTML     21K 
27: R18         Geographic Information                              HTML     42K 
28: R19         Capital Stock and Warrants                          HTML     42K 
29: R20         Accounting Policies (Policies)                      HTML     93K 
30: R21         Accounting Policies (Tables)                        HTML     37K 
31: R22         Basic and Diluted Net Loss Per Share (Tables)       HTML     26K 
32: R23         Investments (Tables)                                HTML     36K 
33: R24         Fair Value Measurements (Tables)                    HTML     47K 
34: R25         Accrued Expenses (Tables)                           HTML     26K 
35: R26         Stock-Based Compensation (Tables)                   HTML     35K 
36: R27         Leases (Tables)                                     HTML     44K 
37: R28         Geographic Information (Tables)                     HTML     39K 
38: R29         Capital Stock and Warrants (Tables)                 HTML     43K 
39: R30         ACCOUNTING POLICIES - Additional Information        HTML     35K 
                (Details)                                                        
40: R31         ACCOUNTING POLICIES - Schedule of Cash And          HTML     27K 
                Restricted Cash (Details)                                        
41: R32         Nature of Business and Basis of Presentation        HTML     20K 
                (Details)                                                        
42: R33         Basic and Diluted Net Loss Per Share (Details)      HTML     27K 
43: R34         Investments (Details)                               HTML     30K 
44: R35         FAIR VALUE MEASUREMENTS - Assets and Liabilities    HTML     45K 
                Measured at Fair Value on a Recurring Basis                      
                (Details)                                                        
45: R36         Accrued Expenses (Details)                          HTML     29K 
46: R37         Stock-Based Compensation (Details)                  HTML     82K 
47: R38         Stock-Based Compensation - RSU Activity (Details)   HTML     28K 
48: R39         LEASES - Narrative (Details)                        HTML     31K 
49: R40         LEASES - Maturity Analysis of Lease Liabilities     HTML     38K 
                (Details)                                                        
50: R41         LEASES - Quantitative Disclosure of Lease Costs     HTML     33K 
                (Details)                                                        
51: R42         Commitments and Contingencies (Details)             HTML     32K 
52: R43         Geographic Information (Details)                    HTML     31K 
53: R44         CAPITAL STOCK AND WARRANTS - Narrative (Details)    HTML     39K 
54: R45         CAPITAL STOCK AND WARRANTS - Schedule Of Warrants   HTML     34K 
                Issuance (Details)                                               
55: R46         CAPITAL STOCK AND WARRANTS - Schedule of Common     HTML     25K 
                Stock Reserved For Future Issuance (Details)                     
58: XML         IDEA XML File -- Filing Summary                      XML    103K 
56: XML         XBRL Instance -- yten-20220930_htm                   XML   1.11M 
57: EXCEL       IDEA Workbook of Financial Reports                  XLSX    100K 
 6: EX-101.CAL  XBRL Calculations -- yten-20220930_cal               XML    135K 
 7: EX-101.DEF  XBRL Definitions -- yten-20220930_def                XML    311K 
 8: EX-101.LAB  XBRL Labels -- yten-20220930_lab                     XML   1.05M 
 9: EX-101.PRE  XBRL Presentations -- yten-20220930_pre              XML    584K 
 5: EX-101.SCH  XBRL Schema -- yten-20220930                         XSD     96K 
59: JSON        XBRL Instance as JSON Data -- MetaLinks              296±   448K 
60: ZIP         XBRL Zipped Folder -- 0001121702-22-000049-xbrl      Zip    259K

‘EX-101.DEF’   —   XBRL Definitions — yten-20220930_def

This Exhibit is an XBRL XML File.

                                                                                                                                                                                
<?xml version="1.0" encoding="windows-1252"?>
<!-- XBRL Document Created with Wdesk from Workiva -->
<!-- Copyright 2022 Workiva -->
<!-- r:d1317484-fb72-4a35-a936-0de0909d8212,g:44a95c54-f659-440a-9ac9-343c03dbcc39 -->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
<link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
<link:roleRef roleURI="http://www.yield10bio.com/role/CONDENSEDCONSOLIDATEDSTATEMENTSOFOPERATIONSUNAUDITED" xlink:type="simple" xlink:href="yten-20220930.xsd#CONDENSEDCONSOLIDATEDSTATEMENTSOFOPERATIONSUNAUDITED"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/CONDENSEDCONSOLIDATEDSTATEMENTSOFOPERATIONSUNAUDITED" xlink:type="extended" id="i4ef982935b7048b3882d3842542f7f96_CONDENSEDCONSOLIDATEDSTATEMENTSOFOPERATIONSUNAUDITED">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_efd8e375-9536-4107-bc78-8c1ec766243d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenuesAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_RevenuesAbstract_efd8e375-9536-4107-bc78-8c1ec766243d" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_b1141be8-db27-447e-a9af-6b8329cebbc3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_efd8e375-9536-4107-bc78-8c1ec766243d" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_b1141be8-db27-447e-a9af-6b8329cebbc3" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpensesAbstract_057da9da-6396-4fba-a572-3a857150149f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostsAndExpensesAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_CostsAndExpensesAbstract_057da9da-6396-4fba-a572-3a857150149f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_CostOfRevenueResearchAndDevelopmentExpenses_4db2eac8-5e89-4cde-957a-5501eae7f493" xlink:href="yten-20220930.xsd#yten_CostOfRevenueResearchAndDevelopmentExpenses"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_057da9da-6396-4fba-a572-3a857150149f" xlink:to="loc_yten_CostOfRevenueResearchAndDevelopmentExpenses_4db2eac8-5e89-4cde-957a-5501eae7f493" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_fa521cae-cdd5-4a9f-aab0-9b86b295c68a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_057da9da-6396-4fba-a572-3a857150149f" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_fa521cae-cdd5-4a9f-aab0-9b86b295c68a" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_46a90577-5784-4e30-a543-cdba13cc5531" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostsAndExpenses"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_057da9da-6396-4fba-a572-3a857150149f" xlink:to="loc_us-gaap_CostsAndExpenses_46a90577-5784-4e30-a543-cdba13cc5531" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_54cc47b1-fd6f-4f08-a1ac-64f71b7b8cf0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_OperatingIncomeLoss_54cc47b1-fd6f-4f08-a1ac-64f71b7b8cf0" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpenseAbstract_af2da798-e35b-4057-b7c0-a3f547c92cbc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NonoperatingIncomeExpenseAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_NonoperatingIncomeExpenseAbstract_af2da798-e35b-4057-b7c0-a3f547c92cbc" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_GainOnRelatedPartyInvestment_173a842d-aa9e-4275-81f0-149a56444220" xlink:href="yten-20220930.xsd#yten_GainOnRelatedPartyInvestment"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_af2da798-e35b-4057-b7c0-a3f547c92cbc" xlink:to="loc_yten_GainOnRelatedPartyInvestment_173a842d-aa9e-4275-81f0-149a56444220" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_32097f72-9ea0-47ea-a933-c1fcd0645303" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_af2da798-e35b-4057-b7c0-a3f547c92cbc" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_32097f72-9ea0-47ea-a933-c1fcd0645303" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_6df722d6-78f9-4d15-b982-ca2db4a4bd29" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NonoperatingIncomeExpense"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_af2da798-e35b-4057-b7c0-a3f547c92cbc" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_6df722d6-78f9-4d15-b982-ca2db4a4bd29" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_8c5cc3f6-b162-489d-9c2b-8f43f3f4f4f6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_8c5cc3f6-b162-489d-9c2b-8f43f3f4f4f6" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_4ab8f837-e088-4963-89e7-7272c7d787f0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_4ab8f837-e088-4963-89e7-7272c7d787f0" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_4663c25c-ba65-45ae-9d9a-1a2f2e5f4946" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_NetIncomeLoss_4663c25c-ba65-45ae-9d9a-1a2f2e5f4946" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_10c49857-b8e6-4564-9533-ce2713cd434c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EarningsPerShareBasic"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_EarningsPerShareBasic_10c49857-b8e6-4564-9533-ce2713cd434c" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_2f01afd7-e605-4b8f-9b38-3954b3e31a9f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EarningsPerShareDiluted"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_EarningsPerShareDiluted_2f01afd7-e605-4b8f-9b38-3954b3e31a9f" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_c8434c7a-3c56-4f36-8a42-1f1dbc84e1a3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_c8434c7a-3c56-4f36-8a42-1f1dbc84e1a3" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_3d1f41d6-e050-4963-b87f-2f4365c84ad7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_c8434c7a-3c56-4f36-8a42-1f1dbc84e1a3" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_3d1f41d6-e050-4963-b87f-2f4365c84ad7" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_67e003ad-02ac-467d-aa27-047595bea4d8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_c8434c7a-3c56-4f36-8a42-1f1dbc84e1a3" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_67e003ad-02ac-467d-aa27-047595bea4d8" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_2114baaa-0e7b-41f3-8be7-c4deed2ed29b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_41a6f0f3-5ead-4ba9-b9b4-053a17dfab13" xlink:to="loc_us-gaap_StatementTable_2114baaa-0e7b-41f3-8be7-c4deed2ed29b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_7a0b259f-894e-4baa-8c15-683f13b21913" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ProductOrServiceAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_2114baaa-0e7b-41f3-8be7-c4deed2ed29b" xlink:to="loc_srt_ProductOrServiceAxis_7a0b259f-894e-4baa-8c15-683f13b21913" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_7a0b259f-894e-4baa-8c15-683f13b21913_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ProductsAndServicesDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_7a0b259f-894e-4baa-8c15-683f13b21913" xlink:to="loc_srt_ProductsAndServicesDomain_7a0b259f-894e-4baa-8c15-683f13b21913_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_62393211-c8ae-49d0-9ac4-b760fe5cc738" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_ProductsAndServicesDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_7a0b259f-894e-4baa-8c15-683f13b21913" xlink:to="loc_srt_ProductsAndServicesDomain_62393211-c8ae-49d0-9ac4-b760fe5cc738" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrantMember_928a541b-c357-407c-8087-831369600a04" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GrantMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_62393211-c8ae-49d0-9ac4-b760fe5cc738" xlink:to="loc_us-gaap_GrantMember_928a541b-c357-407c-8087-831369600a04" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/CONDENSEDCONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSEQUITY" xlink:type="simple" xlink:href="yten-20220930.xsd#CONDENSEDCONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSEQUITY"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/CONDENSEDCONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSEQUITY" xlink:type="extended" id="ie087bbbaf3d24a6685dfdabfe904f1a8_CONDENSEDCONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSEQUITY">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_7f72dfae-e8c1-4d57-a6b1-992a79f93ef5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_7f72dfae-e8c1-4d57-a6b1-992a79f93ef5" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_546ac94f-5849-4880-8027-223ec666cd4e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharesOutstanding"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_SharesOutstanding_546ac94f-5849-4880-8027-223ec666cd4e" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_e06a21fa-a615-474f-9b53-7ea56b257738" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_StockholdersEquity_e06a21fa-a615-474f-9b53-7ea56b257738" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_421038e0-c49b-4f26-b34a-8bfab3f3167b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_421038e0-c49b-4f26-b34a-8bfab3f3167b" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeBenefitPlan_46e1e7b4-948d-4757-b60a-1c3177e502a3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeBenefitPlan"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeBenefitPlan_46e1e7b4-948d-4757-b60a-1c3177e502a3" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeBenefitPlan_8cf07e95-e1d5-45f5-bec7-64cce9916ab0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeBenefitPlan"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeBenefitPlan_8cf07e95-e1d5-45f5-bec7-64cce9916ab0" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_d6eff70d-1ccc-484d-9c08-9624b9f7bff4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_d6eff70d-1ccc-484d-9c08-9624b9f7bff4" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_cb99e5d4-a133-4568-b423-ccc26aa5aecf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_cb99e5d4-a133-4568-b423-ccc26aa5aecf" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_e29e472e-d827-42e8-8dfa-4a371e8ae49a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_e29e472e-d827-42e8-8dfa-4a371e8ae49a" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_8c7cae47-3065-4063-a4f7-a0bae446687d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_8c7cae47-3065-4063-a4f7-a0bae446687d" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_3ca7c75b-bd62-4648-8d13-e1d7452ecc2b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_3ca7c75b-bd62-4648-8d13-e1d7452ecc2b" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_42dae7d4-85ba-4888-8ddc-058fae93200d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_42dae7d4-85ba-4888-8ddc-058fae93200d" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_1640cf38-9ad4-4e6f-8e35-f5ca2368eb51" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_1640cf38-9ad4-4e6f-8e35-f5ca2368eb51" xlink:type="arc" order="12"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_75cdddf4-2f56-4af9-8211-f9cbe806a584" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_b49d8ba5-ee23-4af7-aad0-e99297df0211" xlink:to="loc_us-gaap_NetIncomeLoss_75cdddf4-2f56-4af9-8211-f9cbe806a584" xlink:type="arc" order="13"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_e68b6f0d-756e-4087-96ce-99971ccfdf57" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharesOutstanding"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_b5f43427-aadf-408e-b381-2f520aa29057" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_51f21bf1-4516-4c69-8f44-a21ed9d418fb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_7f72dfae-e8c1-4d57-a6b1-992a79f93ef5" xlink:to="loc_us-gaap_StatementTable_51f21bf1-4516-4c69-8f44-a21ed9d418fb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_3b7d994e-64dd-40f0-8a52-7199fb745b05" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_51f21bf1-4516-4c69-8f44-a21ed9d418fb" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_3b7d994e-64dd-40f0-8a52-7199fb745b05" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_3b7d994e-64dd-40f0-8a52-7199fb745b05_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_3b7d994e-64dd-40f0-8a52-7199fb745b05" xlink:to="loc_us-gaap_EquityComponentDomain_3b7d994e-64dd-40f0-8a52-7199fb745b05_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_64b48b95-df2f-4ecf-92c3-ac07f354c7e0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_3b7d994e-64dd-40f0-8a52-7199fb745b05" xlink:to="loc_us-gaap_EquityComponentDomain_64b48b95-df2f-4ecf-92c3-ac07f354c7e0" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_3abe2706-d4f0-46f7-801e-db1c01281409" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_64b48b95-df2f-4ecf-92c3-ac07f354c7e0" xlink:to="loc_us-gaap_CommonStockMember_3abe2706-d4f0-46f7-801e-db1c01281409" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_ed111167-6c8d-4faf-9f79-0b9dcbf4bf7d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_64b48b95-df2f-4ecf-92c3-ac07f354c7e0" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_ed111167-6c8d-4faf-9f79-0b9dcbf4bf7d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_7095cbb4-2522-4a62-9e2d-fe84d0f038ad" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_64b48b95-df2f-4ecf-92c3-ac07f354c7e0" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_7095cbb4-2522-4a62-9e2d-fe84d0f038ad" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_41d6f594-8a9c-42ad-9b0e-ab9acd9c01d1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_64b48b95-df2f-4ecf-92c3-ac07f354c7e0" xlink:to="loc_us-gaap_RetainedEarningsMember_41d6f594-8a9c-42ad-9b0e-ab9acd9c01d1" xlink:type="arc" order="3"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/ACCOUNTINGPOLICIESAdditionalInformationDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#ACCOUNTINGPOLICIESAdditionalInformationDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/ACCOUNTINGPOLICIESAdditionalInformationDetails" xlink:type="extended" id="icbc51bbe219f41039139706725c84f42_ACCOUNTINGPOLICIESAdditionalInformationDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_849ad5c3-931d-4e37-9a96-7db7b01464b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalents_2eee1ec5-1f62-4ade-be46-c13decb49eaf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RestrictedCashAndCashEquivalents"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_849ad5c3-931d-4e37-9a96-7db7b01464b5" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalents_2eee1ec5-1f62-4ade-be46-c13decb49eaf" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_AccountsReceivableAfterAllowanceForCreditLossCurrentAndUnbilledContractsReceivable_14a77073-24d0-4827-8bff-7a28fc514602" xlink:href="yten-20220930.xsd#yten_AccountsReceivableAfterAllowanceForCreditLossCurrentAndUnbilledContractsReceivable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_849ad5c3-931d-4e37-9a96-7db7b01464b5" xlink:to="loc_yten_AccountsReceivableAfterAllowanceForCreditLossCurrentAndUnbilledContractsReceivable_14a77073-24d0-4827-8bff-7a28fc514602" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_f0e96e0f-cb51-489f-bfd3-c76cb10813cb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NumberOfOperatingSegments"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_849ad5c3-931d-4e37-9a96-7db7b01464b5" xlink:to="loc_us-gaap_NumberOfOperatingSegments_f0e96e0f-cb51-489f-bfd3-c76cb10813cb" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_9998e3ab-e448-4975-8b36-79c6eae41b42" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_849ad5c3-931d-4e37-9a96-7db7b01464b5" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_9998e3ab-e448-4975-8b36-79c6eae41b42" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_7f6d5710-4ac7-45c7-af90-e48b46a12989" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConcentrationRiskTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_849ad5c3-931d-4e37-9a96-7db7b01464b5" xlink:to="loc_us-gaap_ConcentrationRiskTable_7f6d5710-4ac7-45c7-af90-e48b46a12989" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_323952f1-2a91-4212-8b28-a49f5ebe9921" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_7f6d5710-4ac7-45c7-af90-e48b46a12989" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_323952f1-2a91-4212-8b28-a49f5ebe9921" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_323952f1-2a91-4212-8b28-a49f5ebe9921_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_323952f1-2a91-4212-8b28-a49f5ebe9921" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_323952f1-2a91-4212-8b28-a49f5ebe9921_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_7a6e5f6e-e691-4a54-adf7-700528636a1f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_323952f1-2a91-4212-8b28-a49f5ebe9921" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_7a6e5f6e-e691-4a54-adf7-700528636a1f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentMember_2e560707-2ea9-4aaa-b788-ed687ac6f4c8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquipmentMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_7a6e5f6e-e691-4a54-adf7-700528636a1f" xlink:to="loc_us-gaap_EquipmentMember_2e560707-2ea9-4aaa-b788-ed687ac6f4c8" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_b6801ace-d993-4a6d-9a98-efb985cfb981" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FurnitureAndFixturesMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_7a6e5f6e-e691-4a54-adf7-700528636a1f" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_b6801ace-d993-4a6d-9a98-efb985cfb981" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerSoftwareIntangibleAssetMember_1ef4fac3-af36-47b0-9ccd-3ceae2179589" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ComputerSoftwareIntangibleAssetMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_7a6e5f6e-e691-4a54-adf7-700528636a1f" xlink:to="loc_us-gaap_ComputerSoftwareIntangibleAssetMember_1ef4fac3-af36-47b0-9ccd-3ceae2179589" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_c2a8198a-a375-4a6c-96bb-c639b0ff5108" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MajorCustomersAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_7f6d5710-4ac7-45c7-af90-e48b46a12989" xlink:to="loc_srt_MajorCustomersAxis_c2a8198a-a375-4a6c-96bb-c639b0ff5108" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_c2a8198a-a375-4a6c-96bb-c639b0ff5108_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_NameOfMajorCustomerDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_c2a8198a-a375-4a6c-96bb-c639b0ff5108" xlink:to="loc_srt_NameOfMajorCustomerDomain_c2a8198a-a375-4a6c-96bb-c639b0ff5108_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_69c0525e-804d-4440-8673-ef5758d0898a" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_NameOfMajorCustomerDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_c2a8198a-a375-4a6c-96bb-c639b0ff5108" xlink:to="loc_srt_NameOfMajorCustomerDomain_69c0525e-804d-4440-8673-ef5758d0898a" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_MichiganStateUniversityMember_4765ba7d-4881-4584-b807-ce079c20c250" xlink:href="yten-20220930.xsd#yten_MichiganStateUniversityMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_69c0525e-804d-4440-8673-ef5758d0898a" xlink:to="loc_yten_MichiganStateUniversityMember_4765ba7d-4881-4584-b807-ce079c20c250" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/BASICANDDILUTEDNETLOSSPERSHAREDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#BASICANDDILUTEDNETLOSSPERSHAREDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/BASICANDDILUTEDNETLOSSPERSHAREDetails" xlink:type="extended" id="i3c502b5539ca4c47ba4e0d4b2c04d466_BASICANDDILUTEDNETLOSSPERSHAREDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_d53d36ea-7509-479c-b138-c4480ec41db2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_ab11bd65-e59f-4ec2-b295-ec89ff676773" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_d53d36ea-7509-479c-b138-c4480ec41db2" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_ab11bd65-e59f-4ec2-b295-ec89ff676773" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_1819ea7c-4ff0-4a78-9ca9-a2d35e31e65d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_d53d36ea-7509-479c-b138-c4480ec41db2" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_1819ea7c-4ff0-4a78-9ca9-a2d35e31e65d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_478f8585-af1f-4bff-b36e-bfc5ace966b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_1819ea7c-4ff0-4a78-9ca9-a2d35e31e65d" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_478f8585-af1f-4bff-b36e-bfc5ace966b3" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_478f8585-af1f-4bff-b36e-bfc5ace966b3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_478f8585-af1f-4bff-b36e-bfc5ace966b3" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_478f8585-af1f-4bff-b36e-bfc5ace966b3_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_3265837b-e1e3-46c6-98bf-d5bf921e1c9f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_478f8585-af1f-4bff-b36e-bfc5ace966b3" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_3265837b-e1e3-46c6-98bf-d5bf921e1c9f" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_EmployeeAndDirectorsStockOptionsMember_338ea67d-8992-4db3-b650-b025798b7b44" xlink:href="yten-20220930.xsd#yten_EmployeeAndDirectorsStockOptionsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_3265837b-e1e3-46c6-98bf-d5bf921e1c9f" xlink:to="loc_yten_EmployeeAndDirectorsStockOptionsMember_338ea67d-8992-4db3-b650-b025798b7b44" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_390fc221-d6c1-4a82-953f-724e4dec63c4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_3265837b-e1e3-46c6-98bf-d5bf921e1c9f" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_390fc221-d6c1-4a82-953f-724e4dec63c4" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_WarrantMember_b0ffcf6c-21a2-48dc-bd9c-67ef52d685b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WarrantMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_3265837b-e1e3-46c6-98bf-d5bf921e1c9f" xlink:to="loc_us-gaap_WarrantMember_b0ffcf6c-21a2-48dc-bd9c-67ef52d685b5" xlink:type="arc" order="2"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/INVESTMENTSDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#INVESTMENTSDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/INVESTMENTSDetails" xlink:type="extended" id="idcb59e77627d4823b90410b316d1be3a_INVESTMENTSDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_7b30eef3-7b5d-4ecc-a9ed-e20149f03296" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_33d7d0fe-b105-4ae9-839f-1d9f9afe38d3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_7b30eef3-7b5d-4ecc-a9ed-e20149f03296" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_33d7d0fe-b105-4ae9-839f-1d9f9afe38d3" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_d0ed35f6-64c3-4fb1-a776-953c3022e451" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_7b30eef3-7b5d-4ecc-a9ed-e20149f03296" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_d0ed35f6-64c3-4fb1-a776-953c3022e451" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_69c73b26-197c-4196-9a9d-ae47967997b0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_7b30eef3-7b5d-4ecc-a9ed-e20149f03296" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_69c73b26-197c-4196-9a9d-ae47967997b0" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_15c0def8-32e0-4969-9d0c-275d996bce2f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_7b30eef3-7b5d-4ecc-a9ed-e20149f03296" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_15c0def8-32e0-4969-9d0c-275d996bce2f" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_e39a2f76-55fd-4fc6-ac35-cbae5c11e963" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_7b30eef3-7b5d-4ecc-a9ed-e20149f03296" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_e39a2f76-55fd-4fc6-ac35-cbae5c11e963" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_257fed2b-b119-4bdc-b18d-3544bddc275b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_e39a2f76-55fd-4fc6-ac35-cbae5c11e963" xlink:to="loc_us-gaap_FinancialInstrumentAxis_257fed2b-b119-4bdc-b18d-3544bddc275b" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_257fed2b-b119-4bdc-b18d-3544bddc275b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_257fed2b-b119-4bdc-b18d-3544bddc275b" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_257fed2b-b119-4bdc-b18d-3544bddc275b_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_cc58c6dd-65c8-446f-9d69-247df75a3afe" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_257fed2b-b119-4bdc-b18d-3544bddc275b" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_cc58c6dd-65c8-446f-9d69-247df75a3afe" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryAndGovernmentMember_03eb7a62-2ed4-4e9f-81c2-7cb8b55f8342" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USTreasuryAndGovernmentMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_cc58c6dd-65c8-446f-9d69-247df75a3afe" xlink:to="loc_us-gaap_USTreasuryAndGovernmentMember_03eb7a62-2ed4-4e9f-81c2-7cb8b55f8342" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/FAIRVALUEMEASUREMENTSAssetsandLiabilitiesMeasuredatFairValueonaRecurringBasisDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#FAIRVALUEMEASUREMENTSAssetsandLiabilitiesMeasuredatFairValueonaRecurringBasisDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/FAIRVALUEMEASUREMENTSAssetsandLiabilitiesMeasuredatFairValueonaRecurringBasisDetails" xlink:type="extended" id="ifb3469f257cd495780aba0397761fbb7_FAIRVALUEMEASUREMENTSAssetsandLiabilitiesMeasuredatFairValueonaRecurringBasisDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6ab1946-ae2b-436f-b799-7f299729e684" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_cf8e6cd6-1f6e-4941-a10b-3f0f5e9a1861" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6ab1946-ae2b-436f-b799-7f299729e684" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_cf8e6cd6-1f6e-4941-a10b-3f0f5e9a1861" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_cb995357-11c6-4a8a-9233-866986a2f386" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6ab1946-ae2b-436f-b799-7f299729e684" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_cb995357-11c6-4a8a-9233-866986a2f386" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_2328a596-8f5a-48f1-982c-163b93af2614" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsFairValueDisclosure"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6ab1946-ae2b-436f-b799-7f299729e684" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_2328a596-8f5a-48f1-982c-163b93af2614" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_2d41bcc9-b897-4e28-b91a-3cd0905c1ca0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d6ab1946-ae2b-436f-b799-7f299729e684" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_2d41bcc9-b897-4e28-b91a-3cd0905c1ca0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_9de8f6b3-b97c-4716-9f00-6dc65b04753b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_2d41bcc9-b897-4e28-b91a-3cd0905c1ca0" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_9de8f6b3-b97c-4716-9f00-6dc65b04753b" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_9de8f6b3-b97c-4716-9f00-6dc65b04753b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_9de8f6b3-b97c-4716-9f00-6dc65b04753b" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_9de8f6b3-b97c-4716-9f00-6dc65b04753b_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_677b106a-d93f-45cd-88c5-5313cc0f6604" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_9de8f6b3-b97c-4716-9f00-6dc65b04753b" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_677b106a-d93f-45cd-88c5-5313cc0f6604" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_413d112a-fb6e-4cf9-b7fe-3052347d2f2e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_677b106a-d93f-45cd-88c5-5313cc0f6604" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_413d112a-fb6e-4cf9-b7fe-3052347d2f2e" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_7f5d9ca6-c66e-4493-b7e5-c2bfaac73ca6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_2d41bcc9-b897-4e28-b91a-3cd0905c1ca0" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_7f5d9ca6-c66e-4493-b7e5-c2bfaac73ca6" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_7f5d9ca6-c66e-4493-b7e5-c2bfaac73ca6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_7f5d9ca6-c66e-4493-b7e5-c2bfaac73ca6" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_7f5d9ca6-c66e-4493-b7e5-c2bfaac73ca6_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6e8b1c68-52f5-4944-bde5-cd283d9f58c8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_7f5d9ca6-c66e-4493-b7e5-c2bfaac73ca6" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6e8b1c68-52f5-4944-bde5-cd283d9f58c8" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_09fdfbe9-1089-4f32-b4a5-284ff6583870" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel1Member"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6e8b1c68-52f5-4944-bde5-cd283d9f58c8" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_09fdfbe9-1089-4f32-b4a5-284ff6583870" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_2c71e546-0399-43ac-a9e8-d0097f2ab39e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel2Member"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6e8b1c68-52f5-4944-bde5-cd283d9f58c8" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_2c71e546-0399-43ac-a9e8-d0097f2ab39e" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_9b349ba0-f48e-49b5-9849-0d25c0f5a335" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel3Member"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6e8b1c68-52f5-4944-bde5-cd283d9f58c8" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_9b349ba0-f48e-49b5-9849-0d25c0f5a335" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAxis_4d13fa40-e15f-4299-ba5b-3a8bcbd1150a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_2d41bcc9-b897-4e28-b91a-3cd0905c1ca0" xlink:to="loc_us-gaap_CashAndCashEquivalentsAxis_4d13fa40-e15f-4299-ba5b-3a8bcbd1150a" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_4d13fa40-e15f-4299-ba5b-3a8bcbd1150a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_4d13fa40-e15f-4299-ba5b-3a8bcbd1150a" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_4d13fa40-e15f-4299-ba5b-3a8bcbd1150a_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ffc9b56a-3757-4f49-9b69-4e1af22b4c18" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_4d13fa40-e15f-4299-ba5b-3a8bcbd1150a" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ffc9b56a-3757-4f49-9b69-4e1af22b4c18" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_d4652d1a-94e8-47ed-8fc6-3c0c4ef7cf51" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MoneyMarketFundsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ffc9b56a-3757-4f49-9b69-4e1af22b4c18" xlink:to="loc_us-gaap_MoneyMarketFundsMember_d4652d1a-94e8-47ed-8fc6-3c0c4ef7cf51" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentCorporationsAndAgenciesSecuritiesMember_cdffa81d-abb3-4203-be0f-4c93e3410a4d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USGovernmentCorporationsAndAgenciesSecuritiesMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_ffc9b56a-3757-4f49-9b69-4e1af22b4c18" xlink:to="loc_us-gaap_USGovernmentCorporationsAndAgenciesSecuritiesMember_cdffa81d-abb3-4203-be0f-4c93e3410a4d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_22849b92-c976-497d-919e-c9fda176d8d7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_2d41bcc9-b897-4e28-b91a-3cd0905c1ca0" xlink:to="loc_us-gaap_FinancialInstrumentAxis_22849b92-c976-497d-919e-c9fda176d8d7" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_22849b92-c976-497d-919e-c9fda176d8d7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_22849b92-c976-497d-919e-c9fda176d8d7" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_22849b92-c976-497d-919e-c9fda176d8d7_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_781e9a93-164a-4a26-b230-8371e2bc7e2d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_22849b92-c976-497d-919e-c9fda176d8d7" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_781e9a93-164a-4a26-b230-8371e2bc7e2d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentCorporationsAndAgenciesSecuritiesMember_c105c901-2a38-450f-a8ac-0843f3dbf8d5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USGovernmentCorporationsAndAgenciesSecuritiesMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_781e9a93-164a-4a26-b230-8371e2bc7e2d" xlink:to="loc_us-gaap_USGovernmentCorporationsAndAgenciesSecuritiesMember_c105c901-2a38-450f-a8ac-0843f3dbf8d5" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/STOCKBASEDCOMPENSATIONDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#STOCKBASEDCOMPENSATIONDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/STOCKBASEDCOMPENSATIONDetails" xlink:type="extended" id="iad66f1950adb4f768ba428bf6e401024_STOCKBASEDCOMPENSATIONDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_a073f8dd-5562-4f5a-a9ed-55e77ccad476" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensation"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ShareBasedCompensation_a073f8dd-5562-4f5a-a9ed-55e77ccad476" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_27c39d13-82a4-4c39-a328-ba855f394b97" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_27c39d13-82a4-4c39-a328-ba855f394b97" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_b0b9af4c-af47-434b-8fcb-f1cd9894640a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_b0b9af4c-af47-434b-8fcb-f1cd9894640a" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_3d54ef02-7548-45ab-aa78-bdd00963f86f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_3d54ef02-7548-45ab-aa78-bdd00963f86f" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_abdfadc7-76aa-4527-bd54-bbbc94303ece" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_3d54ef02-7548-45ab-aa78-bdd00963f86f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_abdfadc7-76aa-4527-bd54-bbbc94303ece" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_1176af2e-6472-4da5-9e0a-ffdac1485040" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_3d54ef02-7548-45ab-aa78-bdd00963f86f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_1176af2e-6472-4da5-9e0a-ffdac1485040" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_eb86685f-e343-4a37-a42c-29ba37e69430" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_3d54ef02-7548-45ab-aa78-bdd00963f86f" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_eb86685f-e343-4a37-a42c-29ba37e69430" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_25a48219-cb9c-43cf-be5c-dafd4bf6d154" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_3d54ef02-7548-45ab-aa78-bdd00963f86f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_25a48219-cb9c-43cf-be5c-dafd4bf6d154" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod_25e4befc-ebd9-41fb-b892-9fcab0466b97" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_3d54ef02-7548-45ab-aa78-bdd00963f86f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod_25e4befc-ebd9-41fb-b892-9fcab0466b97" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_118188ce-6bb2-4f6c-850e-ac1a5ce77ee9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_847b5b30-a70e-4eb5-93c4-1daef7260886" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_847b5b30-a70e-4eb5-93c4-1daef7260886" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_72f09f76-c3a0-40b5-aa77-712872d66e56" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_72f09f76-c3a0-40b5-aa77-712872d66e56" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_beacb744-6c77-4e2a-93fc-4be130b36277" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_72f09f76-c3a0-40b5-aa77-712872d66e56" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_beacb744-6c77-4e2a-93fc-4be130b36277" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_70314e61-7fa2-4a99-aab9-9f5ae355146b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_72f09f76-c3a0-40b5-aa77-712872d66e56" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_70314e61-7fa2-4a99-aab9-9f5ae355146b" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_5abc6726-5de4-4380-a12f-210662ebef8e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_72f09f76-c3a0-40b5-aa77-712872d66e56" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_5abc6726-5de4-4380-a12f-210662ebef8e" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_61aaa591-13f8-4fb1-a37d-de3bcebe571e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_72f09f76-c3a0-40b5-aa77-712872d66e56" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_61aaa591-13f8-4fb1-a37d-de3bcebe571e" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice_19acad4c-fa77-4333-b4be-2790f70f442e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_72f09f76-c3a0-40b5-aa77-712872d66e56" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice_19acad4c-fa77-4333-b4be-2790f70f442e" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_35e7890d-e8df-4e89-bd4f-a1426d374670" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_9880480e-9cd2-438b-9d2b-66ca9e56c7ef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_9880480e-9cd2-438b-9d2b-66ca9e56c7ef" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized_f1c8778a-7267-47b9-b0d4-22a734f646f1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized_f1c8778a-7267-47b9-b0d4-22a734f646f1" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_ShareBasedCompensationArrangementByShareBasedPaymentAwardAdditionalSharesAuthorizedPercent_0d8f1017-de2c-48f8-82da-73a0c5e14243" xlink:href="yten-20220930.xsd#yten_ShareBasedCompensationArrangementByShareBasedPaymentAwardAdditionalSharesAuthorizedPercent"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_yten_ShareBasedCompensationArrangementByShareBasedPaymentAwardAdditionalSharesAuthorizedPercent_0d8f1017-de2c-48f8-82da-73a0c5e14243" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_dcc76633-fb35-4bff-8662-67b2a3627217" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_dcc76633-fb35-4bff-8662-67b2a3627217" xlink:type="arc" order="9"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_24e1c548-c2c9-45cf-86eb-b41c31d3ba29" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_24e1c548-c2c9-45cf-86eb-b41c31d3ba29" xlink:type="arc" order="10"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation_1bb80775-6666-40b8-8d0d-9115e3e97d5f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation_1bb80775-6666-40b8-8d0d-9115e3e97d5f" xlink:type="arc" order="11"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_PaymentTaxWithholdingShareBasedPaymentArrangementExcludingEmployerAmount_6c05e97f-0286-4809-8a6e-4a9ea9012eb8" xlink:href="yten-20220930.xsd#yten_PaymentTaxWithholdingShareBasedPaymentArrangementExcludingEmployerAmount"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_yten_PaymentTaxWithholdingShareBasedPaymentArrangementExcludingEmployerAmount_6c05e97f-0286-4809-8a6e-4a9ea9012eb8" xlink:type="arc" order="12"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_92d82fc7-9412-40f0-92f8-fa1d52eccdb0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_25254fd8-7a8f-43ef-93f5-646bba841c2e" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_92d82fc7-9412-40f0-92f8-fa1d52eccdb0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_e7eb6082-dfa7-4fa4-aa98-139a901d9302" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AwardTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_92d82fc7-9412-40f0-92f8-fa1d52eccdb0" xlink:to="loc_us-gaap_AwardTypeAxis_e7eb6082-dfa7-4fa4-aa98-139a901d9302" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e7eb6082-dfa7-4fa4-aa98-139a901d9302_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_e7eb6082-dfa7-4fa4-aa98-139a901d9302" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_e7eb6082-dfa7-4fa4-aa98-139a901d9302_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6d94df66-d8e6-477f-b744-b987f21454c0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_e7eb6082-dfa7-4fa4-aa98-139a901d9302" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6d94df66-d8e6-477f-b744-b987f21454c0" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_21d80c10-bd37-4c44-b56d-b8935d8c85b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeStockOptionMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6d94df66-d8e6-477f-b744-b987f21454c0" xlink:to="loc_us-gaap_EmployeeStockOptionMember_21d80c10-bd37-4c44-b56d-b8935d8c85b3" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_EmployeeAndDirectorsStockOptionsMember_c3146102-94b7-4927-9598-b9330c2f8342" xlink:href="yten-20220930.xsd#yten_EmployeeAndDirectorsStockOptionsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6d94df66-d8e6-477f-b744-b987f21454c0" xlink:to="loc_yten_EmployeeAndDirectorsStockOptionsMember_c3146102-94b7-4927-9598-b9330c2f8342" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_b2f6a083-2a9b-4c32-a49f-bac80a5877b0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6d94df66-d8e6-477f-b744-b987f21454c0" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_b2f6a083-2a9b-4c32-a49f-bac80a5877b0" xlink:type="arc" order="2"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/STOCKBASEDCOMPENSATIONRSUActivityDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#STOCKBASEDCOMPENSATIONRSUActivityDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/STOCKBASEDCOMPENSATIONRSUActivityDetails" xlink:type="extended" id="i3af63a825835414dbceac36a8f3f1b95_STOCKBASEDCOMPENSATIONRSUActivityDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_07a36d84-f597-4811-b0af-20886b69af6c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsAdditionalDisclosuresAbstract_ed4af4da-fb22-4a07-a44d-f120fcfd1ee6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsAdditionalDisclosuresAbstract"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_07a36d84-f597-4811-b0af-20886b69af6c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsAdditionalDisclosuresAbstract_ed4af4da-fb22-4a07-a44d-f120fcfd1ee6" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_4254d3cc-64cb-4601-9b1d-186e0e8fa5c4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsAdditionalDisclosuresAbstract_ed4af4da-fb22-4a07-a44d-f120fcfd1ee6" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_4254d3cc-64cb-4601-9b1d-186e0e8fa5c4" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_e7d37023-53f4-4f95-9f9f-f0d416c46120" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_4254d3cc-64cb-4601-9b1d-186e0e8fa5c4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_e7d37023-53f4-4f95-9f9f-f0d416c46120" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_a9dd7e33-eb6c-49a1-a673-a59378c3cfcb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_4254d3cc-64cb-4601-9b1d-186e0e8fa5c4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_a9dd7e33-eb6c-49a1-a673-a59378c3cfcb" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_c7688e05-331f-4977-8cc1-bf0c5f4970fd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_4254d3cc-64cb-4601-9b1d-186e0e8fa5c4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_c7688e05-331f-4977-8cc1-bf0c5f4970fd" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_10265a9a-662a-48d8-b4cc-a3c52f2b46f7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_2132287a-572d-44ad-8175-b5c3256ec1bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsAdditionalDisclosuresAbstract_ed4af4da-fb22-4a07-a44d-f120fcfd1ee6" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_2132287a-572d-44ad-8175-b5c3256ec1bb" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_80c37d90-58cc-4923-a472-0f04f35ef47b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_07a36d84-f597-4811-b0af-20886b69af6c" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_80c37d90-58cc-4923-a472-0f04f35ef47b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_7efb9128-a35c-4831-85f0-9ae3652573b7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AwardTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_80c37d90-58cc-4923-a472-0f04f35ef47b" xlink:to="loc_us-gaap_AwardTypeAxis_7efb9128-a35c-4831-85f0-9ae3652573b7" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_7efb9128-a35c-4831-85f0-9ae3652573b7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_7efb9128-a35c-4831-85f0-9ae3652573b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_7efb9128-a35c-4831-85f0-9ae3652573b7_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2485bb08-647e-4797-a3b1-ac7143f4cdb8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_7efb9128-a35c-4831-85f0-9ae3652573b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2485bb08-647e-4797-a3b1-ac7143f4cdb8" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_af3a10f4-9265-4544-89da-bb1afa1062c1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2485bb08-647e-4797-a3b1-ac7143f4cdb8" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_af3a10f4-9265-4544-89da-bb1afa1062c1" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/LEASESNarrativeDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#LEASESNarrativeDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/LEASESNarrativeDetails" xlink:type="extended" id="i1725d942822a449987109fdc6102ef71_LEASESNarrativeDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_79e0a9ac-acf9-4db4-9911-827d0307229a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AreaOfRealEstateProperty_741be294-01a1-4bd4-aea4-6b1bf0899998" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AreaOfRealEstateProperty"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_79e0a9ac-acf9-4db4-9911-827d0307229a" xlink:to="loc_us-gaap_AreaOfRealEstateProperty_741be294-01a1-4bd4-aea4-6b1bf0899998" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LettersOfCreditOutstandingAmount_e57c5371-0da4-4f56-88af-ae212efa5710" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LettersOfCreditOutstandingAmount"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_79e0a9ac-acf9-4db4-9911-827d0307229a" xlink:to="loc_us-gaap_LettersOfCreditOutstandingAmount_e57c5371-0da4-4f56-88af-ae212efa5710" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_LesseeOperatingSubleaseSubleasedArea_b75cf73f-12b3-4179-86e8-2672832951e6" xlink:href="yten-20220930.xsd#yten_LesseeOperatingSubleaseSubleasedArea"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_79e0a9ac-acf9-4db4-9911-827d0307229a" xlink:to="loc_yten_LesseeOperatingSubleaseSubleasedArea_b75cf73f-12b3-4179-86e8-2672832951e6" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_edb0b062-87a9-43ff-9bbb-156bc4d538c9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_79e0a9ac-acf9-4db4-9911-827d0307229a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_edb0b062-87a9-43ff-9bbb-156bc4d538c9" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_d712b312-b372-4b22-be6b-5becf44f7e80" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_79e0a9ac-acf9-4db4-9911-827d0307229a" xlink:to="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_d712b312-b372-4b22-be6b-5becf44f7e80" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_6e3be31b-67e7-486a-85d4-f36aebfe8ba7" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_LegalEntityAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_d712b312-b372-4b22-be6b-5becf44f7e80" xlink:to="loc_dei_LegalEntityAxis_6e3be31b-67e7-486a-85d4-f36aebfe8ba7" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_6e3be31b-67e7-486a-85d4-f36aebfe8ba7_default" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_6e3be31b-67e7-486a-85d4-f36aebfe8ba7" xlink:to="loc_dei_EntityDomain_6e3be31b-67e7-486a-85d4-f36aebfe8ba7_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_19eaa8b1-2412-4d83-aae0-1a25793bde2d" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_6e3be31b-67e7-486a-85d4-f36aebfe8ba7" xlink:to="loc_dei_EntityDomain_19eaa8b1-2412-4d83-aae0-1a25793bde2d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_CJCheilJedangCorporationMember_058db621-660f-4c56-ba72-1fbe9a2ed503" xlink:href="yten-20220930.xsd#yten_CJCheilJedangCorporationMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_19eaa8b1-2412-4d83-aae0-1a25793bde2d" xlink:to="loc_yten_CJCheilJedangCorporationMember_058db621-660f-4c56-ba72-1fbe9a2ed503" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis_4f76b4f8-4d6c-4c25-bbdc-1be5c6cc41c2" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_d712b312-b372-4b22-be6b-5becf44f7e80" xlink:to="loc_srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis_4f76b4f8-4d6c-4c25-bbdc-1be5c6cc41c2" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_4f76b4f8-4d6c-4c25-bbdc-1be5c6cc41c2_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis_4f76b4f8-4d6c-4c25-bbdc-1be5c6cc41c2" xlink:to="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_4f76b4f8-4d6c-4c25-bbdc-1be5c6cc41c2_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_11d36ab5-c18d-409b-bb7c-8b7e3b305083" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationDescriptionOfPropertyAxis_4f76b4f8-4d6c-4c25-bbdc-1be5c6cc41c2" xlink:to="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_11d36ab5-c18d-409b-bb7c-8b7e3b305083" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_PresidentialWayWoburnMassachusettsMember_1ddd0757-db14-422c-83fb-3e18301c3dd0" xlink:href="yten-20220930.xsd#yten_PresidentialWayWoburnMassachusettsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_11d36ab5-c18d-409b-bb7c-8b7e3b305083" xlink:to="loc_yten_PresidentialWayWoburnMassachusettsMember_1ddd0757-db14-422c-83fb-3e18301c3dd0" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_A410DowneyRoadAnd110GymnasiumPlaceMember_09172a8e-e1ff-453c-9ac2-b3bac1ca6d80" xlink:href="yten-20220930.xsd#yten_A410DowneyRoadAnd110GymnasiumPlaceMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RealEstateAndAccumulatedDepreciationNameOfPropertyDomain_11d36ab5-c18d-409b-bb7c-8b7e3b305083" xlink:to="loc_yten_A410DowneyRoadAnd110GymnasiumPlaceMember_09172a8e-e1ff-453c-9ac2-b3bac1ca6d80" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/COMMITMENTSANDCONTINGENCIESDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#COMMITMENTSANDCONTINGENCIESDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/COMMITMENTSANDCONTINGENCIESDetails" xlink:type="extended" id="ic9cf813cbde64622a08a19c3f852225f_COMMITMENTSANDCONTINGENCIESDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_57b8fd9c-8695-4854-91cb-aa60aa06a145" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_CollaborativeArrangementQuarterlyResearchFundingAndOptionFeesPayable_4153e9ea-c452-4e4d-84a9-5880aee3f3aa" xlink:href="yten-20220930.xsd#yten_CollaborativeArrangementQuarterlyResearchFundingAndOptionFeesPayable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_57b8fd9c-8695-4854-91cb-aa60aa06a145" xlink:to="loc_yten_CollaborativeArrangementQuarterlyResearchFundingAndOptionFeesPayable_4153e9ea-c452-4e4d-84a9-5880aee3f3aa" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_CollaborativeArrangementTerm_969ba0b9-363d-49ec-9571-58bac897fe3b" xlink:href="yten-20220930.xsd#yten_CollaborativeArrangementTerm"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_57b8fd9c-8695-4854-91cb-aa60aa06a145" xlink:to="loc_yten_CollaborativeArrangementTerm_969ba0b9-363d-49ec-9571-58bac897fe3b" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_CollaborativeArrangementResearchFundingAndOptionFeesPayable_5d1289f0-29ae-42fa-ba1b-330cc78cfae1" xlink:href="yten-20220930.xsd#yten_CollaborativeArrangementResearchFundingAndOptionFeesPayable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_57b8fd9c-8695-4854-91cb-aa60aa06a145" xlink:to="loc_yten_CollaborativeArrangementResearchFundingAndOptionFeesPayable_5d1289f0-29ae-42fa-ba1b-330cc78cfae1" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_CollaborativeArrangementTermOfOptionForLicenseAgreement_3193350b-2ac8-44fc-8ac0-f8a4cd0f7d86" xlink:href="yten-20220930.xsd#yten_CollaborativeArrangementTermOfOptionForLicenseAgreement"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_57b8fd9c-8695-4854-91cb-aa60aa06a145" xlink:to="loc_yten_CollaborativeArrangementTermOfOptionForLicenseAgreement_3193350b-2ac8-44fc-8ac0-f8a4cd0f7d86" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_dce3edae-98d5-4870-a682-4199a0f9ba7e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CollaborativeArrangementsAndNoncollaborativeArrangementTransactionsLineItems_57b8fd9c-8695-4854-91cb-aa60aa06a145" xlink:to="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_dce3edae-98d5-4870-a682-4199a0f9ba7e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_e2a1e463-b0cd-4cfc-ad85-b5fecd0d3652" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TypeOfArrangementAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCollaborativeArrangementsAndNoncollaborativeArrangementTransactionsTable_dce3edae-98d5-4870-a682-4199a0f9ba7e" xlink:to="loc_us-gaap_TypeOfArrangementAxis_e2a1e463-b0cd-4cfc-ad85-b5fecd0d3652" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_e2a1e463-b0cd-4cfc-ad85-b5fecd0d3652_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_e2a1e463-b0cd-4cfc-ad85-b5fecd0d3652" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_e2a1e463-b0cd-4cfc-ad85-b5fecd0d3652_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_8db20879-1b1e-4b84-b10d-8c2c8b88697c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_e2a1e463-b0cd-4cfc-ad85-b5fecd0d3652" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_8db20879-1b1e-4b84-b10d-8c2c8b88697c" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollaborativeArrangementMember_a83cfbd7-c52e-46a5-ac39-ce8dac9552b0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CollaborativeArrangementMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_8db20879-1b1e-4b84-b10d-8c2c8b88697c" xlink:to="loc_us-gaap_CollaborativeArrangementMember_a83cfbd7-c52e-46a5-ac39-ce8dac9552b0" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCommitmentsLineItems_b940c22d-9416-4d83-8178-fd0150b823ea" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherCommitmentsLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCommitment_54a6445a-514c-4a51-a4c7-8237f191e9da" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherCommitment"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_b940c22d-9416-4d83-8178-fd0150b823ea" xlink:to="loc_us-gaap_OtherCommitment_54a6445a-514c-4a51-a4c7-8237f191e9da" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCommitmentsTable_fe9d7844-874b-4e5a-8d5b-89d0cfd9c113" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherCommitmentsTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_b940c22d-9416-4d83-8178-fd0150b823ea" xlink:to="loc_us-gaap_OtherCommitmentsTable_fe9d7844-874b-4e5a-8d5b-89d0cfd9c113" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCommitmentsAxis_3fadc1fb-183d-41fb-8a63-fb1fc42207fd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherCommitmentsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OtherCommitmentsTable_fe9d7844-874b-4e5a-8d5b-89d0cfd9c113" xlink:to="loc_us-gaap_OtherCommitmentsAxis_3fadc1fb-183d-41fb-8a63-fb1fc42207fd" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCommitmentsDomain_3fadc1fb-183d-41fb-8a63-fb1fc42207fd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherCommitmentsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_OtherCommitmentsAxis_3fadc1fb-183d-41fb-8a63-fb1fc42207fd" xlink:to="loc_us-gaap_OtherCommitmentsDomain_3fadc1fb-183d-41fb-8a63-fb1fc42207fd_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCommitmentsDomain_b8333a89-9965-469a-8f19-5fae239c12ef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherCommitmentsDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_OtherCommitmentsAxis_3fadc1fb-183d-41fb-8a63-fb1fc42207fd" xlink:to="loc_us-gaap_OtherCommitmentsDomain_b8333a89-9965-469a-8f19-5fae239c12ef" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_GrainProductionGuaranteedPaymentsMember_02112139-47ce-4ceb-82d1-81fa70d4fbab" xlink:href="yten-20220930.xsd#yten_GrainProductionGuaranteedPaymentsMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsDomain_b8333a89-9965-469a-8f19-5fae239c12ef" xlink:to="loc_yten_GrainProductionGuaranteedPaymentsMember_02112139-47ce-4ceb-82d1-81fa70d4fbab" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/GEOGRAPHICINFORMATIONDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#GEOGRAPHICINFORMATIONDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/GEOGRAPHICINFORMATIONDetails" xlink:type="extended" id="i1d3cdfed34214f2388ae6efeb839e9cf_GEOGRAPHICINFORMATIONDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_e696dddb-bb6e-426f-8867-067bf84d468a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_94784732-b558-49a3-bbd2-9718891b6108" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_e696dddb-bb6e-426f-8867-067bf84d468a" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_94784732-b558-49a3-bbd2-9718891b6108" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_146a1959-2423-440f-97a7-c7fc96dbc1ab" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_e696dddb-bb6e-426f-8867-067bf84d468a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_146a1959-2423-440f-97a7-c7fc96dbc1ab" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_4f31b3fa-b296-4017-9636-663348dffc8c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_e696dddb-bb6e-426f-8867-067bf84d468a" xlink:to="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_4f31b3fa-b296-4017-9636-663348dffc8c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_d0e49601-68fb-4889-94fc-4ea973939239" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_StatementGeographicalAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_4f31b3fa-b296-4017-9636-663348dffc8c" xlink:to="loc_srt_StatementGeographicalAxis_d0e49601-68fb-4889-94fc-4ea973939239" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_d0e49601-68fb-4889-94fc-4ea973939239_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_SegmentGeographicalDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_d0e49601-68fb-4889-94fc-4ea973939239" xlink:to="loc_srt_SegmentGeographicalDomain_d0e49601-68fb-4889-94fc-4ea973939239_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_1040cd61-c190-482b-b83a-36228ca69199" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_SegmentGeographicalDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_d0e49601-68fb-4889-94fc-4ea973939239" xlink:to="loc_srt_SegmentGeographicalDomain_1040cd61-c190-482b-b83a-36228ca69199" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_country_US_9bc1db85-667d-44a8-8c01-54901b657950" xlink:href="https://xbrl.sec.gov/country/2022/country-2022.xsd#country_US"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_1040cd61-c190-482b-b83a-36228ca69199" xlink:to="loc_country_US_9bc1db85-667d-44a8-8c01-54901b657950" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_country_CA_f3742921-ca0b-4f2e-923e-e79772070548" xlink:href="https://xbrl.sec.gov/country/2022/country-2022.xsd#country_CA"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_1040cd61-c190-482b-b83a-36228ca69199" xlink:to="loc_country_CA_f3742921-ca0b-4f2e-923e-e79772070548" xlink:type="arc" order="1"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/CAPITALSTOCKANDWARRANTSNarrativeDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#CAPITALSTOCKANDWARRANTSNarrativeDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/CAPITALSTOCKANDWARRANTSNarrativeDetails" xlink:type="extended" id="i926be82c675d46edbc197e5141f991ff_CAPITALSTOCKANDWARRANTSNarrativeDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfStockLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_8058cedb-5427-468a-99a8-32f83e7f3c0f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_8058cedb-5427-468a-99a8-32f83e7f3c0f" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockPricePerShare_7629ac21-c13c-4896-b01e-8a52b7379983" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockPricePerShare"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_us-gaap_SaleOfStockPricePerShare_7629ac21-c13c-4896-b01e-8a52b7379983" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_7e4a69d0-b6a5-493c-b7fd-e080ea9753b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockConsiderationReceivedOnTransaction"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_7e4a69d0-b6a5-493c-b7fd-e080ea9753b3" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfStockIssuanceCosts_c560c071-35a7-4ee7-ba52-784344ac123c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsOfStockIssuanceCosts"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_us-gaap_PaymentsOfStockIssuanceCosts_c560c071-35a7-4ee7-ba52-784344ac123c" xlink:type="arc" order="3"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_c3958c51-27d2-4f91-b52b-7ee761d49303" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PreferredStockSharesAuthorized"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_c3958c51-27d2-4f91-b52b-7ee761d49303" xlink:type="arc" order="4"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_0aa04631-7f3a-43a6-906e-55c74ad4bbb9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_0aa04631-7f3a-43a6-906e-55c74ad4bbb9" xlink:type="arc" order="5"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_ClassOfWarrantOrRightExpired_30ce5bd9-f722-4940-87fa-0e378e54d523" xlink:href="yten-20220930.xsd#yten_ClassOfWarrantOrRightExpired"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_yten_ClassOfWarrantOrRightExpired_30ce5bd9-f722-4940-87fa-0e378e54d523" xlink:type="arc" order="6"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_ClassOfWarrantOrRightExercised_8166f3f7-0f0b-40d4-99d5-8efdefd91003" xlink:href="yten-20220930.xsd#yten_ClassOfWarrantOrRightExercised"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_yten_ClassOfWarrantOrRightExercised_8166f3f7-0f0b-40d4-99d5-8efdefd91003" xlink:type="arc" order="7"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromWarrantExercises_9dc0855d-d390-49ae-8db6-c1f9f08d2b0b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromWarrantExercises"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_us-gaap_ProceedsFromWarrantExercises_9dc0855d-d390-49ae-8db6-c1f9f08d2b0b" xlink:type="arc" order="8"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_c1120ad6-006a-4f64-96bd-15a1f2cf137d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfStockByClassTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_9c5ddaf7-3351-4d24-9613-7396dc7b7152" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_c1120ad6-006a-4f64-96bd-15a1f2cf137d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_e9647ac4-f532-43dd-9f4e-506be42423cb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_c1120ad6-006a-4f64-96bd-15a1f2cf137d" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_e9647ac4-f532-43dd-9f4e-506be42423cb" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_e9647ac4-f532-43dd-9f4e-506be42423cb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_e9647ac4-f532-43dd-9f4e-506be42423cb" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_e9647ac4-f532-43dd-9f4e-506be42423cb_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_624e139a-2a23-4839-83fd-14757d51089e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_e9647ac4-f532-43dd-9f4e-506be42423cb" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_624e139a-2a23-4839-83fd-14757d51089e" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_PublicOfferingMember_52060de2-a6dc-4e43-98d5-b0fedcf7e223" xlink:href="yten-20220930.xsd#yten_PublicOfferingMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_624e139a-2a23-4839-83fd-14757d51089e" xlink:to="loc_yten_PublicOfferingMember_52060de2-a6dc-4e43-98d5-b0fedcf7e223" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_3600c379-73dc-40d4-808c-aa6d4e9a4626" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_c1120ad6-006a-4f64-96bd-15a1f2cf137d" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_3600c379-73dc-40d4-808c-aa6d4e9a4626" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_3600c379-73dc-40d4-808c-aa6d4e9a4626_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_3600c379-73dc-40d4-808c-aa6d4e9a4626" xlink:to="loc_us-gaap_EquityComponentDomain_3600c379-73dc-40d4-808c-aa6d4e9a4626_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_5d7225c3-63c2-407d-9df9-8b9dae7c52bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_3600c379-73dc-40d4-808c-aa6d4e9a4626" xlink:to="loc_us-gaap_EquityComponentDomain_5d7225c3-63c2-407d-9df9-8b9dae7c52bb" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_54f9ac13-9b30-48d4-b4aa-13f002b23a98" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_5d7225c3-63c2-407d-9df9-8b9dae7c52bb" xlink:to="loc_us-gaap_CommonStockMember_54f9ac13-9b30-48d4-b4aa-13f002b23a98" xlink:type="arc" order="0"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/CAPITALSTOCKANDWARRANTSScheduleOfWarrantsIssuanceDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#CAPITALSTOCKANDWARRANTSScheduleOfWarrantsIssuanceDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/CAPITALSTOCKANDWARRANTSScheduleOfWarrantsIssuanceDetails" xlink:type="extended" id="i7bb669f52b624cb29fb60d11c7f78124_CAPITALSTOCKANDWARRANTSScheduleOfWarrantsIssuanceDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_14bee099-8638-458f-aca5-ed2995ffe803" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfStockLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_e6bcb137-5619-4587-b884-8cc5ad9b8354" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_14bee099-8638-458f-aca5-ed2995ffe803" xlink:to="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_e6bcb137-5619-4587-b884-8cc5ad9b8354" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_4cf65c1f-a1ca-41b6-a349-56163106a74d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_14bee099-8638-458f-aca5-ed2995ffe803" xlink:to="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_4cf65c1f-a1ca-41b6-a349-56163106a74d" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_f7777827-c0e6-4d88-8636-9d97764db2e1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfStockByClassTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_14bee099-8638-458f-aca5-ed2995ffe803" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_f7777827-c0e6-4d88-8636-9d97764db2e1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_7f07d491-07e4-42a0-827c-0d7ff21bb65a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_f7777827-c0e6-4d88-8636-9d97764db2e1" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_7f07d491-07e4-42a0-827c-0d7ff21bb65a" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_7f07d491-07e4-42a0-827c-0d7ff21bb65a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_7f07d491-07e4-42a0-827c-0d7ff21bb65a" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_7f07d491-07e4-42a0-827c-0d7ff21bb65a_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_7132f1cb-8b06-4641-b8cc-37fca27628b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_7f07d491-07e4-42a0-827c-0d7ff21bb65a" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_7132f1cb-8b06-4641-b8cc-37fca27628b3" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_PublicOfferingMember_7132f2e5-f0d5-4777-8344-f9b3efcac06a" xlink:href="yten-20220930.xsd#yten_PublicOfferingMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_7132f1cb-8b06-4641-b8cc-37fca27628b3" xlink:to="loc_yten_PublicOfferingMember_7132f2e5-f0d5-4777-8344-f9b3efcac06a" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrivatePlacementMember_cdfb1c29-85c9-47d5-97a2-8f10ec630005" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PrivatePlacementMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_7132f1cb-8b06-4641-b8cc-37fca27628b3" xlink:to="loc_us-gaap_PrivatePlacementMember_cdfb1c29-85c9-47d5-97a2-8f10ec630005" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightAxis_5b80a28a-f0c9-4bf8-bc3f-d31e54ac2abd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfWarrantOrRightAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_f7777827-c0e6-4d88-8636-9d97764db2e1" xlink:to="loc_us-gaap_ClassOfWarrantOrRightAxis_5b80a28a-f0c9-4bf8-bc3f-d31e54ac2abd" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightDomain_5b80a28a-f0c9-4bf8-bc3f-d31e54ac2abd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfWarrantOrRightDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ClassOfWarrantOrRightAxis_5b80a28a-f0c9-4bf8-bc3f-d31e54ac2abd" xlink:to="loc_us-gaap_ClassOfWarrantOrRightDomain_5b80a28a-f0c9-4bf8-bc3f-d31e54ac2abd_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightDomain_484b70ab-4346-4c71-b640-19ce2a83474b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfWarrantOrRightDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ClassOfWarrantOrRightAxis_5b80a28a-f0c9-4bf8-bc3f-d31e54ac2abd" xlink:to="loc_us-gaap_ClassOfWarrantOrRightDomain_484b70ab-4346-4c71-b640-19ce2a83474b" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_SeriesBWarrantExpiringMay2027Member_f0c6cc8b-58c8-48c1-ac15-906ee40e8ab9" xlink:href="yten-20220930.xsd#yten_SeriesBWarrantExpiringMay2027Member"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfWarrantOrRightDomain_484b70ab-4346-4c71-b640-19ce2a83474b" xlink:to="loc_yten_SeriesBWarrantExpiringMay2027Member_f0c6cc8b-58c8-48c1-ac15-906ee40e8ab9" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_SeriesAWarrantExpiringDecember2022Member_70dde283-ff59-4e0a-ab25-22b58c96b163" xlink:href="yten-20220930.xsd#yten_SeriesAWarrantExpiringDecember2022Member"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfWarrantOrRightDomain_484b70ab-4346-4c71-b640-19ce2a83474b" xlink:to="loc_yten_SeriesAWarrantExpiringDecember2022Member_70dde283-ff59-4e0a-ab25-22b58c96b163" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_WarrantExpiringJanuary2024Member_66a71414-1188-4342-9ea4-889f6392cbc2" xlink:href="yten-20220930.xsd#yten_WarrantExpiringJanuary2024Member"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfWarrantOrRightDomain_484b70ab-4346-4c71-b640-19ce2a83474b" xlink:to="loc_yten_WarrantExpiringJanuary2024Member_66a71414-1188-4342-9ea4-889f6392cbc2" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_yten_WarrantExpiringSeptember2024Member_82caf600-d6f0-42ab-bb37-6682adf0d65f" xlink:href="yten-20220930.xsd#yten_WarrantExpiringSeptember2024Member"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfWarrantOrRightDomain_484b70ab-4346-4c71-b640-19ce2a83474b" xlink:to="loc_yten_WarrantExpiringSeptember2024Member_82caf600-d6f0-42ab-bb37-6682adf0d65f" xlink:type="arc" order="3"/>
</link:definitionLink>
<link:roleRef roleURI="http://www.yield10bio.com/role/CAPITALSTOCKANDWARRANTSScheduleofCommonStockReservedForFutureIssuanceDetails" xlink:type="simple" xlink:href="yten-20220930.xsd#CAPITALSTOCKANDWARRANTSScheduleofCommonStockReservedForFutureIssuanceDetails"/>
<link:definitionLink xlink:role="http://www.yield10bio.com/role/CAPITALSTOCKANDWARRANTSScheduleofCommonStockReservedForFutureIssuanceDetails" xlink:type="extended" id="iaba0f54ab5bd4ab5802e7a716d4ff149_CAPITALSTOCKANDWARRANTSScheduleofCommonStockReservedForFutureIssuanceDetails">
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_bc48dd0e-1052-428e-821f-5d7c0772931f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfStockLineItems"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_e8571d44-6f24-4e84-9941-ba2687a4b7b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_bc48dd0e-1052-428e-821f-5d7c0772931f" xlink:to="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_e8571d44-6f24-4e84-9941-ba2687a4b7b3" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_029caedc-f91c-46c7-9284-d9b13d0f72a3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfStockByClassTable"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_bc48dd0e-1052-428e-821f-5d7c0772931f" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_029caedc-f91c-46c7-9284-d9b13d0f72a3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightAxis_7276a4e8-2a8f-4407-b78d-93836016aa80" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfWarrantOrRightAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_029caedc-f91c-46c7-9284-d9b13d0f72a3" xlink:to="loc_us-gaap_ClassOfWarrantOrRightAxis_7276a4e8-2a8f-4407-b78d-93836016aa80" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightDomain_7276a4e8-2a8f-4407-b78d-93836016aa80_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfWarrantOrRightDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ClassOfWarrantOrRightAxis_7276a4e8-2a8f-4407-b78d-93836016aa80" xlink:to="loc_us-gaap_ClassOfWarrantOrRightDomain_7276a4e8-2a8f-4407-b78d-93836016aa80_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightDomain_ad6341ed-252c-4d32-aeb6-867519e7f479" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfWarrantOrRightDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ClassOfWarrantOrRightAxis_7276a4e8-2a8f-4407-b78d-93836016aa80" xlink:to="loc_us-gaap_ClassOfWarrantOrRightDomain_ad6341ed-252c-4d32-aeb6-867519e7f479" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_WarrantMember_96565c19-736e-4025-9eed-1b42dc746769" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WarrantMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfWarrantOrRightDomain_ad6341ed-252c-4d32-aeb6-867519e7f479" xlink:to="loc_us-gaap_WarrantMember_96565c19-736e-4025-9eed-1b42dc746769" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_8c743387-3414-49f9-9f72-71883d4a4c87" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AwardTypeAxis"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_029caedc-f91c-46c7-9284-d9b13d0f72a3" xlink:to="loc_us-gaap_AwardTypeAxis_8c743387-3414-49f9-9f72-71883d4a4c87" xlink:type="arc" order="2"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8c743387-3414-49f9-9f72-71883d4a4c87_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_8c743387-3414-49f9-9f72-71883d4a4c87" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8c743387-3414-49f9-9f72-71883d4a4c87_default" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_38615048-78c6-4bfe-a46d-db81cd3dacb2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_8c743387-3414-49f9-9f72-71883d4a4c87" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_38615048-78c6-4bfe-a46d-db81cd3dacb2" xlink:type="arc" order="1"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_f22a1f39-057b-428a-b849-00ce1311e7e5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeStockOptionMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_38615048-78c6-4bfe-a46d-db81cd3dacb2" xlink:to="loc_us-gaap_EmployeeStockOptionMember_f22a1f39-057b-428a-b849-00ce1311e7e5" xlink:type="arc" order="0"/>
<link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_a11fa76e-0804-474d-a896-703725f53af6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
<link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_38615048-78c6-4bfe-a46d-db81cd3dacb2" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_a11fa76e-0804-474d-a896-703725f53af6" xlink:type="arc" order="1"/>
</link:definitionLink>
</link:linkbase>

1 Subsequent Filing that References this Filing

  As Of               Filer                 Filing    For·On·As Docs:Size             Issuer                      Filing Agent

 1/24/23  YIELD10 Bioscience, Inc.          424B5                  1:587K

2 Previous Filings that this Filing References

  As Of               Filer                 Filing    For·On·As Docs:Size             Issuer                      Filing Agent

 5/11/22  YIELD10 Bioscience, Inc.          10-Q        3/31/22   59:4.5M
 3/25/22  YIELD10 Bioscience, Inc.          10-K       12/31/21   83:9.4M
↑Top
Filing Submission 0001121702-22-000049   –   Alternative Formats (Word / Rich Text, HTML, Plain Text, et al.)
Copyright © 2024 Fran Finnegan & Company LLC – All Rights Reserved.
About — Privacy — Redactions — Help — Tue., Apr. 30, 6:09:33.1am ET